Add time:09/29/2019         Source:infona.pl

. A resonance light scattering technique has been developed in order to determine potassium ion in serum. Potassium ion was found to bind the tetraphenylboronate anion [(C6H5)4B]− in acetate buffer (pH 8.0) in the presence of sodium dodecyl benzene sulfonate as a stabilizer, forming the B(C6H5)4-K aggregate which produces intense resonance scattering light. Effects of factors such as acidity, ionic strength and interferents on the RLS of B(C6H5)4-K were investigated. The solution pH close to neutral facilitates the production of RLS, and few biologically relevant species interfere in the determination of potassium ion. The resonance scattering light intensity at the maximum peak of 567 nm was linear to the concentration of potassium ion in the range of 0.2–2.0 µg mL−1 with a detection limit of 20.0 ng mL−1. The method was applied to determine trace amounts of potassium ion in serum and showed high sensitivity and accuracy compared with the clinically used ion-selective electrode method.

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