Hepatocellular carcinoma (HCC) is one of the most deadly cancers worldwide. We performed a proteomic study to understand the molecular mechanisms underlying metastasis in HCC. Among the 3491 protein spots observed by two-dimensional difference gel electrophoresis (2D-DIGE), we found that 197 and...

The metabolism of [14C]7-methylbenz[c]acridine, a weakly carcinogenic polycyclic aza aromatic hydrocarbon, was studied in rats in vivo, and in rat hepatocytes and hepatic microsomes. Evidence for oxidation of the methyl group and excretion of 7-hydroxymethylbenz[c]acridine and benz[c]acridine-7-...

The metabolism of the weak carcinogen 7-methylbenz[c]acridine (7MBAC) was examined in rat liver microsomes from 3-methylcholanthrene(MC)-induced animals by the use of mixed 14C- and 2H-labelled substrate. The three metabolites identified by spectroscopic and chromatographic examination were 7-OH...

The SP-isomer of 5′-OH-N4-benzoyl-2′-deoxycytidine-3′-O-(2-thio-4,4-pentamethylene-1,3,2-oxathiaphospholane) undergoes DBU-promoted intramolecular cyclization providing as a sole product SP-deoxycytidine cyclic 3′,5′-O,O-phosphorothioate. Unexpectedly, the RP-counterpart yields a mixture of...

It has been found that 2′deoxycytidine (dCyd) crystallizes in different forms, denoted dCyd A, dCyd B and dCyd C·H2O.In the crystal lattice of the newly discovered form dCydC·H2O there is a crystalline water molecule present. The monohydrate of dCydC, under suitable conditions (RT, over P2O5,...

This work has undertaken liquid chromatographic separation of nucleosides and deoxynucleosides. Two different columns with three mobile phases (A, deionized water; B, 50 mM phosphate buffer (pH 4.0); C, methanol) and slightly different gradient programs were used. The elution order was as follow...

Publisher SummaryThis chapter describes methods for preparation of S-adenosyl-methionine and corresponding derivatives. Like the mammalian liver, growing or metabolizing yeast synthesizes the corresponding S-adenosyl derivatives from methionine or ethionine in amounts ranging from 7 to 20 μmole...

The oxidation of methionine-CH3C14 and methionine-2-C14 to respiratory CO2 was greater in the female than in the male rat. The reverse was true for ethionine-C2H5-C14 and ethionine-2-C14. The presence of ethionine in the diet increased the oxidation of methionine to respiratory CO2, and the pr...

A method for the analysis of S-adenosyl-l-methionine (SAM) and S-adenosyl-l-ethionine (SAE) and their major metabolites by high-performance liquid chromatography is described. The procedure allows the simultaneous analysis of the natural polyamines, putrescine, spermidine, and spermine, and some...

A new complex N-(2-hydroxyethylamino)ethylsalicylaldiminato]-palladium(II) chloride [Pd(L)Cl] was synthesized by the reaction in ethanol of the Schiff base 2-(2-(2-hydroxyethylamino)ethylimino)methyl phenol (LH) and Li2PdCl4. The complex was characterized by 1H NMR, 13C NMR, FT-IR and UV–Vis sp...

We have developed a new class of PDE10A inhibitor, a pyrazolo[1,5-a]pyrimidine derivative MT-3014 (1). A previous compound introduced was deprioritized due to concerns for E/Z-isomerization and glutathione-adduct formation at the core stilbene structure. We discovered pyrazolo [1,5-a] pyrimidine...

Heterogeneous gold nanocluster catalysts immobilized by the method known as polymer incarceration were prepared. Polystyrene-derived polymers with epoxide and alcohol moieties, which could be cross-linked under heating conditions, were employed as supports for their preparation. Cationic gold sa...

A new preparation method for solid-supported crown ethers (CE) as lithium ion (Li+) adsorbents is presented. Hydroxy-dibenzo-14-crown-4 ether (HDB14C4) was immobilized on multi-walled carbon nanotubes (MWCNTs) through the following steps: (1) MWCNTs were oxidized to generate carboxyl groups (COO...

Densities and kinematic viscosities of polymer absorbents such as poly(propylene glycol) diglycidyl ether(PPDE) with molecular weights of 380 and 640, and poly(ethylene glycol) monooleate(PEGM) with molecular weight of 460 were respectively measured at atmosphere and temperatures ranging from 27...

Quaternary ammonium poly(arylene ether sulfone) copolymer random and multi-block ionomers were synthesized using multiple condensation reactions. Hydrophilic and hydrophobic block-length relationships were investigated with respect to directionally-dependent water swelling, hydroxyl conductivity...

A series of alkaline anion exchange membranes (AAEMs) are synthesized from poly (arylene ether ketone) -poly (vinyl-1-butylimidazolium) graft copolymers (PAEK-g-[PBVIm-OH]) with different [PBVIm-OH] cationic chain lengths. The chemical structure of the synthesized polymers is confirmed by 1H NMR...

A practical chromatography-free synthesis of a potent polo-like kinase-1 inhibitor possessing a unique 5,6-dihydroimidazolo[1,5-f]pteridine structure has been developed. We showed that key cyanoimidazole ring formation could be conducted at benign temperature and obtained a chiral 5,6-dihydroimi...

Enhancement of the piezoelectric response (d33) of aluminum nitride (AlN) was attempted by incorporating Mg and/or Ti into AlN. Addition of either Mg or Ti as a single dopant was unable to effectively increase the d33 of AlN. However, the d33 could be augmented when both Mg and Ti were codoped i...

In this paper, the hydrolysis behavior of AlN in the leaching process of secondary aluminum dross was studied through thermodynamic analysis, leaching tests, X-ray Fluorescence (XRF), X-ray diffraction (XRD) and Scanning Electron Microscope (SEM). The effects of reaction temperature and time on ...

Aluminum nitride (AlN) was synthesized by combustion of spherical Mg–Al alloy particles in air. Alloy powder was freely poured on a sample table, which formed a cone with a diameter of 1-2 cm. A commercial electro-thermal furnace was used to ignite the cone to start the synthesis. During the co...