Application of n-tris (hydroxymethyl) methyl-3-aminopropane sulfonic acid

Details:

Taps, fully known as n-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, is an amphoteric buffer widely used in the fields of Biochemistry and molecular biology. CAS No.: 29915-38-6, pH buffer range 7.7-9.1, soluble in water (25g / 50ml). Next, Desheng will introduce the synthesis, application and instructions for the use of taps. I hope you can have a more comprehensive understanding of taps after reading this article.

Synthesis of biological buffer taps

A synthetic process of taps introduced in this paper uses trimethylolaminomethane (Tris) and 1,3-propanesulfonic acid lactone (1,3-ps) as raw materials to react in alcohol solvent. The operation process is as follows:

1. Weigh the same molar amount of Tris and 1,3-ps, and take an appropriate amount of alcohol solvent into a 250ml three mouth flask with condensation reflux. Under stirring, heat up and reflux;

2. Stop the reaction after a period of time; At room temperature, after the reaction solution is cooled to room temperature, it is filtered under reduced pressure. The taps coarse filter cake is washed with an appropriate amount of absolute ethanol for 2 ~ 3 times. The taps filter cake is taken out and dried in a vacuum drying oven. The filtrate filtered under reduced pressure is recovered for use.

Application of biological buffer taps

1. Taps, as a common buffer system in DNA screening system, can also be used as a buffer component of RNA samples. It is suitable for the study of electron transfer and phosphorylation of chloroplast thin-layer samples

2. Protect oxygenated hemoglobin from oxidation to methemoglobin during freeze-drying

3. It can be used as background electrolyte for protein microanalysis by capillary zone electrophoresis.

4. It can be used as an important pH stabilizer, which is generally mixed with weak acid and its conjugated base to obtain appropriate pH value.

5. It is usually used in some biochemical diagnostic kits, PCR diagnostic kits and DNA / RNA extraction kits

Purchase instructions for biological buffer taps

If the demand is small, you can directly purchase taps ready to use buffer, usually 200ml, 500ml, 1L, etc., and select the corresponding pH range and concentration according to the experimental requirements. It is very convenient to start and use immediately after arrival. If the demand is large, you can directly contact Desheng professional to directly produce and purchase taps buffer powder, which is conducive to long-term storage, It can be prepared at any time when it needs to be used, which makes it more flexible to use.

The quality service of trimethylolmethylaminopropyl sulfonic acid (TAPs) produced and developed by Desheng is guaranteed. The manufacturer can provide stable supply, and can provide one-stop procurement of raw materials to save your procurement time and cost. Desheng has complete qualifications in all aspects of biological buffer taps, including test reports, etc. and independent intellectual property rights of products. It is a manufacturer worthy of your trust.