Chondroitinase B

Min.Order / FOB Price:Get Latest Price

1 Metric Ton	Negotiable

1 Metric Ton

Negotiable

Min.Order :1 Metric Ton
Purity: ≥ 90 % by reversed phase HPLC analysis
Payment Terms : T/T

Keywords

Chondroitinase ABC Reagent diagnostic reagents

Quick Details

Appearance:
Application:Determination of contents of chondroitin sulfates by HPLC Processing animal tissues before further investigations Preparation of chondroitin and hyaluronic acid derived unsaturated disaccharides Pr
PackAge:stored in vials
ProductionCapacity:1000|Metric Ton|Day
Storage:
Transportation:AIR shipping

Superiority:

Properties:

Molecular weight: 55kDa

Isoelectric point: 9.4 – 9.6

pH optimum for activity: 7 – 8

pH range for activity: 5 – 10

Optimal temperature range: 20 ℃ – 37 ℃

Purity: ≥ 90% by reversed phase HPLC analysis.

Standard: 1 IU and 5 IU.

Specific Activity: Greater than/equal to 200 IU/mg (substrate: dermatan sulfate). One international unit (IU) of chondroitinase B is defined as the amout of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from dermatan sulfate per minute at 30 ℃ and pH 8.0.

Stability: 12 months froze at– 20 ℃ in aqueous buffers containing sodium phosphate and sucrose 5%.

Details:

Chondroitinase ABC cleaves, via and elimination mechanism, sulfated and non-sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopty at 232 nm. The enzyme is active on chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid.

Substrate Specificity: Chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid.

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