A sensitive and convenient detection system for 2-furancarboxylic acid (2-FA) was developed using a three-channel continuous-flow manifold with chemiluminescence (CL) detection. The CL reaction involves hydrogen peroxide, alkali, alcohol and 2-FA methyl ester. 2-FA is first esterified to methyl ...
Gorgonians respond to insult (damage and disease) by producing sclerites containing a purple pigment as opposed to the normal white sclerites. Raman microscopy is used to study the purple areas of three species of diseased coral, Gorgonia ventalina, Pseudoplexaura porosa, and Eunicea laciniata o...
‘Methyl purple′-β-galactoside (4a + 4b) exhibits a 137 nm color shift (454 → 591 nm), a Km of 0.075 mM and fast enzymatic hydrolysis at pH 7.4.The β-galactoside of ‘methyl purple’ is a new chromogenic substrate that exhibits a 137 nm color shift upon hydrolysis at pH 7.4, a Km of 0.075 mM...
Methyl purple is a recently introduced quinone-imide redox dye that has been shown to be an exceptionally sensitive monitor of photosystem I activity in chloroplasts. This compound has a wide range of potential applications for studies of photosynthetic electron transfer and photosynthetic bioen...
Prior to the 19th century the use of purple dyes for textile coloration was expensive and usually limited to royalty. The discovery of several synthetic purple dyes during the 19th century made the production of purple textiles more affordable and thus more readily available. The identification ...
The effects of ultra-high temperature (UHT) (140 °C, 10 s) and packages on quality and shelf life of baked purple sweet potato nectar (BPSPN) were evaluated in this study. UHT did not change pH, titratable acidity (TA), centrifugal sediment ratio, viscosity, lightness, redness, particle size di...
A water-soluble polysaccharide (WPSPP-1) was isolated from purple sweet potato. The chemical structures of WPSPP-1 were characterized by high performance size-exclusion chromatography, methylation analysis, and 1D (1H, 13C) and 2D NMR spectra. Results showed the molecular weight of WPSPP-1 were ...
Purple wheat contains higher levels of anthocyanins than conventional wheat cultivars. The aim of this work was to produce anthocyanin-rich biscuits from purple wheat, and to characterize the final product. Control biscuits, having the same formulation but obtained from a non-pigmented wheat cul...
Osteoarthritis (OA) is a common degenerative joint disease, which is closely related to cartilage degradation. Anthocyanins, a natural flavonoid pigments, exhibit strong antioxidant and anti-inflammatory properties. However, the effect of anthocyanin on inflammatory response in OA has not been i...
Purple acid phosphatases (PAPs) are metalloenzymes that catalyse the hydrolysis of phosphate esters under acidic conditions. Their active site contains a Fe(III)Fe(II) metal centre in mammals and a Fe(III)Zn(II) or Fe(III)Mn(II) metal centre in plants. In humans, elevated PAP levels in serum str...
The effects of harsh UV radiation (λ = 254 nm) on wool and silk dyed with Hexaplex (Murex) trunculus extract are investigated using HPLC-DAD, colourimetry, SEM and FTIR. Indigotin, 6-bromoindigotin, 6,6′-dibromoindigotin and 6,6′-dibromoindirubin were quantified by an efficient HPLC-DAD metho...
Introduction: To demonstrate feasibility and efficacy of commercially-available fibrin tissue sealants as a hemostatic agent and collecting system sealant during hand-assisted LPN.Methods: 15 patients underwent transperitoneal LPN for enhancing renal masses using a hand-assist device. Monopolar ...
ObjectivesTo describe a novel technique to block the distal ureter, thus preventing spillage of tumor cell bearing urine during laparoscopic pluck nephroureterectomy. Currently, there is no consensus on the appropriate management of distal ureter during laparoscopic nephroureterectomy.
Carmine was fed continuously to groups of 54 males and 54 females at dietary levels providing 50, 150 or 500 mg/kg body weight/day for up to 109 wk. As a control, groups of 90 males and 90 females were fed the basal diet for the same period. The rats were derived from parents fed the same dietar...
PurposeTisseel® is used to control minor bleeding during laparoscopic procedures. The DuploSpray MIS™ spray system allows thin, even application over a larger surface area. We use sprayed Tisseel as the sole agent to control hemorrhage and seal the renal collecting system after severe porcine la...
PurposeThe extraction of a tooth is followed by hard and soft tissue changes that can compromise implant placement. The aim of the present retrospective study was to compare the clinical and radiographic outcomes of different ridge preservation procedures based on the use of leukocyte and platel...
The decolorization and organic content reduction of a synthetic Harris’ Hematoxylin (SHH) solution, which is widely used in biochemical and biomedical laboratories, by the use of heterogeneous and homogeneous photocatalytic methods under artificial irradiation has been studied. In the case of h...
Alginate is a biocompatible polymer commonly used for cell encapsulation and synthesis of biomaterials in pure or modified form. In this study we have developed a fluorescence based method to visualize Hematoxylin and Eosin (H&E) stained alginate (pure/protein modified) by electrophoresing it in...
The older metal–hematoxylin stains stain a broad spectrum of tissue components. Several recently introduced metal–hematoxylin stains are highly selective. This selectivity is usually bought at the price of severe limitations on the choice of fixative. A very dilute (2×10−4M) aluminum hematoxy...
Envenomation is a public health problem in many regions of the world. The only available treatment is the serotherapy that has limited efficiency due to the delay of its administration. The goal of this study is to provide a new and more efficient alternative to this treatment. A comparative stu...
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