Details:

Recombinant Trypsin Pharmacopoeia grade(Freeze-dried powder)

Recombinant Trypsin 

Specifications：10mg；100mg；1g；packing specifications can be customized according to customer needs

I.Product introduction

Recombinant Trypsin: CAS: 9002-07-7; EC: 3.4.21.4

Recombinant Trypsin is a serine protease, which can specifically cleave the C-terminal peptide end of lysine and arginine.

Trypsin is widely used in various biotechnology processes. For example: proteolysis to produce polypeptides; cell separation of various tissues; proteolysis and sequencing; recombinant insulin production and so on.

II. Product characteristics

Source: Pichia pastoris

Character: white or similar white freeze-dried powder

Purity :(RP-HPLC): β-trypsin≥70%，α-trypsin ≤20%

Specific activity: ≥ 3800 USP units / mg Pro

Host protein residue: ≤0.01%

Host cell DNA residue: ≤ 10 ng/mg

Specificity: cleavage of the carboxy terminal peptide bond between arginine and lysine.

III. Product use

Production of recombinant insulin and analogs; cell culture and fermentation; enzymatic hydrolysis of proteins; cell separation of various tissues.

Iv. Recommend Usage

 It is recommended to use 1mM HCl solution to dissolve, The ration to aimed protein is 1:50 to 1:1000, optimal ph 7.0-9.0, optimal temperature 25-35℃.

Note: The activity is inhibited by serine protease inhibitors such as PMSF, metal ion chelating agents such as EDTA, etc.; high concentration of inorganic salts will have certain effects on enzyme activity.

V. Storage stability

The lyophilized powder can be stored for more than 2 years at temperatures below –15 °C.

Vi. Product advantages

Animal origin free: Recombinant production, no exogenous virus contamination. No animal-derived material is used during production.

Stable quality: stable and continuous batch production can be guaranteed; There is no difference between product batches and the quality is stable.

Lyophilized powder: easy to store and transport.