Ground state properties of 2,4-diphenyl-1,4-dihydrobenzo[4,5]imidazo[1,2-a]pyrimidine, compound 1, and its derivatives are investigated experimentally and theoretically in Dioxane and DMF. The calculations show that all the studied compounds (1–7) are non-planar, resulting in a significant impa...

We have developed a straightforward approach to diverse synthesis of 2,3-, 2,4-disubstituted pyrimido [1,2-a]benzimidazoles, 2,4,10-trisubstituted 2,10-dihydropyrimido [1,2-a]benzimidazoles and 2,3-disubstituted imidazo [2,1-b]benzothiazoles via multicomponent reactions (MCRs) of heterocyclic az...

Several 2′,3′-dideoxyadenosine analogues with modifications in either the ribose or purine moiety were evaluated for their inhibitory effects on the replication of human immunodeficiency virus (HIV) in MT-4 cell cultures. The 2′,3′-dideoxyriboside of 2,6-diaminopurine (ddDAPR) inhibited HIV ...

The 2′,3′-dideoxyriboside of 2,6-diaminopurine(ddDAPR) is, like 2′,3′-dideoxyadenosine (ddAdo), a potent and selective inhibitor of human immunodeficiency virus (HIV) invitro. The ddDAPR compound inhibits HIV antigen expression and HIV-induced cytopathogenicity in MT4 cells at a 50 % effecti...

3′-Azido-2,6-diaminopurine-2′,3′-dideoxyriboside (AzddDAPR) is a potent and selective inhibitor of human immunodeficiency virus (HIV) replication in vitro. It also inhibits Moloney murine sarcoma virus (MSV)-induced transformation of murine C3H/3T3 embryo fibroblasts. AzddDAPR causes a marked...

A Nickel(II) complex, catena-poly[bis(N-methylthiourea)bis(thiocyanato-κN)nickel(II)], [Ni(Metu)2(NCS)2]n (1) was prepared and its structure was determined by single crystal X-ray analysis. In 1, the nickel(II) ion is bound to four sulfur atoms of bridging Metu ligands and two nitrogen atoms of...

Changes in the levels of amino-acid enantiomers are associated with some serious diseases; consequently, amino acid monitoring in peripheral blood can be used to diagnose and predict the onset of disease. Herein, we report the design and synthesis of a new chiral derivatization reagent, namely s...

BiI3 mediated vicinal azidohydroxylation of α-methylstyrenes 1 with NaN3 in wet DMF affords β-azidoalcohols 4 in good yields. In dry DMF, β-azidoiodides 6 are also obtained by BiI3 mediated vicinal azidoiodination of α-methylstyrenes 1 with NaN3. This present protocol provides the bond forma...

The kinetics and products of the oxidation of four aromatic compounds, i.e. styrene, α-methylstyrene, trans-β-methylstyrene and indene, with Cl atoms were determined at atmospheric pressure and room temperature. Kinetic experiments were carried out in a 400 L Teflon reaction chamber using GC-FI...

Phase equilibria of 3-methylstyrene, 4-methylstyrene, alpha-methylstyrene and a methylstyrene mixture (mainly 3-methylstyrene (60% mol) and 4-methylstyrene (40% mol)) in supercritical CO2 were measured using high-pressure variable-volume view cells based on the static synthetic method. The syste...

Publisher SummaryThis chapter discusses strategies of purifying and characterizing the glycerophosphate acyltransferase. These are (1) cloning of the plsB structural gene encoding the glycerophosphate acyltransferase and construction of strains, which overproduce the enzyme; (2) effective use of...

The oxidation of L-α-glycerophosphate (1) to dihydroxyacetone phosphate (2) is carried out enzymatically under aerobic conditions by L-α-glycerophosphate oxidase coimmobilized with catalase, which serves for the decomposition of the generated hydrogen peroxide. 2 is trapped in an aldolase reac...

Publisher SummaryIn mammalian organs, glycerophosphate acyltranferase is present in both the endoplasmic reticulum (microsomes) and mitochondrial outer membrane. In liver, the glycerophosphate acyltransferase activity is nearly equal in these two organelles, whereas in other organs the microsoma...

The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogs—idebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate ...

Involvement of mammalian mitochondrial glycerophosphate dehydrogenase (mGPDH, EC 1.1.99.5) in reactive oxygen species (ROS) generation was studied in brown adipose tissue mitochondria by different spectroscopic techniques. Spectrofluorometry using ROS-sensitive probes CM-H2DCFDA and Amplex Red w...

In this study, we explored the effect of nanosilver, chitosan characteristics, and solution conditions on gelation temperatures of chitosan/2-glycerophosphate thermosensitive hydrogel. The gelation temperatures of hydrogels with or without 12 ppm nanosilver were insignificantly different regardl...

Mitochondrial respiratory chain enzyme Complexes are present in placenta at proportion similar to other tissues with exception of glycerophosphate dehydrogenase (mGPDH) which is expressed at a very high rate. As shown by Western blot quantification and respiratory chain enzyme activity measureme...

Most malignant cells are highly glycolytic and produce high levels of reactive oxygen species (ROS) compared to normal cells. Mitochondrial glycerophosphate dehydrogenase (mGPDH) participates in the reoxidation of cytosolic NADH by delivering reducing equivalents from this molecule into the elec...

A novel thermosensitive hydrogel consisting of phosphorylated β-cyclodextrin (βCD-PH), β-cyclodextrin (βCD) and chitosan was prepared by embedding βCD and βCD-PH, into the well-studied chitosan/αβ-glycerophosphate system (CS/αβGP). The relevance of this work is the use of βCD-PH to pa...

Malachite is depressed during sulfidization flotation when excess sodium sulfide is added to it. Thus, this study investigated the role and mechanism of ammonium sulfate as a sulfidization promoter for eliminating the depression of excess S2− via micro-flotation experiments, X-ray photoelectron ...