Cellulase

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SUKACell is an acid cellulase enzymes for texitile obtained from the submerged fermentation of a high cellulase-producing strain                                                ACID CELLULASE  SUKACell INTRODUCTIONAcid Cellulose is an unbranched glucose polymer composed of anhydro-D-glucose units linked by 1,4-b-D-glucoside bonds. These glycosidic bonds can be hydrolysed by cellulolytic enzymes. The native structure of the cellulose is composed of crystalline and less organized amorphous regions.  DESCRIPTIONSUKACell is an acid cellulase enzyme preparation obtained from the submerged fermentation of a high cellulase-producing microbial strain. It is widely used in feed, textile, wastewater treatment and paper making industries. WORKING MECHANISMThe 1,4-b-D-glucosidic linkages in cellulose, lichenin and cereal b-D glucans are hydrolyzed to release free glucose units through the successive action of cellulase enzymes.The less organized amorphous region at the center of the cellulose chain is initially attacked by endoglucanase by random cutting of the b-1,4 glycosidic linkages within the chain thereby producing cello-oligosaccharides. Cellobiohydrolases I and II act on the cello-oligosaccharides from the reducing end and non-reducing end of the cello-oligosaccharides to release cellobiose. b-glucosidase act on the released cellobiose to produce bD glucose units. PROPERTIESSUKACell provides maximal benefit at pH 4.8-5.2 and at the temperature of 55-60 (131 140). SPECIFICATIONSUKACell is available in liquid preparation asSUKACell LQ10 (with enzyme activity of 10,000 U/mL)SUKACell LQ20 (with enzyme activity of 20,000 U/mL) SUKACell is available in solid preparation asSUKACell PW10 (wi1.Usage Textile Auxiliary Agents 2.Apperance white powder 3.Slight fermentation odor 4.PH 5.5-7.0 INTRODUCTION Cellulose is an unbranched glucose polymer composed of anhydro-D-glucose units linked by 1, 4-b-D-glucoside bonds. These glycosidic bonds can be hydrolysed by cellulolytic enzymes. The native structure of the cellulose is composed of crystalline and less organized amorphous regions. SUKACell-CONC is an enzyme preparation obtained from the submerged fermentation of a high cellulase-producing microbial strain. It is widely used in textile and garment stone wash industries.WORKING MECHANISMThe 1, 4-b-D-glucosidic linkages in cellulose, lichenin and cereal b-D glucans are hydrolyzed to release free glucose units through the successive action of cellulase enzymes.The less organized amorphous region at the center of the cellulose chain is initially attacked by endoglucanase by random cutting of the b-1, 4 glycosidic linkages within the chain thereby producing cello-oligosaccharides. Cellobiohydrolases I and II act on the cello-oligosaccharides from the reducing end and non-reducing end of the cello-oligosaccharides to release cellobiose. b- glucosidase act on the released cellobiose to produce b–D glucose units.PROPERTIESSUKACell-CONC will function from a pH of 4.5 to 6.0 with pH 4.8 as an optimum. It will function from 40°C to 60°C with temperature of 55°C as an optimum.Appearance Amber liquid (Note that color does not affect or reflect activity.)Odor Slight fermentation odorpH (as is) 4.5±1.0Density 1.05-1.20ADVANTAGESUKACell-CONC can obviously improve the effect of biofinishing for 100% cotton fabrics, cotton, terylen mixture fabric, both woven and knits, jeans, significantly reduce cottony ball, soften the fabrics and bright the color. Cloth draping property and water absorption are greatly improved. After treatment, jeans feature of clear texture, soft character and old sense. Reduce the dosage of pumice to wash jeans, reducing equipment abrasion and difficulty for wastewater treatment. This product is concentrated product-dilute to use, economical and practical.High activity product is available upon request of customers.ENZYME ACTIVITY UNIT DEFINITIONReducing sugar methodAt a pH of 4. 8 and temperature of 50 °C, to produce the amount of reducing sugar equivalent to 1 mg glucose, it needs l g solid enzyme (or 1mL liquid enzyme) and 1 h hydrolyze sodium carboxymethyl cellulose substrate, as 1 enzyme activity unit, state as u/g (or u/mL). CMCA – DNS for short.EXECUTION STANDARDLight industry standard of the People's Republic of China, QB 2583 2003OPERATION & DOSAGE    OPERATION DOSAGEBiofinishingStonewashingDesize thoroughlyDesize thoroughlyDesize thoroughlyDrain liquor then fill machine to liquor ratio of1 10 (jean/ water)1 10 (jean/ water)Add pumice if required (0-0.5kg / kg of cloths), heat bath to40-60°C50-60°CAdd SUKACell-CONCat the rate of0.25-2.0% owg0.25-0.75% owgAdjust pH to4.5-5.5,with pH 4.8 as an optimum4.5-5.5,with pH 4.8 as an optimumProcess time20-45 minutes(depending on desired effect)30-45 minutes(depending on desired effect)After drainageadd water and softeningadd water and softeningNOTE Some denim fabrics of lower quality may have severe backstaining problems that can be overcome with our ES resist.Use soda ash 1-3 grams and resist ES 1-2 grams (water ratio) to wash out ferment remaining residue, after that adopt our consistent lubricant/ film/ organic silicon oil.The information presented is believed to be accurate. However, said information and products are offered without warranty or guarantee. Sales commitment should pay legal responsibility. We recommend that the prospective user determine the suitability of our materials and suggestions before adopting them on a commercial scale.PACKAGINGSUKACell-CONC is packaged in 25 kg plastic drum.Alternative packaging is available upon request for smaller or larger volumes. STORAGE Store in a cool, dry place and away from direct sunlight. SUKACell-CONC maintains its activity for considerably longer time when stored at lower temperatures (below 20°C). Do not let freeze.HANDLING PRECAUTIONSSUKACell-CONC is non-toxic and biodegradable. However, unnecessary contact with the product should be avoided. Long term exposure to protein based products such as SUKACell-CONC may sensitize certain individuals to the product. Wash hands with warm soapy water after handling. Keep out of reach of children.TECHNICAL SERVICESUKAHAN (Weifang) Bio-Technology Co., Ltd will assist customers with the use of our products in applications development.