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We used a battery of different methods to study the association in aqueous Sodium dehydrocholate (cas 145-41-5) (NaDHC) solutions. This salt associates by a stepwise mechanism. Below (9.64.2)104 moldm3 there is a molecular solution with some strongly insoluble dehydrocholic acid produced by hydrolysis. Between (9.64.2)104 and (5.22.2)103moldm3, an aggregate similar to acid soap (NaDHC.HDHC) appears and its amount and the aggregates size increase with concentration. At =(2.200.85)102moldm3 the aggregates formed have properties usually associated with true micelles, such as solubilisation of water-insoluble dyes. These aggregates increase in size with concentration and change their shape at 8102moldm3, giving nonsymmetrical aggregates. The changes in the solution physicochemical properties at these concentrations may be misinterpreted and this explains the different values of the critical micelle concentration reported in the literature for substances with similar structure, such as bile salts.
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