Electrophoretic buffer mops

Details:

In the electrophoresis experiment, buffer will be used to maintain the necessary condition for the constant pH value of the electrophoresis stability system, because in the electrophoresis experiment, its positive electrode and negative electrode will have electrolytic reaction, the positive electrode will have oxidation reaction and the negative electrode will have reduction reaction. Long time electrophoresis will change the positive electrode into acid and the negative electrode into alkali. The buffer can keep the pH at the two poles of the solution basically unchanged. It also makes the solution have certain conductivity, which is conducive to the migration of DNA molecules.

Many people have thought of biological buffer MOPS 3- morpholine propane sulfonic acid. It belongs to Good 's buffer and has good solubility in water. The buffer range is between 6.5-7.9. It can be used as an electrolyte system for two-dimensional gel electrophoresis with medium intensity electrophoretic (IEF). It can also be used in Northern hybridization as a buffer for RNA isolation and membrane transfer.

10} commonly used in electrophoretic buffer × Mops buffer is prepared as follows:

(1) Weigh 41.8 g mops and dissolve it in about 700ml DEPC treated water;

(2) Adjust the pH value to 7.0 with 2 n NaOH;

(3) Add 20ml of DEPC treated 1m NaOAc and 20ml of 0.5m EDTA (ph8.0) to the solution;

(4) Constant volume to 1 L, with 0.45 μ M filter membrane to remove impurities and store it away from light at room temperature.

In addition to mops buffer, there are many biological buffers used in electrophoresis. They are also different and suitable for all kinds of electrophoresis. In the current market, there are many businesses of biological buffer, and the product specifications and quality are uneven. Desheng is a manufacturer specializing in the production of biological buffers. At present, the biological buffer products produced include Tris, HEPES, taps, mops, caps, bicine, EPPs, PEP and a series of biological buffers. At present, it has been established for more than ten years, has very rich R & D and production experience and product knowledge, and can provide customers with a lot of technical support and after-sales guarantee. If you need anything, please feel free to contact us!