Add time:09/25/2019         Source:infona.pl

The effects of vitamin K (VK) derivatives VK 3 and VK 5 on human immune cells have not been extensively investigated. We examined the effects of VK 3 and VK 5 on proliferation, apoptosis, cytokine production, and CD4+CD25+Foxp3+ regulatory T (Treg) cell-frequency in human peripheral blood mononuclear cells (PBMCs) activated by T cell mitogen in vitro.Anti-proliferative effects of VK 3 and VK 5 on T-cell mitogen activated PBMCs were assessed by WST assay procedures. Apoptotic cells were determined as Annexin V positive/propidium iodide (PI) negative cells. Cytokine concentrations in the supernatant of the culture medium were measured with bead-array procedures followed by analysis with flow cytometry. The CD4+CD25+Foxp3+Treg cells in mitogen-activated PBMCs were stained with fluorescence-labeled specific antibodies followed by flow cytometry.VK 3 and VK 5 suppressed the mitogen-activated proliferation of PBMCs significantly at 10–100μM (p<0.05). The data also suggest that VK 3 and VK 5 promote apoptosis in the mitogen-activated T cells. VK 3 and VK 5 significantly inhibited the production of tumor necrosis factor (TNF) α, interleukin (IL)-4, -6, and -10 from the activated PBMCs at 10–100μM (p<0.05). In contrast, VK 3 and VK 5 significantly increased Treg cell-frequency in the activated PBMCs at concentrations more than 10μM (p<0.001).Our data suggest that VK 3 and VK 5 attenuate T cell mediated immunity by inhibiting the proliferative response and inducing apoptosis in activated T cells.

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