Spectrophotometric, atomic absorption spectrometric and high performance liquid chromatographic (HPLC) procedures have been developed for the determination of betahistine hydrochloride and captopril. The three procedures are based on the reaction of the drugs with carbon disulphide in alkaline m...

Transdermal delivery of betahistine hydrochloride encapsulated in various ethyl oleate, Capryol 90®, Transcutol® and water microemulsion formulations was studied. Two different kinds of phase diagrams were constructed for the investigated microemulsion system. Pseudoplastic flow that is preferab...

A sensitive electroanalytical method for the quantification of betahistine hydrochloride in pharmaceutical formulation has been developed on the basis of an enhanced electrochemical response at glassy carbon electrode modified with polyaniline–zinc oxide (PANI–ZnO). Results suggest that betahi...

Orodispersible film (ODF) is a widely used oral solid dosage form. However, it’s not suitable for drugs with short half-life, bitterness and strong hygroscopicity. The present study aims to develop a sustained release and stable betahistine hydrochloride ODF without bitterness. Drug–resin comp...

In this work, we described an easy preparation of substituted 3-amino-2-nitrothiophenes and selenophenes. Substituted β-chloroacrylonitriles were reacted with sodium sulfide or sodium selenide and bromonitromethane to yield the expected compounds in a one-pot three-step procedure in good yields.

To investigate the affinity of glucocorticoid (GC) to its receptor, the binding of [3H]hydrocortisone 17-butyrate 21-propionate ([3H]HBP) and [3H]dexamethasone ([3H]DEX) in rat liver was analyzed kinetically. Scatchard analysis of [3H]hydrocortisone ([3H]HC) binding yielded a curvilinear plot wi...

Patients who undergo allogeneic stem cell transplantation frequently develop an immunologic disease caused by the reactivation of the graft to the host tissues. This disease is called graft-versus-host disease (GVHD) and it is usually a systemic disorder. In a large proportion of cases, oral dis...

Five experiments were performed to evaluate the effects of dexamethasone (DEX), gender, and testosterone on plasma leptin concentrations in horses. In experiment 1, plasma leptin, insulin, glucose, and IGF-1 concentrations were increased (P  0.1) in plasma leptin response in mares following four...

Glucocorticoids (GCs) have been extensively used as the mainstream treatment for chronic inflammatory disorders. The persistent use of steroids in the past decades and the association with secondary infections warrants for detailed investigation into their effects on the innate immune system and...

Chronic obstructive pulmonary disease (COPD) is a smoking related inflammatory airway disease in which macrophages play a key role. Previously we have shown that cigarette smoke extract (CSE) causes suppression of macrophage inflammatory mediators, with the exception of IL-8. We now investigate ...

A sensitive radioimmunoassay for dexamethasone 17,21-dipropionate and its four metabolites in human plasma and urine has been developed using single anti-dexamethasone antiserum. The antiserum was obtained by immunizing rabbits with dexamethasone-3-oxime-bovine serum albumin conjugate. All of th...

We have optimized a formulation of a prodrug of dexamethasone (DXM), dexamethasone palmitate (DXP) for pulmonary delivery as a dry powder. Formulations were prepared by spray drying DXP with 1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine (DPPC) and Hyaluronic Acid (HA) as excipients. Large porous p...

Basolateral membrane K+ channels play a key role in basal and agonist stimulated Cl- transport across airway epithelial cells by generating a favourable electrical driving force for Cl- efflux. The K+ channel sub-types and molecular mechanisms of regulation by hormones and secretagoues are still...

Oral lichen planus (OLP) and recurrent aphthous stomatitis (RAS) are chronic inflammatory conditions often characterised by erosive and/or painful oral lesions that have a considerable impact on quality of life. Current treatment often necessitates the use of steroids in the form of mouthwashes,...

Linoleic and oleic acid anilides profoundly inhibited the production of reactive oxygen metabolites (ROM) in human polymorphonuclear leukocytes (PMNL) induced by a tumor promoter, phorbol myristate acetate (PMA). The addition of a Ca2+ ionophore, A23187, or a chemotactic peptide, formyl-methiony...

Previous empirical energy calculations of the conformations of acetyl-l—phenylalanine p-acetyl anilide were revised using molecular mechanics model (MM2) with full geometry optimization. Except for finding additional local minima with respect to side-chain rotations, the results confirmed the p...

Erucic acid (C22:1, Δ13Z) is an interesting building block for the oleochemical industry and is abundantly present in Crambe abyssinica seed oil (∼59% of C22:1). For the enrichment of fatty acids (FA), lipases can be employed and we have found in a previous protein engineering study suitable v...

Growing and resting cells of several yeasts, which catalyze the hydride transfer to a carbonyl, were screened and used in conditions to find out the suitable methodology to prepare clofibrate analogues. Clofibrate is an antilipidemic drug. In particular, the bioreduction of ethyl 2-(4-chlorophen...

The present communication aims to develop bisazo disperse dyes based on 3-[4-(4-amino-2-chlorophenoxy)anilino]phenol (DAP) both as a coupling component as well as a diazonium salt. Coupling reaction of DAP was carried out with a diazonium salt of 4-aminoacetanilide to yield a monoazo disperse dy...

Novel push-pull fluorescent dyes – 7-(diethylamino)furo- and 7-(diethylamino)thieno[3,2-c]coumarins derivatives have been synthesized using formyl derivatives of furo- and thieno[3,2-c]coumarins as starting materials. Electron absorption and fluorescent spectra of the dyes have been recorded in...