Biological buffer for protein purification

Details:

General principles of protein purification

Protein purification should use the internal similarity and difference between different proteins, use the similarity between various proteins to remove the pollution of non protein substances, and use the difference of each protein to purify the target protein from other proteins. The size, shape, charge, hydrophobicity, solubility and biological activity of each protein will vary. Using these differences, the protein can be extracted from mixtures such as E. coli lysates to obtain recombinant proteins

Replacement of biological buffer

Although changing the buffer can not improve the protein purity, it plays an extremely important role in the protein purification scheme. Different protein purification methods need buffers with different pH and ionic strength. Each step of protein purification will cause the loss of target protein, especially the step of buffer balance. The protein will bind to any surface that it can touch, and the rapid change of shear force, foaming and ionic strength can easily deactivate the protein. MOPS buffer is often used as protein buffer.

MOPS is not only a pH buffer for protein purification and extraction, but also a component of animal, yeast and mammalian cell buffer medium, and also the best buffer for RNA separation by agarose gel electrophoresis. Due to the lack of the ability to form chelates with most metal ions, mops is recommended as a non coordination buffer 5) pipes when preparing solutions containing metal ions

Desheng company is a manufacturer of biological buffer. At present, the mops products produced by Desheng company have stable performance and good use effect. If you need to buy, you are welcome to consult!