Heparinase III, from Flavobacterium Heparinum

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0 Metric Ton	Negotiable

0 Metric Ton

Negotiable

Min.Order :0 Metric Ton
Purity: ≥ 98 % by reversed phase HPLC analysis
Payment Terms : T/T

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Heparinase Reagent diagnostic reagents

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Application:Heparinase III is useful in many applications (usually with heparinase I and heparinase II) for example: 1. As a research reagent (glycosaminoglycan degradation). 2. For the preparation of d
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Heparinase III cleaves selectively, via and elimination mechanism, sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme is active only towards heparan sulfate and does not cleave heparin or low molecular weight heparins.

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Properties

Molecular Weight: 70.8 kDa

Isoelectric point: 9.6 – 9.9

pH optimum for activity: 7.3

pH range for activity: 4 – 9

Optimal temperature range: 20 ℃ – 37 ℃

Purity: Greater than/equal to 98% by reversed phase HPLC analysis

Protein Impurities: < 0.1%

Specific Activity: > 200 IU/mg (max. 235 IU/mg)

Concentration: 5 IU/mL

Standard: 0.1 IU, 1.0 IU, or customized standard.

Unit Definition: One international unit (IU) of heparinase III is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from heparan sulfate per minute at 35 ℃ and pH 7.0.

Stability: Expiration of heparinase III is 12 months from manufacturing date frozen at – 20 ℃ in PBS.

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