Agar Listeria according to Ottaviani and Agosti (ALOA) is the mandatory medium used for the detection and enumeration of Listeria monocytogenes in foods according to the official International Organization for Standardization (ISO) methods. On ALOA, Listeria spp. appear as bluish-green colonies ...

The carcinostatic activity of a 4-hydroxy 2,3-trans-pent-en-1-al(HPE): cysteine adduct was studied against the ascitic form of Sarcoma 180 both in vitro and in vivo. The effect of HPE treatment in vitro (at concentrations in excess of 0·16 mM) was to depress primarily the incorporation of 3H-th...

1α,25-Dihydroxyvitamin D3 exerts its actions by binding to vitamin D receptor (VDR). We are continuing the study related to the alteration of pocket structure of VDR by 22-alkyl substituent of ligands and the relationships between the alteration and agonistic/antagonistic activity. Previously w...

Mixtures of equal amounts of the erythro and the threo forms of the β-O-4 lignin model compound 1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)-1,3-propanediol were oxidized with different ligninolytic enzymes and non-enzymic oxidants. The oxidants included cerium(IV) ammonium nitrate (CAN), Fento...

Functionalized and sterically encumbered diaryl ethers were prepared by [3+3] cyclization of 1,3-bis(silyl enol ethers) with 2-aryloxy-3-(silyloxy)alk-2-en-1-ones.

The erythro form of the lignin model compound 1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)-1,3-propanediol (1) was oxidized with laccase/ABTS, lead(IV) tetraacetate (LTA), lignin peroxidase/H2O2, cerium(IV) ammonium nitrate (CAN) and Fenton's reagent. The product profiles obtained with the ...

The sigma-1 receptor (σ1R) is a unique intracellular protein. σ1R plays a major role in various pathological conditions in the central nervous system (CNS), implicated in several neuropsychiatric disorders. Imaging of σ1R in the brain using positron emission tomography (PET) could serve as a ...

SummaryFifteen 1-aryl-3-(3-nitro-4'-methoxyphenyl)-5-phenyl formazans and five 3-aryl-5-(3'nitro-4'-methoxy phenyl)-2-phenyl tetrazolium bromides have been tested against Escherichia coli and Pseudomonas aeruginosa for their antibacterial and against Aspergillus flavus and Helmint...

A number of new crown-formazans with 14 and 15 membered rings have been synthesized, characterized and investigated as selective spectrophotometric chelating agents for lithium. The effect of sodium ion concentration on the background lithium signal was studied. A comparative study for the sensi...

The 1-aza-2-azoniaallene salts 3 react with 3-cyanoformazans 4–6 to give the corresponding 5-(1,5-diarylformazan-3-yl)triazolium salts 7–9 or the rearrangement derivatives 10–12 or the oxidation products 13–15. Reaction of 3a,d with the crown cyanoformazan 18 gave the corresponding crown-for...

Trifluoromethylated alcohols, 2,5-bis(trifluoromethyl)hexane-2,5-diol (1), 1,1,1,4,4,4-hexafluoro-2-(3,4-methylenedioxyphenyl)butane 2,3-diol (2), and 1,1,1-trifluoro-2-oxo-3-(3,4-methylenedioxyphenyl)propane-2-ol (3) were prepared by direct nucleophilic trifluoromethylation of the corresponding...

The red photoluminescence (λmax = 580±20 nm) observed in the synthesis of the Ph3C Gomberg radical is assigned to a luminescence of the (Ph3C–Ph–ĊPh2)* radical dimer.

Upon treatment with the superbasic 1 : 1 mixture of butyllithium and potassium tert-butoxide cis- and trans-1,1,1-triphenyl-2-butene as well as 4,4,4-triphenyl-1-butene undergo a hydrogen/metal exchange to afford 1-(triphenylmethyl)allyl potassium [4,4,4-triphenyl-2-butenyl potassium] which can ...

The electrode reaction mechanism of triphenylmethyl p-nitropheyl sulphide has been investigated by cyclic voltammetry, controlled potential electrolysis and EPR spectrometry. The overall reduction process appears to involve three steps. The anion radical resulting from the first electron uptake ...

The homogeneous and electrode electron transfer to triphenylmethyl phenyl sulphide was analyzed as a function of the reaction Gibbs energy. Several electrogenerated aromatic anion radicals, characterized by increasing standard potentials, were used as homogeneous electron transfer reagents. This...

Hen egg white lysozyme (HEL), an antibacterial enzyme, is a prototype protein for studying the physical and chemical events that underlie the formation of amyloid fibril aggregates. Here, we studied alterations in enzymatic activity and aggregation provoked by oxidation of HEL by hypochlorous ac...

The mixture of squid ink tyrosinase (SIT) at 300 and 500 U/g protein and tannic acid (TA) at 0.5 and 1% (based on protein) increased the turbidity and surface hydrophobicity of natural actomyosin (NAM) from sardine (Sardinella albella) in a dose dependent manner. Total sulphydryl group content d...

A comprehensive and critical compendium of the pKa values in methanol of phenol derivatives, carboxylic acids, both aliphatic and aromatic acid derivatives, protonated amines and nitrogen-protonated heterocyclic bases is given in this paper. Values have been correlated with those obtained in aqu...

The effects of rosmarinic acid (RA) (12, 60 and 300 μM/g protein) on the textural properties and stability of oxidized myofibrillar protein (MP) emulsion gels were investigated. A low dose (12 µM/g) of RA significantly prevented the loss of thiol and ε-NH2 groups and the unfolding of the oxid...

The optimized geometry, 1H and 13C NMR chemical shifts, conformational and natural bond orbital (NBO) analyses, thermodynamic parameters, molecular surfaces, Mulliken, NBO and APT charges for 5-(2-Chloroethyl)-2,4-dichloro-6-methylpyrimidine [C7H7Cl3N2] were investigated by the ab initio HF and ...