Details:

ecombinant enterokinase 9014-74-8

I. Product introduction

Enterokinase (light chain) is a highly specific protease that recognizes the sequence of ASP ASP ASP Lys and hydrolyzes polypeptides at the C-terminal of Lys. It can transform trypsinogen into trypsin, or it can cleave the fusion protein with this recognition sequence. This product is a high-purity, high-activity bovine enterokinase prepared by recombinant yeast secretion expression system. It has a wide range of applications (4-45 ℃, pH 4.5-9.5), and still has some activities in the presence of various detergent and denaturant.

II. Product characteristics

Source: Pichia pastoris

Character: clear, colorless to pale yellow liquid

Activity: ≥ 5.0U/µl

Purity (SDS-PAGE) : single main band

Specificity: highly specific identification of asp-asp-asp-lys sequence, in the c-end of Lys for enzymatic digestion.

III. Product use

Recombinant enterokinase is used in the development of genetic engineering products, especially in the pharmaceutical industry of biological engineering, genetic engineering, biochemistry and molecular biology.

Iv. Recommend Usage

According to the definition of enzyme activity, examples of enzyme cutting conditions:

20 ~50mM Tris HCI, pH 8.0-8.5 system:

Enzyme: concentration of fusion protein = 0.1-10u: 1mg

The optimum temperature was 25-30 ℃, and the reaction time was 4-24 hours. SDS-PAGE could be used to detect the effect of enzyme cutting.

V. Storage stability

Store below -15℃ for 2 years.

Vi. Product advantages

Animal origin free: Recombinant production, no exogenous virus contamination. No animal-derived material is used during production.

Stable quality: stable and continuous batch production can be guaranteed; T here is no difference between product batches and the quality is stable. High purity: no any other contaminated proteases, non- specific cutting sites.

Compliance with regulatory requirements: The production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with the NSF ISO 9001:2015 quality system and in accordance with GMP guidelines.

Complete quality documentation: Relevant regulatory support documents are available upon request.