Details:

Recombinant carboxypeptidase B  80651-95-2

I. Product introduction

Carboxypeptidase B (EC 3.4.17.2) is an exocrine protease belonging to the family of metal carboxypeptidase A and carboxypeptidase B (CPA/CPB). It contains one Zn atom per molecule. It can specifically hydrolyze the peptide bond at the amino terminal of the C-terminal basic amino acids of proteins (Lysine, Arginine, Histidine).

This product is a lyophilized powder which is expressed by recombinant Yeast, freeze-dried after multi-step separation and purification. It is homologous to porcine carboxypeptidase B and also has the same specificity and activity consistent with it. However, the product is free of Animal viruses and other hybrid enzymes and can be used as an alternative to animal-derived carboxypeptidase B for various biotechnological processes.The activity of recombinant carboxypeptidase B was inhibited by typical metal chelating agents such as EDTA, Cu2+, Hg2+, Zn2+, Mn2+ and so on.The optimal pH of CPB is 8.0, and both partial acid and partial base will lead to decreased activity. The activity is close to zero when the pH is close to 5.0, and the activity is completely lost when the pH is close to 12.0.carboxypeptidase B activity increased with the increase of temperature at a certain temperature (<60℃)

II. Product characteristics

Source: Pichia pastoris

Character: White or off-white lyophilized powder

Purity (SDS-PAGE): single main band

Specific activity: ≥100U /mg pro

Specificity: hydrolyzed protein c-terminal basic amino acids (lysine, arginine, histidine)

III. Product use

Recombinant carboxypeptidase B can be used for the production of insulin and analogs, the quality testing of recombinant antibodies, the determination of protein C-terminal amino acids, the production of small molecular proteins or peptides, such as cosmetic peptides, antimicrobial peptides, food flavor peptides, etc. And enzymatic synthesis of certain specific compounds..

Iv. Recommend Usage

It is recommended to dissolve the recombinant carboxypeptidase B lyophilized powder with 25 mM Tris-HCl (pH 7.6) to make the concentration of the enzyme solution 1-10 mg/ml. the amount of enzyme: the target protein (mass ratio) = 1:50-1:1000, the reaction pH is 7.5-9.0 and temperature is 25-35°C.

V. Storage stability

Store at -15℃~-20℃, stable within 24 months; when Tris-HCl buffer is formulated into an enzyme solution, repeatedly frozen and thawed 5 times, no loss of activity.

Vi. Product advantages

Animal origin free: Recombinant production, no exogenous virus contamination. No animal-derived material is used during production.

Stable quality: stable and continuous batch production can be guaranteed; T here is no difference between product batches and the quality is stable. High purity: no any other contaminated proteases, non- specific cutting sites.

Compliance with regulatory requirements: The production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with the NSF ISO 9001:2015 quality system and in accordance with GMP guidelines.

Complete quality documentation: Relevant regulatory support documents are available upon request.

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