The molecular formula for hikizimycin, a new antibiotic isolated from Streptomyces A-5, is now established as C21H37N5O14. Evidence in support of the occurrence of 3-amino-3-deoxy-D-glucose and cytosine residues in the antibiotic molecules is presented. Methanolysis of N,N′-diacetylhikizimycin ...

Anthelmycin inhibits protein synthesis on both pro- and eukaryotic ribosomes by preventing the peptide bond-forming reaction. The drug is structurally similar to certain other 4-aminohexosyl cytosine antibiotics including blasticidin S, gougerotin, amicetin and bamicetin although unlike these co...

Confirmation of the structure of the nucleoside antibiotic hikizimycin was obtained by comparing the 13C NMR chemical shift data of selected model compounds with hikizimycin and its constituents.

Chlamydomonas reinhardii wild strain 6145 c as well as the mutant strain 104, which lacks NAD(P)H-nitrate reductase (EC 1.6.6.2) activity and molybdenum-containing cofactor, exhibit and ammonia-repressible NAD(P)H-cytochrome c reductase activity of identical molecular size, 44 500. This diaphora...

1.1. Three b-type cytochromes (b557.5, b560, and b562.5), plus a chromophore with an absorption peak at 558 nm at 77 °K, have been found to be associated with the electron transport system of bovine heart mitochondria. The reduced minus oxidized spectra of these components at 77 °K, as well as...

Rhodopseudomonas spheroides was grown aerobically in the dark in continuous culture yielding cells with a low bacteriochlorophyll content. The supernatant obtained after cell breakage contained a mixture of cytochromes, apparently of the c-type; these were resolved by spectroscopy at 77°K into ...

1.1. The cytochrome composition of Beneckea natriegens grown under aerobic conditions has been examined.2.2. Cell-free extracts obtained by sonication were separated into particulate and supernatant fractions by centrifugation at 150 000 × g.3.3. The particulate fraction contained cytochromes b...

Comparison of animo acid compositions by the procedure of Cornish-Bowden [Meth. Enzymol. 91, 60–75 (1983)] suggests that eukaryotic ferritins and bacterial cytochromes b557.5, also known as bacterioferritin, have similar amino acid sequences. The comparison test also indicates that eukaryotic c...

Tissue culture cells of virus-transformed and untransformed cell lines had low contents of cytochromes in the respiratory chain, measured per cell or per mg protein of the cells, in comparison to the cytochrome contents of liver cells in vivo.In the virus-transformed cells the contents of cytoch...

Three b-type cytochromes have been recognized in beef heart mitochondria and ETPH. They fractionate into complexes II and III. Complex III contains roughly equimolar amounts of 2 b-type cytochromes and cytochrome c1. The former are functionally and spectrally comparable to bT and bK. Cytochrome ...

1.1. Cycles of oxidation followed by reduction at pH 7.2 have been induced in uncoupled anaerobic mung bean mitochondria treated with succinate and malonate by addition of oxygen-saturated medium. Under the conditions used, cytochromes b557, b553, c549 (corresponding to c1 in mammalian mitochond...

A nonheme-iron-containing cytochrome b557, also known as bacterioferritin, was isolated from Pseudomonas aeruginosa. Gel electrophoresis showed that the protein subunits had a molecular weight of 18 kD and it is suggested that the intact molecule contained 24 subunits. The isolated protein conta...

NADH-nitrate reductase (NR) from barley (Hordeum vulgare L. cv. Steptoe) leaves was purified to specific activity of 19 μmol NO2− produced per min per mg protein with 27% yield by blue A sepharose affinity chromatography. Reduction of NR by NADH resulted in spectral absorption maxima at 422 (γ...

We compared 41 patients who received colistimethate with 41 who received polymyxin B for the treatment of serious infections caused by carbapenem-resistant Acinetobacter spp. and found both polymyxins have similar efficacy and toxicity.

Lung impairment is the most life-threatening factor for cystic fibrosis patients. Indeed, Pseudomonas aeruginosa is the main pathogen in the pulmonary infection of these patients. In this work, we developed sodium colistimethate loaded lipid nanoparticles, namely, solid lipid nanoparticles (SLN)...

Infections of the respiratory tract with Pseudomonas aeruginosa in cystic fibrosis patients are frequently treated with colistimethate sodium (CMS). For the intravenous administration of CMS a disposable elastomeric pump is a convenient option. To date, there are no data available on the chemica...

A rapid and simple reversed-phase high performance liquid chromatography (HPLC) method for the quantitation of colistimethate sodium in pharmaceutical formulations has been developed. The chromatographic separation was performed using a Phenomenex Kinetex XB-C18 column with gradient elution usin...

The usefulness of nanotechnology to increase the bioavailability of drugs and decrease their toxicity may be a tool to deal with multiresistant P. aeruginosa (Mr-Pa) respiratory infections. We describe the preparation and the in vivo efficacy and safety of sodium colistimethate-loaded nanostruct...

Starting from a warning of possible artificially high plasma colistin concentrations observed in clinical samples in our recently published LC–MS/MS method, we reviewed our analytical assay and demonstrated, for the first time, that caution must be paid to ionization conditions employed in the ...

We measured the heat capacity of a thin ribbon sample of Zr0.55Al0.10Ni0.05Cu0.30 glassy alloy using a Heat Capacity option of Quantum Design’s Physical Property Measurement System (PPMS) between 1.9 K and 310 K. The cut ribbon pieces were piled up to form a block, where each ribbon layer was a...