Configuration method of mops buffer

Details:

1-morpholine propanesulfonic acid MOPS, CAS 1132-61-2, is an zwitterionic buffer commonly used in biochemistry and molecular biology. Its structure is similar to 2 - (n-morpholine) ethanesulfonic acid (MES). It also belongs to one of the "good" buffers classified by Dr. Norman good and his colleagues in 1966.

The effective pH buffer range of MOPS is 6.5-7.9, pKa=7.2 (25 degrees), and its pKa is closer to physiological pH7.4 than MES (pKa=6.1), so it is more suitable for physiological related buffer, such as for animal cell culture (recommended concentration should be less than 20 mM), RNA modified agarose gel electrophoresis (20 mM concentration is better for formaldehyde gel). Discontinuous polyacrylamide gel electrophoresis and so on. The following is the relevant knowledge of mops buffer configuration.

Desheng mops packaging and powder

1. Raw materials prepared with 10xmops buffer

83.7 g mops; Molecular weight 209.3 g / mol

13.6 g sodium acetate, trihydrate; MW 136.1 g / mol (Note: anhydrous NaAc is only 82 g / mol or 8.2g)

3.7 g EDTA, disodium dihydrate; MW 372.24 g / mol (recheck the MW of the stored salt)

2. Configuration steps of mops buffer

Add 800 ml of nuclease free distilled water; Mixed dissolution;

Adjust to pH 7 with NaOH (prepared in distilled water without nuclease);

Add deionized water to a final volume of 1000 ml.

3. Precautions for mops buffer

Mops buffer can be stored at 2 ~ 8 ℃ for at least 6 months with 0.2 μ M. high pressure sterilization is not recommended. If it is necessary to prepare mops solution without nuclease, the water can be autoclaved before adding powder to dissolve it. Store at room temperature away from light. If the solution is dark (yellow is OK), do not use it. Mops will interfere with the analysis of Folin protein. High pressure sterilization of mops solution containing glucose will lead to partial inactivation.

4. Stability of mops buffer   Contrary to the general view, mops buffer has sufficient thermal stability for high-pressure sterilization. The solution will turn yellow, but this will not affect its buffering capacity. For example: Farrell RNA method, straw colored buffer is good, but do not use darker buffer.

5. Application fields of mops buffer

RNA agarose gel

Nupage electrophoresis

bis-Tris SDS-PAGE

6. Storage method of mops: mops powder can be stored at room temperature for several years. However, once standing, the bulk powder may gather together to form hard blocks, but its chemical properties will not be affected.

Founded in 2005, Desheng has an independent R & D team and many years of experience in mops buffer production. The products are reliable in quality and favorable in price. They can also meet the needs of many industries. Desheng has reached long-term cooperation with many enterprises. If you are interested, you can call for consultation. Desheng welcomes your call.