The mica supported Langmuir-Blodgett (LB) monolayers composed of 1,2‑dipalmitoyl‑sn‑glycero‑3‑phosphocholine (DPPC) and 1,2‑dioleoyl‑sn‑glycero‑3‑phosphocholine (DOPC) were used to investigate the effect of cholesterol (Chol) or lauryl gallate (LG) on physicochemical properties of the phospholip...

ObjectiveTo develop silver nanoparticles (AgNPs) using epigallocatechin gallate (EGCG) and evaluate its biocompatibility and inhibition effect on Streptococcus mutans biofilm growth.

Biomaterials based on the modulation of macrophages have gained increased attention recently. Macrophages are generally divided into the pro-inflammatory M1 and pro-regenerative M2 phenotypes. Macrophages play a pivotal role in bone regeneration by regulating osteoblast differentiation and secre...

Propyl gallate (propyl 3,4,5-trihydroxybenzoate, PG) is a phenolic antioxidant that has been used for oil-containing foods to prevent acidification. Owing to its antioxidant properties, PG has been applied to various fields and active research is currently underway to prove PG as an anticancer a...

BackgroundNiemann–Pick disease type C (NPC) is an autosomal recessive inherited disorder with progressive neuronal degeneration. Because conventional diagnostic methods are complicated and invasive, biomarker tests have drawn attention. We aimed to evaluate three urinary conjugated cholesterol ...

The sodium-dependent organic anion transporter SOAT (gene name SLC10A6 in man and Slc10a6 in mice) is a plasma membrane transporter for sulfated steroids, which is highly expressed in germ cells of the testis. SOAT can transport biologically inactive sulfated steroids into specific target cells,...

Methinium colorimetric sensors 1 and 2 were studied for the determination of cholesterol sulfate by UV–Vis spectroscopy in 0.001 M NaCl (H2O:MeOH; 7:3), pH = 7.34. Both of the tested sensors displayed high affinity, excellent selectivity and sensitivity for cholesterol sulfate against other ste...

Steroidal oxime (3β-acetoxy-5α-cholestan- 6-one oxime) has been synthesized using microwave-induced reaction in 3.5 min using saturated steroidal ketone and aqueous hydroxylamine hydrochloride in ethanol. The structure of the compound was elucidated by UV, IR, 1H NMR and X-ray single crystal s...

A high yielding synthesis of leukotriene A4 methyl ester is described which uses D-glucose as starting material

Synthetic cannabinoids, recently used as alternatives to Cannabis sativa, are among the most frequently abused drugs. Identified in 2014, the synthetic cannabinoids N-(1-amino-3-methyl-1-oxobutan-2-yl)-1-(5-fluoropentyl)-1H-indazole-3-carboxamide (5F-AB-PINACA) and methyl [1-(5-fluoropentyl)-1H-...

Fatty acid methyl esters (biodiesel) synthesized by transesterifying vegetable oil and fats with methanol are unsuitable for northern climates and alpine regions. Isopropyl esters derived from oil and isopropanol have superior cold flow properties and lower cloud point but reaction rates are slo...

Cholestanol analyses of sterols have been carried out by removal of the unsaturated sterols by oxidation and extraction followed by gas chromatography of the saturated sterols. The method can detect 0.2% of cholestanol in sterol mixtures.

This paper describes convenient syntheses for labeled and unlabeled cholest-5-en-3-one, cholest-4-en-3-one, epicholesterol, cholest-4-en-3β-ol, and cholest-4-en-3α-ol. The thin-layer chromatography, high-performance liquid chromatography, and gas-liquid chromatography of these compounds and of...

Cerebrotendinous xanthomatosis (CTX) is a lipid storage disease where the basic defect is a lack of the mitochondrial C27-steroid 26-hydroxylase involved in bile acid synthesis (EC 1.14.13.15). Cholestanol and cholesterol accumulate in all tissues. At least part of the accumulation of cholestano...

A cholestanol-enriched diet administered for 8 months to BALB/c mice produced in 20% two kinds of corneal opacities resembling calcific band keratopathy and Schnyder's crystalline dystrophy in humans. The concentrations of cholestanol in serum, liver and cornea of the corneal opacity bearin...

On sonication in water, cholestanol and lysophosphatidylcholine (lysoPC) in 1:1 molar ratio form unilamellar vesicles which are similar to lysoPC/cholesterol vesicles, as judged by gel exclusion chromatography, electron microscopy, and differential thermal scanning. However, the lysoPC/cholestan...

To understand the early steps of C27 brassinosteroid biosynthesis, metabolic experiments were performed with Arabidopsis thaliana and Nicotiana tabacum seedlings, and with cultured Catharanthus roseus cells. [26, 28-2H6]Campestanol, [26-2H3]cholesterol, and [26-2H3]cholestanol were administered ...

The stem-bark of Bleekeria vitiensis A. C, Smith contains traces of the oxindole alkaloid carapanaubine as well as isoreserpiline-ψ-indoxyl. The co-occurrence of these two compounds is of probable biosynthetic significance, representing alternative oxidative rearrangement products of the alkalo...

7α-Hydroxy-cholest-4-en-3-one is a biomarker for bile acid loss, irritable bowel syndrome, and other diseases associated with defective bile acid biosynthesis. Furthermore, 7α-hydroxy-cholest-4-en-3-one is the physiological substrate for cytochrome P450 8B1 (P450 8B1 or CYP8B1), the oxysterol ...

The present study aims to degrade 7-Ketocholesterol (7KC), a major oxysterol implicated in many age-related disorders, through microbial means and find candidate enzymes involved for further application in food systems and as a therapeutic. During initial screening of previously isolated bacteri...