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(S)-2-Benzyloxycarbonylamino-4-(4-nitro-phenylcarbamoyl)-butyric acid
L-glutamic acid 5-(4-nitroanilide)
Conditions | Yield |
---|---|
With hydrogen; palladium on activated charcoal In methanol |
(S)-4-[2-(4-Nitro-phenylcarbamoyl)-ethyl]-5-oxo-oxazolidine-3-carboxylic acid benzyl ester
L-glutamic acid 5-(4-nitroanilide)
Conditions | Yield |
---|---|
Multi-step reaction with 2 steps 1: lithium hydroxide / tetrahydrofuran; H2O / 0.5 h / 5 °C 2: hydrogen / Pd/C / methanol View Scheme |
Conditions | Yield |
---|---|
Multi-step reaction with 6 steps 1: 98 percent / triethylamine / methanol / 3 h 2: 90 percent / toluenesulfonic acid / toluene / 3 h / Heating 3: thionyl chloride / CH2Cl2 / 0.5 h / Heating 4: pyridine / CH2Cl2 / 0.25 h / 20 °C 5: lithium hydroxide / tetrahydrofuran; H2O / 0.5 h / 5 °C 6: hydrogen / Pd/C / methanol View Scheme |
Conditions | Yield |
---|---|
Multi-step reaction with 5 steps 1: 90 percent / toluenesulfonic acid / toluene / 3 h / Heating 2: thionyl chloride / CH2Cl2 / 0.5 h / Heating 3: pyridine / CH2Cl2 / 0.25 h / 20 °C 4: lithium hydroxide / tetrahydrofuran; H2O / 0.5 h / 5 °C 5: hydrogen / Pd/C / methanol View Scheme |
(S)-4-<2'-(Chloroformyl)ethyl>-5-oxooxazolidin-3-carbonsaeure-benzylester
L-glutamic acid 5-(4-nitroanilide)
Conditions | Yield |
---|---|
Multi-step reaction with 3 steps 1: pyridine / CH2Cl2 / 0.25 h / 20 °C 2: lithium hydroxide / tetrahydrofuran; H2O / 0.5 h / 5 °C 3: hydrogen / Pd/C / methanol View Scheme |
(S)-3-[3-(benzyloxycarbonyl)-5-oxooxazolidin-4-yl]propanoic acid
L-glutamic acid 5-(4-nitroanilide)
Conditions | Yield |
---|---|
Multi-step reaction with 4 steps 1: thionyl chloride / CH2Cl2 / 0.5 h / Heating 2: pyridine / CH2Cl2 / 0.25 h / 20 °C 3: lithium hydroxide / tetrahydrofuran; H2O / 0.5 h / 5 °C 4: hydrogen / Pd/C / methanol View Scheme |
N-tert-butoxycarbonyl glutamic acid tert-butyl ester
4-nitro-aniline
L-glutamic acid 5-(4-nitroanilide)
Conditions | Yield |
---|---|
Multi-step reaction with 2 steps 1: HATU; N-ethyl-N,N-diisopropylamine / N,N-dimethyl-formamide / 0.5 h / 120 °C / Microwave irradiation 2: hydrogenchloride / 1,4-dioxane / 4 h / 40 °C View Scheme |
L-glutamic acid 5-(4-nitroanilide)
Conditions | Yield |
---|---|
With hydrogenchloride In 1,4-dioxane at 40℃; for 4h; |
Conditions | Yield |
---|---|
at 37℃; 0.1 M Tris-HCl buffer, γ-glutamyltransferase from mushroom Lentinus edodes; other enzyme; Michaelis constant Km and Vmax determination; | |
With religiosin B at 37℃; pH=8; Kinetics; aq. buffer; Enzymatic reaction; | |
With religiosin C at 37℃; pH=8; Kinetics; Concentration; aq. buffer; Enzymatic reaction; |
glycylglycine
L-glutamic acid 5-(4-nitroanilide)
L-α-glutamyl=>glycyl=>glycine
Conditions | Yield |
---|---|
With HCl buffer; rat kidney γ-glutamyltranspeptidase pH=8.0; Enzyme kinetics; |
L-glutamic acid 5-(4-nitroanilide)
D-glutamic acid γ-p-nitroanilide
Conditions | Yield |
---|---|
With HCl buffer; rat kidney γ-glutamyltranspeptidase pH=8.0; Enzyme kinetics; |
Conditions | Yield |
---|---|
With marine bivalve lysozyme; MOPS buffer; Tapes japonica at 37℃; pH=7.0; Enzyme kinetics; | |
With recombinant Pseudomonas nitroreducens IFO12694 γ-glutamyltranspeptidase at 30℃; for 0.0333333h; pH=10.5; aq. buffer; Enzymatic reaction; | |
With C-terminal hexahistidine-tagged Geobacillus thermodenitrificans NG80-2 γ-glutamyl transpeptidase at 52℃; pH=8; Kinetics; aq. Tris-HCl buffer; Enzymatic reaction; |
Conditions | Yield |
---|---|
With Tris-HCl buffer at 37℃; pH=8.0; Enzyme kinetics; Further Variations:; Catalysts; |
glycylglycine
L-glutamic acid 5-(4-nitroanilide)
A
gamma-Glutamyl-glycyl-glycine
B
4-nitro-aniline
Conditions | Yield |
---|---|
With γ-glutamyltransferase from Bacillus subtilis In aq. phosphate buffer at 22℃; pH=8.5; pH-value; Enzymatic reaction; |
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