• Name

  Thiazolyl Blue

• EINECS 206-069-5
• CAS No. 298-93-1
• Density 1.4886 (estimate)
• Solubility slightly soluble in water
• Melting Point 195 °C (dec.)(lit.)
• Formula C₁₈H₁₆BrN₅S
• Boiling Point
• Molecular Weight 414.329
• Flash Point
• Transport Information
• Appearance yellow crystalline powder
• Safety 22-24/25-53-45
• Risk Codes 46
• Molecular Structure
• Hazard Symbols Xi, T
• Synonyms 2H-Tetrazolium,2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (8CI,9CI);3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (6CI,7CI);(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide);2,5-Diphenyl-3-(4,5-dimethylthiazol-2-yl)-2H-tetrazolium bromide;2-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-2H-tetrazolium bromide;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide;3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide;MMTTetrazolium;MTT;MTT tetrazolium;NSC 60102;Thiazolyl blue (Sigma);Thiazolyl blue tetrazolium bromide;
• PSA 75.72000
• LogP 0.28830

Synthetic route

N-<4,5-dimethyl-thiazol-2-yl>-3,N'''-diphenyl-formazan

N-<4,5-dimethyl-thiazol-2-yl>-3,N'''-diphenyl-formazan

298-93-1

thiazolyl blue

Conditions

Conditions	Yield
With N-Bromosuccinimide; ethyl acetate

7631-95-0

sodium molybdate dihydrate

3316-09-4

1,2-dihydroxy-4-nitrobenzene

298-93-1

thiazolyl blue

[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium][MoO2(4-nitrocatechol(-2H))2]

Conditions

Conditions	Yield
In water mixing of aq. solns. of Na2MoO4*2H2O, 4-nitrocatechol and 3-(4,5-dimethylthiaozol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide in acidic medium (pH2.8 - 4.0); pptn., filtration, washing with cold water, drying at 75-80°C;

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298-93-1

thiazolyl blue

23305-68-2

1-(4,5-dimethyl-2-thiazoyl)-3,5-diphenylformazan

Conditions

Conditions	Yield
With dehydrogenase
With succinate dehydrogenase at 37℃; for 4h;

iron(III) ammonium sulfate dodecahydrate

3316-09-4

1,2-dihydroxy-4-nitrobenzene

298-93-1

thiazolyl blue

Fe(3+)*3C6H3NO4(2-)*3C18H16N5S(1+)

Conditions

Conditions	Yield
With sulfuric acid In water

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ammonium metavanadate

298-93-1

thiazolyl blue

37422-56-3

5-methyl-4-(2-thiazolylazo)resorcinol

2O2V(1+)*2C10H7N3O2S(2-)*2C18H16N5S(1+)

Conditions

Conditions	Yield
In chloroform; water

...Expand

298-93-1

thiazolyl blue

(E,Z)-5-(4,5-dimethylthiazol-2-yl)-1,3-diphenylformazan

Conditions

Conditions	Yield
With mitochondrial reductase Enzymatic reaction;

298-93-1

thiazolyl blue

MTT formazan metabolite

Conditions

Conditions	Yield
With A549 cells for 1h; Enzymatic reaction;
With mitochondrial dehydrogenase Enzymatic reaction;

Thiazolyl Blue Chemical Properties

Thiazolyl Blue Toxicity Data With Reference

Thiazolyl Blue Safety Profile