Dolichol phosphate is a lipid carrier embedded in the endoplasmic reticulum (ER) membrane essential for the synthesis of N-glycans, GPI-anchors and protein C- and O-mannosylation. The availability of dolichol phosphate on the cytosolic site of the ER is rate-limiting for N-glycosylation. The abu...
A simple and rapid method for determining the activity of dolichol monophosphate phosphatase and dolichol diphosphate phosphatase is reported. This procedure is based on the separation of the substrates, [3H]dolichol monophosphate and [3H]dolichol diphosphate, from their reaction products, [3H]d...
The specificity of the transfer of monosaccharides from sugar nucleotides to dolichol monophosphate catalyzed by liver microsomes was studied. Besides uridine diphosphate glucose, uridine diphosphate-N-acetylglucosamine and guanosine diphosphate mannose were found to act as donors for the format...
Dolichol-P- and dolichol-P-P-linked saccharides were isolated from several trypanosomatid flagellates incubated with [U-14C]glucose. Formation of Glc-P-dolichol and Man-P-dolichol was observed in Herpetomonas muscarum and Leishmania adleri, whereas only the latter derivative was synthesized in T...
Dolichol monophosphate mannose and dolichol monophosphate glucose from mammalian or mold cells were separated by thin-layer chromatography. This separation was not due to a difference in the size of the lipid moieties. It was found that the lipid moieties of dolichol monophosphate mannose and do...
An injectable composite gel was developed from alginic and hyaluronic acid. The enzymatically cross-linked injectable gels were prepared via the oxidative coupling of tyramine modified sodium algiante and sodium hyaluronate in the presence of horse radish peroxidase (HRP) and hydrogen peroxide (...
ObjectiveTo develop a method of freezing small amounts of spermatozoa in polymerized alginic acid drops, which can be liquified after thawing for recovery of the spermatozoa.
Three different ratios of alginic acid/chitosan matrices of ratios 3/1, 1/1 and 1/2, respectively, were crosslinked in their dry state using citric acid (CA)/sodium hypophosphite (SHP) at different conditions controlling the crosslinking process such as citric acid concentration, citric acid/sod...
Calcium alginate nanoparticles as excellent carriers in drug delivery systems were synthesized by controlled gellification method and characterized with Fourier Transform Infrared spectroscopy (FTIR). To compare mean particle size and distribution of calcium alginate nanoparticles with homopolym...
Alginic acid and derivatives were reported for the first time from the bulb of Pancratium maritimum L. The brown algae Sargassum muticum was used as a source of sodium alginate. The alginic acid and calcium alginate production methods were performed. FT-IR spectra of sodium alginate extracted fr...
Alginic acid, a widely used naturally occurring carbohydrate which is generally derived from brown seaweeds, can be considered as a bifunctional heterogeneous and green biopolymeric organocatalyst. Alginic acid, without any post-modification with active Bronsted or Lewis acid centers, was found ...
Phosphorylation of sodium alginate salt (NaAlg) was carried out using H3PO4/P2O5/Et3PO4 followed by acid–base reaction with Ca(OAc)2 to give phosphorylated alginic acid calcium complexes (CaPAlg), as a water dispersible alginic acid derivative. The modified alginate derivatives including phosph...
Alginic acid is a suitable material for modification to prepare new derivatives because of presence of its carboxyl groups. The high content of carboxyl groups over the entire length of its chain renders it an easily modifiable material with a possibility of achieving a high degree of substituti...
This study explores the influence of different synthesis methods and drying conditions in the preparation of sodium alginate-derivate xerogels presenting interesting disintegrant functionalities. Xerogels containing alginic acid (AA) or calcium alginate (CaA) and a mixture of both, AA/CaA, were ...
Increased interest in aesthetic, natural nail coatings has encouraged more in-depth studies, and particularly of ultraviolet curable coatings, their formation, processing, characteristics, and removal. This study investigated the impact of various thixotropic agents (organically modified hectori...
ABSTRACTPurposeWe present long-term observations on photoselective vaporization of the prostate in a prospectively studied cohort of men with obstructive benign prostatic hyperplasia.
The present paper is a new application of inductively coupled plasma atomic emission spectrometry (ICP-AES) to the determination of Na, Cr, Mn, Fe, Co, Ni, Al, Mg, Rh, W, Pt, Ge, Rb, Zr, Nb, Ce, Nd, Eu, Tb, Er and Yb, present as dopants or impurities in single crystals of potassium titanylphosph...
The present article discusses the spectral interferences affecting the determination of Ga, Ho and Tm as dopants in single crystals of potassium titanylphosphate (KTiOPO4) by inductively coupled plasma atomic emission spectrometry (ICP-AES). The Q concept, as proposed by Boumans and Vrakking [Sp...
A flame AAS method is used for the determination of dopants and impurities in potassium titanylphosphate (KTP) single crystals. Sample digestion using sulphuric acid and hydrofluoric acid is proposed as being the most appropriate procedure. The effect of major and minor components in the sample ...
P type copper gallium telluride (CuGaTe2) synthesized from the elements was used as a source for the preparation of films by flash evaporation. Films of different thicknesses were prepared and their electrical conductivity was measured in the temperature range 100–300 K. While in the case of th...
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