Terbium oxide doped molybdenum oxide (MoO3:Tb) nanostructures have successfully been grown by radio frequency (RF) magnetron sputtering. MoO3 nanostructures containing 1, 3, 5 and 7 wt% of terbium oxide are grown on glass substrates. X-ray diffraction (XRD) analysis shows that the MoO3:Tb nanost...

Quantitative and qualitative determination of sucrose from complex tastant mixtures using Cu foam electrode was investigated in this study. Cu foam was prepared and its three-dimensional (3-D) mesh structure was characterized by scanning electron microscopy (SEM). Cu foam was utilized as working...

The reversed-phase liquid chromatography retention of a series of benzoate ester molecules was investigated over a wide range of sucrose concentrations (c) 0.01–0.8 M using a C18 as the stationary phase and a methanol–water mixture with high organic fraction (60/40, v/v) as the mobile phase. A...

Intergeneric protoplast fusion was performed between Pseudomonas putida, a strain with moderate benzoate tolerance and degradation ability (

(±)-Sodium5-bromo-2-(α-hydroxypentyl) benzoate (brand name: brozopine, BZP, 1a), derived from 1 to 3-n-Butylphthalide (NBP), has been reported to protect the brain from stoke and has been approved by CFDA in Phase I-II clinical trials. However, it remains to be investigated whether 1a may exhi...

Lepidopteran insect pest management has relied heavily on synthetic chemical pesticides, but their efficiency is declining as a result of emerging insecticide resistance. Recently biopesticides have become the most promising products employed in pest management strategies. We investigated the su...

The feasibility of producing proliposomes containing curcumin, as well as liposome dispersions, using different mixtures of purified and nonpurified soybean phospholipids was studied. Proliposomes were produced through coating of micronized sucrose and physicochemically characterized over 30 day...

This work studied the ultrasound technology as an alternative to enhance the reaction of sucrose inversion. To achieve it, the effect of ultrasound processing was evaluated in three situations: the reaction (invertase acting on substrate under ultrasound processing), the isolated substrate (sucr...

The assembly pattern of acetate units and methyl groups in the biosynthesis of a polyketide, pyrenulic acid C (1), in cultured mycobionts of a Vietnamese Pyrenula sp. was verified by administration of sodium [1-13C]-acetate, sodium [1,2-13C2]-acetate, and L-[methyl-13C]-methionine. The cultured ...

SummaryThe complete amino acid sequence of stellacyanin, a blue copper-containing glycoprotein, is presented. The protein has been enzymatically cleaved by trypsin, chymotrypsin, and by the protease from the Staphylococcus aureus strain U8. Stellacyanin consists of a single polypeptide chain of ...

The loop of chelating residues places constraints on the metal ion environment in proteins. The high reduction potentials of the class of blue copper proteins that make possible their electron transfer functon, as well as the variations in the reduction potentials of the different blue copper pr...

We have measured the optical absorption spectra of azurin and stellacyanin in the wavelength range 1100-350 nm and in the temperature interval 290–20 K. Samples are protein aqueous solutions containing 65% (v/v) glycerol or ethylene glycol as cryoprotectants and remain homogeneous and transpare...

The complete amino-acid sequence of mavicyanin, a small blue copper-containing glycoprotein isolated from zucchini peelings, is presented. The sequence of this cupredoxin was deduced from analysis of peptides obtained after cleavage of the protein with trypsin or Asp-N endoproteinase. Mavicyanin...

Stellacyanin, the single blue copper protein from Rhusvernicifera, is reduced stoichiometrically by Cr(II)aq ions yielding a 1:1 adduct between the Cr(III) produced and the reduced protein. This Cr(III)-labeled stellacyanin is substitution inert and no significant loss of the label occurs during...

The 1H NMR spectrum of Co(II) stellacyanin is reported, in which four signals not previously observed have been detected. NOE experiments were performed to assign the hyperfine shifted signals corresponding to a Cys and two His residues. Both His residues are solvent-accessible and are shown to ...

Extended X-ray absorption fine structure (EXAFS) studies of Cu(II) (oxidized), Cu(I) (reduced), Ni(II) and Co(II) stellacyanin from Rhus vernicifera are reported. For Cu(II) stellacyanin, the coordination by three close ligands, viz. 2 N and 1 S, with the presence of smaller shells pointing to i...

Reaction of Cu(II)-stellacyanin with excess (NH3)5Ru(H2O)2+ yields a protein to which (NH3)5Ru(III) is coordinated. The binding stoichiometry of Ru:Cu is (2.1 ± 0.1):1 as shown by ICP atomic emission measurements. In the 1H-NMR spectrum of the Ru(III)-labeled protein, the signals from the C-2H ...

Stellacyanin from Rhus vernificera is a blue copper protein in which the metal is coordinated to a Cys, two His and a Gln residue. We have studied the Ni(II) derivative of stellacyanin by paramagnetic 1H NMR spectroscopy. The NMR spectrum resembles that of Ni(II) Met121Gln azurin, indicating a s...

A three-dimensional model of the “blue” copper-glycoprotein stellacyanin from Rhus vernicifera has been derived by computer graphics, energy minimization and molecular dynamics techniques. The initial atomic co-ordinates were obtained by making substitutions and insertions in the known structu...

The efficiency of silicon removal technology by acid leaching and flocculation during zirconium oxychloride octahydrate (ZrOCl2⋅8 H2O) production has been improved. The optimum conditions of acid leaching were found to be as follows: transiton material, 300 g; HCl (6 mol/L), 600 mL; leaching tim...