3-morpholine propanesulfonic acid mops product details

Details:

The full chemical name of mops is 3-morpholine propanesulfonic acid. It is white crystal powder at room temperature and has good hydrophilicity. 10g mops powder can be completely dissolved in 100ml water at 20 ℃, and the dissolved solution is colorless and transparent. Mops is often prepared into biological buffer to adjust the pH of solution in biochemical reaction, and it is a facultative ionic biological buffer.

The basic information of mops is as follows:

CAS No.: 1132-61-2

Molecular formula: c7h15no4s

Molecular weight: 209.3

Applicable pH range: 6.5-7.9

PKa value (25 ° C): 7.0-7.4

Purity: 99%

Absorbance: less than 0.05

3-morpholine propanesulfonic acid mops

As a common buffer, mops buffer has a wide range of applications. Next, Desheng Xiaobian will elaborate on several applications of mops buffer:

1. As pH is applicable in the range of 6.5-7.9, mops buffer can be used to separate, purify and extract RNA and protein, but can not be used to separate DNA, because it can interact with DNA and form complexes;

2. Mops buffer can combine with iron, but does not react with most metals such as magnesium, manganese, cobalt and nickel. Mops buffer is often added to prepare cell culture media that do not need iron ions such as bacteria, charcoal yeast and mammalian cells to stabilize the acidity and alkalinity of the solution. It should be noted that the concentration of the buffer has a great impact on the pH of the solution. Therefore, when using mops buffer to adjust the pH value of cell culture, it should be noted that the concentration of mops should be less than 20mm.

3. in the non denaturing agarose gel electrophoresis experiment of RNA, mops buffer is more suitable to stabilize the pH value of the solution than other buffers.

4. Because the UV absorbance of mops buffer is very small, it does not interfere with the measurement of absorption during UV / Vis spectrophotometry,

5. At room temperature, the chemical properties of mops buffer are very stable. When it interacts with the peptide skeleton of bovine serum albumin, it can promote the protein to reach a static stable state. Mops buffer has strong buffering capacity in the pH range of 6.5-7.9. It should be noted that it can change the interaction between lipids, such as affecting the thickness and barrier characteristics of the surface layer of rat endothelium, the mRNA expression form of bovine embryos produced in vitro, and other reactions such as oxidation will occur when encountering strong oxidants such as strong acid and alkali. Therefore, in the application, we should avoid these problems.

Hubei xindesheng Material Technology Co., Ltd. is a professional manufacturer of biological buffer with stable process, which can ensure that the product appearance is pure white crystal powder. Welcome friends to consult, welcome friends to consult.