Add time:09/28/2019 Source:sciencedirect.com
2-Chloropurine (cas 1681-15-8) is attacked by mammalian xanthine oxidase simultaneously at C-6 and C-8. The ratio of the two rates (=1) is insensitive to variations of pH or temperature. Experiments with 2-chloropurine as inhibitor indicate instantaneous association of this purine with the active center of the enzyme. It is concluded that the rate-determining step, viz., conversion of ES → (ES)∗, is common to both pathways and that only in the subsequent interaction with water does the route fork.
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