Application of TAPS Biological Buffer

Details:

TAPS stands for N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid and is a member of Good's buffer family. Its appearance is a white powder, easily soluble in water, it is a zwitterionic buffer widely used in the fields of biochemistry and molecular biology, pH buffer range is 7.7-9.1, pKa is 8.40 at 25℃, often Buffers for separation of dyes in planar chromatography and for purification of enzymes in ion-exchange chromatography.

one. Biological Buffers for Separating Dyes in Chromatography

Image: TAPS for chromatographic separation of dyes
Chromatography is a laboratory technique commonly used to separate and purify proteins. During this process, the separation capacity of the system is directly related to pH changes. For example, if the pH of the mobile phase (solvent) is close to the pKa, small changes in pH can severely affect retention and therefore cause separation. Since retention of ionizable compounds is particularly sensitive to mobile phase pH, buffers should be added to the system to control this variable.

Based on the most relevant literature on chromatography, Desheng researchers found that while Tris and MES are generally considered to be the best options, other buffers include: TAPS has also been used in cation exchange chromatography, anion exchange chromatography, high-efficiency liquid chromatography (HPLC) and other similar techniques.

two. One of the best biological buffers for cell culture
As mentioned above, TAPS is a member of Good's buffer family. One of the most important features of Good's buffers is that they are non-toxic to cells. Therefore, these chemicals are widely used in cell culture to keep the pH of the experiment under control. Due to their special functions, many Good's buffers/biological buffers are considered ideal for cell isolation, cell culture, enzymatic analysis, and many other biochemical applications.

Desheng researchers found that protonated sulfamate compounds directly inhibit connexin channel activity. In Good's pH buffer (MES (4-morpholinoethanesulfonic acid), HEPES and TAPS (3-{[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]-1), This is demonstrated by pH-dependent connexin channel activity.-propanesulfonic acid)), which have a common sulfamate moiety and the absence of pH-dependent channels in pH buffers without the sulfamate moiety ( Activity. Therefore TAPS is more used for dinoflagellate experiments in cell culture medium.

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