Add time:09/28/2019         Source:infona.pl

A validated, quantitative LC–APCI-MS/MS method for methadone, EDDP and EMDP in 200-μL plasma is presented. Specimen preparation was limited to protein precipitation and centrifugation. Chromatographic separation was achieved on a Synergi Hydro-RP 80A (50mm×2.0mm, 4μm) column with gradient elution. The assay was linear from 1 to 500ng/mL, with intra- and inter-assay accuracy ≥87.5% and intra- and inter-assay precision <13.4% R.S.D. and recovery ≥87.5% for all analytes at 40ng/mL. This analytical method is suitable for the accurate and precise determination of methadone and metabolites in human plasma specimens.

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