Add time:09/28/2019         Source:infona.pl

A cyclodextrin-modified micellar electrokinetic chromatographic method was established to determine five anthraquinone derivatives and a distyrene derivative in Rhubarb. The six components were successfully separated by using the mixed micellar system consisting of 20mmol/l SC and 20mmol/l STC with 15mmol/l β-CD in the BGE of 20mmol/l borax buffer (pH 11) in less than 20min. Effect of SC and STC concentrations, pH of BGE and concentration of β-CD on the separation was studied. The analytical performance of the method was discussed in terms of linearity response, precision, detection limits, quantitation limits and recoveries. The method developed was applied to the determination of three commercial Rhubarb samples. The results obtained agreed with those of the reported documents.

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