SummaryThe recently introduced fluorescent calcium sensitive indicators calcium green, calcium orange and calcium crimson suggest important improvements and advantages to detect small calcium transients at low indicator concentrations. Thermodynamic dissociation constants and dissociation rate c...

The terroir concept can be defined as the effect on food sensory features of the uniqueness of the place, which gives food products a particular taste, mainly due to soil and climate characteristics. The aim of the present study was to understand the relationship between the physiological behavi...

The biosynthesis of the tetrahydropyranyl side chain of verucopeptin (1) in cultures of Actinomadura verrucosospora was investigated. Incorporation experiments using 13C-labeled acetate and propionate, followed by analysis of the labeled metabolite by NMR, revealed that the tetrahydropyranyl sid...

A series of Al-compounds incorporating the bidentate pyrrole-morpholine precursor C4H3NH [2-CH2N(CH2CH2)2O] (1) and aluminum-alkyl have been conveniently synthesized and characterized by 1H and 13C NMR spectroscopy. Reacting one or two equivalents of 1 with AlMe3 in toluene afforded AlMe2{C4H3N ...

Pyrrole and keto-amine based morpholine ligands are conveniently synthesized and their corresponding Al-compounds are afforded accordingly. The combination of 1, {C4H3NH-2-[CHNCH2CH2N(CH2CH2)2O]} and two equivalents of PhNCO generates compound 3, {C4H3N(CONPh)CHN(CONPh)(CH2)2NC4H4O} in relativel...

IntroductionBleomycin pulmonary toxicity (BPT) is a potentially life-threatening consequence of bleomycin usage in patients. An overproduction of epithelium-derived cytokines, habitually linked to allergic inflammation, has been recently revealed in experimental models of BPT.

Cells of Zygosaccharomyces rouxii in a medium containing a high concentration of NaCl were killed during incubation for 2–4 h with a low concentration of a mixture of saponins from tea seeds (TSS). The higher the concentration of NaCl in the medium, the higher the inhibitory effect of TSS on th...

Natural QR (quinine oxidoreductase) inducers play critical roles in cancer chemoprevention. Because tea seed saponins of Camellia sinensis exhibited significant QR inducing activities, this study aimed to find bioactive theasaponin derivatives by structure modification on the sugar part and acyl...

Forty Holstein heifers entered the 12-wk study at approximately 12 wk of age. At enrollment, heifers were blocked by birth date and assigned to 1 of 4 treatments: (1) carrier (30 g; control); (2) lasalocid + carrier (1 mg/kg of body weight; L); (3) chlortetracycline + carrier (22 mg/kg of body w...

A natural antibiotic – Lasalocid is able to form stable complexes with ammonia and organic amines. New complexes of lasalocid with benzylamine and ammonia were obtained in the crystal forms and studied using X-ray, FT-IR, 1H NMR, 13C NMR and DFT methods. These studies have shown that in both co...

Lasalocid is a veterinary ionophore antibiotic used for prevention and treatment of coccidiosis in poultry. It enters the environment with the use of contaminated manure on agricultural land. Despite its extensive use, the effects of lasalocid on non-target soil organisms are poorly explored. We...

The molecular structure of 1:1 complex formed between the naturally occurring polyether ionophore, called lasalocid acid (LAS) and propargylamine (PROP) is studied by X-ray, FT-IR, 1H NMR, 13C NMR and ESI-MS methods. The complex formed between deprotonated LAS acid and protonated PROP molecule i...

The solubilities of lasalocid sodium in five organic solvents including methanol, ethanol, propan-2-ol, ethyl acetate and acetone were determined at temperatures ranging from 283.15 K to 323.15 K at atmospheric pressure using a gravimetric method and three novel polymorphic forms of lasaolocid s...

Lasalocid is an antibiotic from the group of carboxylic ionophores, produced by Streptomyces lasaliensis. But there was limited information of lasalocid on human prostate cancer cells. In the present studies, to better understand its effect in human prostate cancer cells, apoptosis and autophagy...

Lasalocid is a veterinary ionophore antibiotic used for prevention and treatment of coccidiosis in poultry. It is excreted from the treated animals mostly in its active form and enters the environment with the use of contaminated manure on agricultural land. To properly assess the risk that lasa...

ObjectiveTo investigate the effects of ophiopogonin D on human breast cancer MCF-7 cells.

Excessive reactive oxygen species (ROS) play an important role in the development of osteoporosis. Ophiopogonin D (OP-D), isolated from the traditional Chinese herbal agent Radix Ophiopogon japonicus, is a potent anti-oxidative agent. We hypothesized that OP-D demonstrates anti-osteoporosis effe...

In the present study, we investigated whether aqueous extract of Liriope Tuber, ophiopogonin D and spicatoside A derived from Liriope Tuber affect basal or phorbol ester (phorbol 12-myristate 13-acetate, PMA)-induced airway mucin production and secretion from airway epithelial cells. Confluent N...

As an ingredient of vaccines, adjuvants are indispensable for enhancing and directly inducing robust and extensive adaptive immune responses associated with vaccine antigens. In this study, we initially determined that a new molecular immunopotentiator, ophiopogonin D (OP-D), enhanced the antibo...

The measurements of the surface tension of the aqueous solution of n-octyl-β-d-glucopyranoside (OGP) and sodium dodecylsulfate (SDDS) mixtures were performed. From the obtained results the composition and density of the mixed surface layer, standard Gibbs free energy of adsorption and Gibbs fre...