319-78-8Relevant articles and documents
Enantioselective hydrogenation of cyclic tetrasubstituted-olefinic dehydroamino acid derivatives
Claverie, Jerome,Tang, Chuyan,Tang, Wenjun,Wan, Feng,Wang, Nan,Zhu, Yuxin
, p. 5546 - 5549 (2021/06/12)
An efficient asymmetric hydrogenation of cyclic tetrasubstituted-olefinic dehydroamino acid derivatives has been achieved with a Rh-ArcPhos catalyst, affording a series of α-acylamino-β-alkyl tetrahydropyranones with two contiguous chiral centers in up to 96% ee and 1000 TON.
Reconstruction of Hyper-Thermostable Ancestral L-Amino Acid Oxidase to Perform Deracemization to D-Amino Acids
Ishida, Chiharu,Miyata, Ryo,Hasebe, Fumihito,Miyata, Azusa,Kumazawa, Shigenori,Ito, Sohei,Nakano, Shogo
, p. 5228 - 5235 (2021/11/05)
L-amino acid oxidases (LAAOs) with broad substrate specificity can be used in the deracemization of D,L-amino acids (D,L-AAs) to their D-enantiomers. Hyper-thermostable LAAO (HTAncLAAO) was designed through a combination of manual sequence data mining and ancestral sequence reconstruction. Soluble expression of HTAncLAAO (>50 mg/L) can be achieved using an E. coli system. HTAncLAAO, which recognizes seven L-AAs as substrates, exhibits extremely high thermal stability and long-term stability; the t1/2 value was 95 °C and 99 % ee, D-enantiomer). These results suggest that HTAncLAAO is an excellent biocatalyst to perform this deracemization.
Isolation and structure determination of a new cytotoxic peptide, curacozole, from Streptomyces curacoi based on genome mining
Kaweewan, Issara,Komaki, Hisayuki,Hemmi, Hikaru,Hoshino, Kanata,Hosaka, Takeshi,Isokawa, Gouchi,Oyoshi, Takanori,Kodani, Shinya
, p. 1 - 7 (2018/10/20)
Using genome mining, a new cytotoxic peptide named curacozole was isolated from Streptomyces curacoi. Through ESI-MS and NMR analyses, curacozole was determined to be a macrocyclic peptide containing two isoleucine, two thiazole and three oxazole moieties. Curacozole exhibited potent cytotoxic activity against HCT116 and HOS cancer cells. The proposed biosynthetic gene cluster of curacozole was identified and compared with that of the related compound YM-216391.