Pseudouridine supplier | CasNO.1445-07-4

Name
BETA-PSEUDOURIDINE
EINECS
CAS No. 1445-07-4
Article Data21
CAS DataBase
Density 1.641 g/cm³
Solubility
Melting Point 222 °C
Formula C₉H₁₂N₂O₆
Boiling Point 598.4 °C at 760 mmHg
Molecular Weight 244.204
Flash Point 315.7 °C
Transport Information
Appearance
Safety
Risk Codes
Molecular Structure
Hazard Symbols
Synonyms Uracil,5-b-D-ribofuranosyl- (6CI,7CI,8CI);5-(b-D-Ribofuranosyl)uracil;5-Ribosyluracil;NSC 162405;Pseudouridine;Pseudouridine C;b-D-Pseudouridine;b-Pseudouridine;y-Uridine;
PSA 135.64000
LogP -2.78280

Synthetic route

: 1024616-70-3
5'-O-(tert-butyldiphenylsilyl)-2',3'-O-(isopropylidene)pseudouridine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
With trifluoroacetic acid at 20℃; for 1h;	100%

: C9H13N2O9S(1-)*Na(1+)

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
at 70℃; for 2h; pH=9;	100%

: 28113-58-8
5-(2,3-O-isopropylidene-β-D-ribofuranosyl)uracil

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
With hydrogenchloride In methanol at 20℃; for 5h;	93%

: 631920-69-9
2,4-Di-tert-butoxy-5-[(3aS,4S,6R,6aR)-6-(1-methoxy-1-methyl-ethoxymethyl)-2,2-dimethyl-tetrahydro-furo[3,4-d][1,3]dioxol-4-yl]-pyrimidine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
With acetic acid at 50℃; for 5h;	93%

: 68168-19-4
(2S,3S,4R,5R)-2-(2,4-dimethoxypyrimidin-5-yl)-5-hydroxymethyltetrahydrofuran-3,4-diol

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
With acetic acid; sodium iodide for 0.75h; Inert atmosphere; Reflux;	90%

: 78119-28-5, 78119-29-6
5-((3S,4R,5R)-3,4-Dimethoxy-5-methoxymethyl-tetrahydro-furan-2-yl)-1H-pyrimidine-2,4-dione

A: 1445-07-4
pseudouridine

B: 10017-66-0
α-pseudouridine

Conditions

Conditions	Yield
With boron tribromide In dichloromethane at -78℃; for 144h;	A 53% B n/a

: 78119-26-3, 78119-27-4
5-((3S,4R,5R)-3,4-Bis-benzyloxy-5-benzyloxymethyl-tetrahydro-furan-2-yl)-1H-pyrimidine-2,4-dione

A: 1445-07-4
pseudouridine

B: 10017-66-0
α-pseudouridine

Conditions

Conditions	Yield
With boron trichloride In dichloromethane at -78℃;	A 42% B n/a
With boron trichloride In dichloromethane at -78℃; Yields of byproduct given;

: 64714-46-1
(1S)-tri-O-acetyl-1-(2,4-bis-methylsulfanyl-pyrimidin-5-yl)-D-1,4-anhydro-ribitol

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
(i) MCPBA, (ii) H2O, (iii) NaOMe, MeOH; Multistep reaction;

: 67320-14-3, 67320-15-4
(1Ξ)-O2,O4;O3,O5-(R,R)-dibenzylidene-1-(2,4-bis-benzyloxy-pyrimidin-5-yl)-D-ribitol

A: 20337-08-0, 67375-32-0
(Ξ)-5-ξ-D-ribopyranosyl-pyrimidine-2,4-dione

B: 1445-07-4
pseudouridine

C: 10017-66-0
α-pseudouridine

Conditions

Conditions	Yield
(i) BBr3, THF, (ii) MeOH, CH2Cl2; Multistep reaction;

...Expand

: 78119-22-9, 78119-23-0
(2S,3R,4R)-2,3,5-Tris-benzyloxy-1-(2,4-di-tert-butoxy-pyrimidin-5-yl)-pentane-1,4-diol

A: 1445-07-4
pseudouridine

B: 10017-66-0
α-pseudouridine

Conditions

Conditions	Yield
With boron trichloride In dichloromethane at -78℃; for 18h;

: 215110-22-8
5'-O-(tert-butyldiphenylsilyl)pseudouridine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
With hydrogenchloride In methanol for 4h; Desilylation;

: 162635-53-2
2,3-O-isopropylidene-5-O-(1-methoxy-1-methyl-ethyl)-D-ribono-1,4-lactone

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 4 steps 1: 89 percent / tetrahydrofuran / -78 °C 2: 85 percent / L-Selectride; ZnCl2 / CH2Cl2; tetrahydrofuran; diethyl ether / -78 - 20 °C 3: 70 percent / diisopropyl azodicarboxylate; triphenylphosphine / tetrahydrofuran / 48 h / 4 °C 4: 93 percent / aq. acetic acid / 5 h / 50 °C View Scheme

: 5336-08-3
D-Ribono-1,4-lactone

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 5 steps 1: 94 percent / pyridinium p-toluenesulfonate; sodium sulfate / 1 h / 60 °C 2: 89 percent / tetrahydrofuran / -78 °C 3: 85 percent / L-Selectride; ZnCl2 / CH2Cl2; tetrahydrofuran; diethyl ether / -78 - 20 °C 4: 70 percent / diisopropyl azodicarboxylate; triphenylphosphine / tetrahydrofuran / 48 h / 4 °C 5: 93 percent / aq. acetic acid / 5 h / 50 °C View Scheme

Yield

Multi-step reaction with 5 steps
1: 94 percent / pyridinium p-toluenesulfonate; sodium sulfate / 1 h / 60 °C
2: 89 percent / tetrahydrofuran / -78 °C
3: 85 percent / L-Selectride; ZnCl2 / CH2Cl2; tetrahydrofuran; diethyl ether / -78 - 20 °C
4: 70 percent / diisopropyl azodicarboxylate; triphenylphosphine / tetrahydrofuran / 48 h / 4 °C
5: 93 percent / aq. acetic acid / 5 h / 50 °C
View Scheme

: 4-(2,4-di-tert-butoxy-pyrimidin-5-yl)-6-(1-methoxy-1-methyl-ethoxymethyl)-2,2-dimethyl-tetrahydro-furo[3,4-d][1,3]dioxol-4-ol

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 3 steps 1: 85 percent / L-Selectride; ZnCl2 / CH2Cl2; tetrahydrofuran; diethyl ether / -78 - 20 °C 2: 70 percent / diisopropyl azodicarboxylate; triphenylphosphine / tetrahydrofuran / 48 h / 4 °C 3: 93 percent / aq. acetic acid / 5 h / 50 °C View Scheme

: 631920-68-8
(R)-1-{(4R,5S)-5-[(R)-(2,4-Di-tert-butoxy-pyrimidin-5-yl)-hydroxy-methyl]-2,2-dimethyl-[1,3]dioxolan-4-yl}-2-(1-methoxy-1-methyl-ethoxy)-ethanol

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: 70 percent / diisopropyl azodicarboxylate; triphenylphosphine / tetrahydrofuran / 48 h / 4 °C 2: 93 percent / aq. acetic acid / 5 h / 50 °C View Scheme

: 5-[6-(tert-butyl-diphenyl-silanyloxymethyl)-2,2-dimethyl-tetrahydro-furo[3,4-d][1,3]dioxol-4-yl]-2,4-dimethoxy-pyrimidine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 3 steps 1: CH3COOH / 1 h / Heating 2: NaI / acetic acid / 0.42 h / Heating 3: HCl / methanol / 4 h View Scheme

: 221387-73-1
(2R,3S,4R,5S)-2-(tert-Butyl-diphenyl-silanyloxymethyl)-5-(2,4-dimethoxy-pyrimidin-5-yl)-tetrahydro-furan-3,4-diol

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: NaI / acetic acid / 0.42 h / Heating 2: HCl / methanol / 4 h View Scheme

: 6-(tert-butyl-diphenyl-silanyloxymethyl)-4-(2,4-dimethoxy-pyrimidin-5-yl)-2,2-dimethyl-tetrahydro-furo[3,4-d][1,3]dioxol-4-ol

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 3 steps 1: BF3*Et2O; Et3SiH / CH2Cl2 / 1 h / 4 °C / 20 deg C 2: NaI / acetic acid / 0.42 h / Heating 3: HCl / methanol / 4 h View Scheme

: 2-(2,3-O-isopropylidene-β-D-ribofuranosyl)-2-(dimethylaminomethylene)acetic acid lactone

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: 60 percent / C2H5ONa / ethanol / 5 h / Heating 2: 93 percent / HCl / methanol / 5 h / 20 °C View Scheme

: 32580-00-0
2-(2,4:3,5-di-O-benzylidenepentitol-1-yl)pyridine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: (i) nBuLi, THF, (ii) /BRN= 1260242/ 2: (i) BBr3, THF, (ii) MeOH, CH2Cl2 View Scheme

: 64735-77-9
2-methylsulfanyl-pyrimidin-4-yl tri-O-acetyl-β-D-1-thio-ribofuranoside

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: (i) (UV-irradiation), (ii) /BRN= 969135/ 2: (i) MCPBA, (ii) H2O, (iii) NaOMe, MeOH View Scheme

: 57100-18-2
pseudoisocytidine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
With acetic anhydride In pyridine; ethyl acetate	3.2 g (85%)

: 58-96-8
uridine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
With bacterial pseudouridine synthase TruB In aq. buffer at 37℃; Kinetics; Reagent/catalyst; Enzymatic reaction;

: 2,4-dimethoxy-5-(2,3-dideoxy-3-oxo-β-D-ribofuranosyl)pyrimidine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 6 steps 1: 1H-imidazole / dichloromethane / 0 °C 2: sodium tetrahydroborate / methanol / 1 h / 0 °C 3: triethylamine / dichloromethane / 1 h / 0 - 20 °C 4: 1,8-diazabicyclo[5.4.0]undec-7-ene / acetonitrile / 48 h / 0 - 75 °C 5: 4-methylmorpholine N-oxide; osmium(VIII) oxide / water; acetone / -20 °C 6: sodium iodide; acetic acid / 0.75 h / Inert atmosphere; Reflux View Scheme

Yield

Multi-step reaction with 6 steps
1: 1H-imidazole / dichloromethane / 0 °C
2: sodium tetrahydroborate / methanol / 1 h / 0 °C
3: triethylamine / dichloromethane / 1 h / 0 - 20 °C
4: 1,8-diazabicyclo[5.4.0]undec-7-ene / acetonitrile / 48 h / 0 - 75 °C
5: 4-methylmorpholine N-oxide; osmium(VIII) oxide / water; acetone / -20 °C
6: sodium iodide; acetic acid / 0.75 h / Inert atmosphere; Reflux
View Scheme

: 2,4-dimethoxy-5-(5-O-tert-butyldiphenylsilyl-2,3-dideoxy-3-oxo-β-D-ribofuranosyl)pyrimidine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 5 steps 1: sodium tetrahydroborate / methanol / 1 h / 0 °C 2: triethylamine / dichloromethane / 1 h / 0 - 20 °C 3: 1,8-diazabicyclo[5.4.0]undec-7-ene / acetonitrile / 48 h / 0 - 75 °C 4: 4-methylmorpholine N-oxide; osmium(VIII) oxide / water; acetone / -20 °C 5: sodium iodide; acetic acid / 0.75 h / Inert atmosphere; Reflux View Scheme

: 2,4-dimethoxy-5-(2-deoxy-β-D-ribofuranosyl)pyrimidine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 4 steps 1: triethylamine / dichloromethane / 1 h / 0 - 20 °C 2: 1,8-diazabicyclo[5.4.0]undec-7-ene / acetonitrile / 48 h / 0 - 75 °C 3: 4-methylmorpholine N-oxide; osmium(VIII) oxide / water; acetone / -20 °C 4: sodium iodide; acetic acid / 0.75 h / Inert atmosphere; Reflux View Scheme

: C28H36N2O7SSi

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 3 steps 1: 1,8-diazabicyclo[5.4.0]undec-7-ene / acetonitrile / 48 h / 0 - 75 °C 2: 4-methylmorpholine N-oxide; osmium(VIII) oxide / water; acetone / -20 °C 3: sodium iodide; acetic acid / 0.75 h / Inert atmosphere; Reflux View Scheme

: 2,4-dimethoxy-5-(2,3-didehydro-2,3-dideoxy-β-D-ribofuranosyl)pyrimidine

: 1445-07-4
pseudouridine

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: 4-methylmorpholine N-oxide; osmium(VIII) oxide / water; acetone / -20 °C 2: sodium iodide; acetic acid / 0.75 h / Inert atmosphere; Reflux View Scheme

: 292638-85-8
acrylic acid methyl ester

: 1445-07-4
pseudouridine

: 1178552-88-9
C13H18N2O8

Conditions

Conditions	Yield
In ethanol for 16h; pH=8.5; triethylammoniumbicarbonate buffer;	98%
With formic acid; triethylamine In ethanol; water for 16h; pH=8.5;	98%
With O-(1H-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate In ethanol for 16h;	98%
In ethanol for 16h; Triethylammonium-bicarbonate buffer;	98%

: 69304-37-6
1,3-Dichloro-1,1,3,3-tetraisopropyldisiloxane

: 1445-07-4
pseudouridine

: 80545-49-9
3',5'-O-(1,1,3,3-tetraisopropyl-1,3-disiloxanediyl)pseudouridine

Conditions

Conditions	Yield
With pyridine at 0 - 20℃;	97.8%
In pyridine at 20℃; Cooling with ice;	97.8%
With pyridine at 0 - 20℃;	94%

: 108-24-7
acetic anhydride

: 1445-07-4
pseudouridine

: 24800-34-8
5-(2,3,5-tri-O-acetyl-β-D-ribofuranosyl)pyrimidine-2,4-(1H,3H)-dione

Conditions

Conditions	Yield
With dmap In N,N-dimethyl-formamide at -30℃; for 3h;	95%
With pyridine at 20℃;	92%
With pyridine Ambient temperature;

: 13831-03-3
tert-butyl prop-2-ynoate

: 1445-07-4
pseudouridine

: C16H22N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 24h; Michael Addition; regioselective reaction;	92%

: 18162-48-6
tert-butyldimethylsilyl chloride

: 1445-07-4
pseudouridine

: 5-[5-(tert-butyl-dimethyl-silanyloxymethyl)-3,4-dihydroxy-tetrahydro-furan-2-yl]-1H-pyrimidine-2,4-dione

Conditions

Conditions	Yield
With 1H-imidazole In N,N-dimethyl-formamide	90%

: 922-67-8
propynoic acid methyl ester

: 1445-07-4
pseudouridine

: C13H16N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 24h; Reagent/catalyst; Michael Addition; regioselective reaction;	90%

: 60998-71-2
phenyl prop-2-ynoate

: 1445-07-4
pseudouridine

: C18H18N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 24h; Michael Addition; regioselective reaction;	88%

: 1445-07-4
pseudouridine

: 623-47-2
propynoic acid ethyl ester

: C14H18N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 24h; Michael Addition; regioselective reaction;	87%

: 91805-17-3
naphthalen-2-yl prop-2-ynoate

: 1445-07-4
pseudouridine

: C22H20N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 24h; Michael Addition; regioselective reaction;	86%

: 18937-79-6
methyl 2-hexynoate

: 1445-07-4
pseudouridine

: C16H22N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 36h; Michael Addition; regioselective reaction;	85%

: 111-80-8
methyl non-2-ynoate

: 1445-07-4
pseudouridine

: C19H28N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 36h; Michael Addition; regioselective reaction;	85%

: 107-13-1
acrylonitrile

: 1445-07-4
pseudouridine

A: 1188522-54-4
C15H18N4O6

B: 967-23-7
Monocyanoethylpseudouridin

Conditions

Conditions	Yield
With N-ethyl-N,N-diisopropylamine In N,N-dimethyl-formamide at 45℃; for 24h;	A 9% B 84%

...Expand

: 1445-07-4
pseudouridine

: 2216-94-6
phenylpropynoic acid ethyl ester

: C20H22N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 36h; Michael Addition; regioselective reaction;	84%

: 4341-76-8
Ethyl 2-butynoate

: 1445-07-4
pseudouridine

: C15H20N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 36h; Michael Addition; regioselective reaction;	82%

: 4891-38-7
phenylpropynoic acid methyl ester

: 1445-07-4
pseudouridine

: C19H20N2O8

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 36h; Michael Addition; regioselective reaction;	82%

: 762-42-5
dimethyl acetylenedicarboxylate

: 1445-07-4
pseudouridine

: C15H18N2O10

Conditions

Conditions	Yield
With 1,8-diazabicyclo[5.4.0]undec-7-ene In N,N-dimethyl-formamide at 20℃; for 48h; Michael Addition; regioselective reaction;	77%

: 4637-24-5
N,N-dimethyl-formamide dimethyl acetal

: 1445-07-4
pseudouridine

: 64272-68-0
1,3-dimethyl-Ψ-uridine

Conditions

Conditions	Yield
In dimethyl sulfoxide at 110℃; for 1h;	75.4%
In dimethyl sulfoxide at 110℃; for 1h;	75.4%
Heating;
at 110℃; for 1h;
at 110℃; for 1h;

: 40615-36-9
4,4'-dimethoxytrityl chloride

: 1445-07-4
pseudouridine

: 5'-O-(dimethoxytrityl)-pseudouridine

Conditions

Conditions	Yield
With pyridine for 14h; Ambient temperature;	73%
With pyridine at 20℃; for 2h;	58%

: 76-83-5
trityl chloride

: 1445-07-4
pseudouridine

: 5'-O-tritylpseudouridine

Conditions

Conditions	Yield
With pyridine at 20℃; for 24h;	66%

: 1445-07-4
pseudouridine

: 14470-28-1
mono-4-methoxytrityl chloride

: 5'-O-(monomethoxytrityl)-pseudouridine

Conditions

Conditions	Yield
With pyridine at 20℃; for 15h;	58%

: 5457-29-4
N-(3-iodopropyl)phthalimide

: 1445-07-4
pseudouridine

: 1188522-55-5
C20H21N3O8

Conditions

Conditions	Yield
With potassium carbonate In N,N-dimethyl-formamide at 20℃;	57%

: 5457-29-4
N-(3-iodopropyl)phthalimide

: 1445-07-4
pseudouridine

A: 1188522-55-5
C20H21N3O8

B: 1244969-47-8
C31H30N4O10

Conditions

Conditions	Yield
With potassium carbonate In N,N-dimethyl-formamide at 20 - 50℃;	A 25% B 37%

...Expand

Pseudouridine Specification

The Pseudouridine is an organic compound with the formula C₉H₁₂N₂O₆. The IUPAC name of this chemical is 5-[(2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-pyrimidine-2,4-dione. With the CAS registry number 1445-07-4, it is also named as 2,4(1H,3H)-Pyrimidinedione, 5-β-D-ribofuranosyl-. Besides, it may offer protection from radiation.

Physical properties about Pseudouridine are: (1)ACD/LogP: -0.37; (2)# of Rule of 5 Violations: 1; (3)ACD/LogD (pH 5.5): -0.37; (4)ACD/LogD (pH 7.4): -0.41; (5)ACD/BCF (pH 5.5): 1; (6)ACD/BCF (pH 7.4): 1; (7)ACD/KOC (pH 5.5): 14.94; (8)ACD/KOC (pH 7.4): 13.82; (9)#H bond acceptors: 8; (10)#H bond donors: 5; (11)#Freely Rotating Bonds: 5; (12)Polar Surface Area: 77.54 Å²; (13)Index of Refraction: 1.623; (14)Molar Refractivity: 52.5 cm³; (15)Molar Volume: 148.7 cm³; (16)Polarizability: 20.81×10^-24cm³; (17)Surface Tension: 80.3 dyne/cm; (18)Density: 1.641 g/cm³; (19)Flash Point: 315.7 °C; (20)Enthalpy of Vaporization: 93.71 kJ/mol; (21)Boiling Point: 598.4 °C at 760 mmHg; (22)Vapour Pressure: 3.63E-15 mmHg at 25°C.

You can still convert the following datas into molecular structure:
(1)SMILES: O=C1N\C=C(/C(=O)N1)[C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O
(2)InChI: InChI=1/C9H12N2O6/c12-2-4-5(13)6(14)7(17-4)3-1-10-9(16)11-8(3)15/h1,4-7,12-14H,2H2,(H2,10,11,15,16)/t4-,5-,6-,7+/m1/s1
(3)InChIKey: PTJWIQPHWPFNBW-GBNDHIKLBY
(4)Std. InChI: InChI=1S/C9H12N2O6/c12-2-4-5(13)6(14)7(17-4)3-1-10-9(16)11-8(3)15/h1,4-7,12-14H,2H2,(H2,10,11,15,16)/t4-,5-,6-,7+/m1/s1
(5)Std. InChIKey: PTJWIQPHWPFNBW-GBNDHIKLSA-N

Product Name

BETA-PSEUDOURIDINE

Synthetic route

Pseudouridine Specification

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