2′,3′-O-(2,4,6-Trinitrophenyl) adenosine 5′-triphosphate (TNP-ATP) is a fluorescent analogue of ATP. MgTNP-ATP was found to be an allosteric activator of pyruvate carboxylase that exhibits competition with acetyl CoA in activating the enzyme. There is no evidence that MgTNP-ATP binds to the M...

Novel fluorescent styryl push–pull compounds having electron donating thiazole unit were synthesized by condensing 4-chloro-2-(morpholin-4-yl)-1,3-thiazole-5-carbaldehyde with active methylene compounds via classical Knoevenagel condensation. The synthesized styryl molecules were characterized ...

In captivity, cheetahs (Acinonyx jubatus) frequently suffer from several unusual chronic diseases that rarely occur in their free-ranging counterparts. In order to develop a better understanding of their metabolism and health we documented the urine organic acids of 41 apparently healthy captive...

The Zn2+-dependent deacetylase LpxC is an essential enzyme in Gram-negative bacteria, which has been validated as antibacterial drug target. Herein we report the chiral-pool synthesis of novel d- and l-proline-derived 3,4-dihydroxypyrrolidine hydroxamates and compare their antibacterial and LpxC...

The possibilities of isocratic separation of 2,4-dinitrophenyl derivatives of 12 amino acids that considerably differ in hydrophobicity by micellar mobile phases with different organic modifiers have been discussed. For the first time aliphatic carboxylic acids have been used as modifiers of mic...

Another method has been developed for the separation of amino acids (1 min derivatization plus 22 min separation) by micellar electrokinetic capillary chromatography (MECC) with laser-induced fluorescence detection. Interestingly enough, such work has never been performed on essential amino acid...

A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm I.D.). The separation ...

Fluorescent and colorimetric pH probe possess many advantages including rapid response time, nondestructive testing, and excellent pH sensitivity. However, they usually cannot be utilized simultaneously in both acidic and basic pH ranges. In this study, a new selective and sensitive fluorescent ...

4-Amino-7-nitro-2,1,3-benzoxadiazole (ANBD) usually serves as a scaffold for developing fluorescent probes. In this paper, however, ANBD has been used as a chromogenic unit to design a new colorimetric probe, 4-(8-quinolyl)amino-7-nitro-2,1,3-benzoxadiazole (1), for rapid and visual detection of...

A new, sensitive method was developed for the determination of the neurotoxin domoic acid (DA) using a reversed phase separation followed by post-column derivatization (PCD) with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and subsequent fluorescence detection. The PCD conditions which invo...

A simple and sensitive kinetic spectrophotometric method for the quantitative analysis of pravastatin sodium (PVS) in pure and pharmaceutical formulations has been described. The method is based on the formation of colored product between PVS and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) ...

The regioselective synthesis of 1-heteroaryl-5-amino-4-phenylpyrazoles 3a–g and 1-heteroaryl-5-amino-3-methyl-4-phenylpyrazoles 3h–n was achieved by the treatment of heteroarylhydrazines 1a–g with α-phenylformylacetonitrile 2a and α-phenylacetylacetonitrile 2b, respectively. The structures ...

A series of new (N-((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)-1,3-diphenyl-1H-pyrazole-4-carboxamide derivatives (8–35) were designed, synthesized and evaluated as CDK1/Cdc2 inhibitors. Biological evaluation assays indicated that compounds 16 and 27 showed the most potent growth inhibitory activi...

Three new Coordination Polymers (CPs), namely [Co2(dpb)(dipe)(H2O)2]n (1), [Zn2(dpb)(dipe)(H2O)2]n (2) and {[Co2(dpb)(bib)2(H2O)]·H2O}n (3), where H4dpb = 1-(3,5-dicarboxy-benzyl)-1H-pyrazole-3,5-dicarboxylic acid, dipe = (E)-1,2-di(pyridin-4-yl)ethene, bib = 1,4-di(1H-imidazol-1-yl)benzene, ha...

[(RR′-admpzp)2Ti(OPri)2] complexes (2a–c), synthesized from reaction of Ti(OPri)3Cl (0.5 equiv) with 1-dialkylamino-3-(3,5-dimethyl-pyrazol-1-yl)-propan-2-ol compounds in the presence of triethylamine (0.5 equiv), are pseudo-octahedral with each RR′-admpzp ligand κ2-O,N(pyrazolyl) coordinate...

The copper(II) complexes CuLCl2 (1) and CuLBr2 (2), with the chelating pyrazolylpyrimidine ligand 4-(3,5-dimethyl-1H-pyrazol-1-yl)-2-methyl-6-phenylpyrimidine (L), have been synthesized. A single crystal X-ray diffraction study revealed that 1 and 2 have molecular mononuclear structures. The mol...

A thulium(III) membrane sensor was made using 2% sodium tetraphenyl borate (NaTPB), 65% dibutylphthalate (DBP), 30% poly(vinyl chloride) (PVC) and 3% 1-ethyl-3-(2,5-dihydro-4-(3,5-dimethyl-1H-pyrazol-4-yl)-5-oxo-1H-pyrazol-3-yl)thiourea (ET) as an ionophore. Conductometric study shows selectivit...

A convergent synthesis of a series of 16 new polysubstituted (5-hydroxy-5-(trifluoromethyl)-4,5-dihydro-1H-pyrazol-1-yl)(quinolin-4-yl)methanones, starting from isatin and alky(aryl/heteroaryl) ketones, is described. The diheteroaryl methanones were achieved at yields of up to 95% by a [3 + 2] c...

A simple synthesis of novel mono-, bis- and poly{6-(1H-pyrazol-4-yl)-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines} is reported. The formation of the target compounds was achieved by the reaction of 2-bromo-1-(5-methyl-1-phenyl-1H-pyrazol-4-yl)ethanone with the appropriate aminotriazolethiol or by t...

Two coordination polymers [Cu(Hpptz)]·H2O (1) and [Co(Hpptz)(OH)]·H2O (2) [Hpptz = 5-(4-((1H-pyrazol-1-yl)methyl)-phenyl)-5H-tetrazole] were hydrothermally synthesized and characterized by elemental analysis, IR, PXRD and TGA. 1 and 2 are featured similar 22-membered M2L2 rings with close meta...