Chondroitinase ABC

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0 Metric Ton	Negotiable

0 Metric Ton

Negotiable

Min.Order :0 Metric Ton
Purity: ≥ 99% by reversed phase HPLC analysis
Payment Terms : T/T

Keywords

Chondroitinase ABC Reagent diagnostic reagents

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Application:Determination of contents of chondroitin sulfates by HPLC Processing animal tissues before further investigations Preparation of chondroitin and hyaluronic acid derived unsaturated disaccharides Pr
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Chondroitinase ABC cleaves, via and elimination mechanism, sulfated and non-sulfated polysaccharide chains containing 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopty at 232 nm. The enzyme is active on chondroitin sulfates A and C, chondroitin sulfates B (dermatan sulfate), chondroitin and hyaluronic acid.

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Properties

Molecular weight: 117.3 kDa

pH optimum for activity: 8.0

Optimal temperature range: 37 °C

Purity: ≥ 99% by reversed phase HPLC analysis.

Specific Activity: ≥ 100 IU/mg (substrate: chondroitin sulfate A).

Standard: 1 IU and 5 IU.

Unit Definition: One international unit (IU) of chondroitinase ABC is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from chondroitin sulfates A per minute at 30 °C and pH 8.0.

Stability: Expiration is at least 12 months from manufacturing date, kept at -20 °C

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