Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an i...

BackgroundThe purpose of this study was to investigate the effects of Crocin on oxidative markers (GPx, SOD, MDA) in animal model of demyelination with Ethidium bromide (EB).

The ribonuclease (RNase) activity of ribonucleases has been assayed by observing the change in fluorescence intensity of ethidium bromide on binding with yeast RNA. The binding of EtBr with RNA was monitored via UV–vis and fluorimetric methods. The degradation of RNA by RNase A was monitored by...

Molecular and cellular mechanisms underlying the peripheral conditioning lesion remain unsolved. We show here that injection of a chemical demyelinating agent, ethidium bromide, into the sciatic nerve induces a similar set of regeneration-associated genes and promotes a 2.7-fold greater extent o...

Single nucleotide polymorphism (SNP) genotyping is an important aspect in understanding genetic variations. Here, we discriminate SNPs using toe-hold mediated displacement reactions. The biological target is an 80 nucleotide long double-stranded–DNA from the mtDNA HV1 region, associated with ma...

The interaction of the classical intercalator ethidium bromide (EtBr) with the double helical network strands of DNA cryogels was investigated. The cryogels were prepared starting from aqueous solutions of DNA (about 2000 base pairs long) at −18 °C using 1,4-butanediol diglycidyl ether crosslin...

The present study was undertaken to develop an effective adsorbent and to study the adsorption of Ethidium bromide and Ethidium monoazide bromide from aqueous solution using the CuO nanoparticles. The characteristics of CuO nanoparticles were determined and found to have a surface area 89.59 m2/...

Quick, simple and reliable assays to screen and understand active efflux pumps operative in pure bacterial cultures or mixed population in environmental samples are important. Although assays are available to monitor efflux activities in pure cultures, none has been used or tested directly on na...

Four kinds of large port zeolites BEA, FAU, LTL and MOR, were used as catalysts in the dehydration of a large molecule, epiandrosterone, to elucidate the effect of their pore structure on its conversion and the selectivity for 5α-androst-2-en-17-one (Δ2-olefin). Silane masking of the zeolite a...

The synthesis of 7β-hydroxy-epiandrosterone (6) possessing strong anti-inflammatory properties was achieved starting from 3β-acetoxy-17,17-(ethylenedioxy)-5-androsten (1). This approach involved as a main step an allylic oxidation of the C-7 followed by two reduction reactions of the double bo...

The effect of long term ingestion of an anabolic steroid, furazabol, was studied on the coagulo-fibrinolytic systems in the rat. During the administration of furazabol at a daily dose of 0.04, 0.2 or 1 mg per rat for 3 months, the most remarkable changes were increase in the plasminogen activato...

Furazabol and 16-hydroxyfurazabol represented the most abundant peaks in urinary metabolic profiles for two men obtained by gas chromatography-mass spectrometry (GC-MS) after oral administration of 5 mg furazabol. The excreted amounts of unchanged furazabol were determined and its response in GC...

The synthesis of podocarpanes, including 12,19-dihydroxy-13-acetyl-8,11,13-podocarpatriene (23), isolated from Gaultheria yunnanensis and not previously synthesized, and totarane-type terpenoids, starting from the natural labdane trans-communic acid (15), is described. Their antiproliferative ac...

Natural product evodiamine and its derivatives represent a promising class of multi-target antitumor agents. However, the clinical development of these compounds has been hampered by a poor understanding of their antitumor mechanisms. To tackle this obstacle, herein, novel fluorescent probes wer...

New aromatic enamines were synthesized by condensation of the commercially available phenoxazine and N-phenyl-1-naphtylamine with 2,2-diphenylacetaldehyde or 2-phenylpropionaldehyde. The materials were examined by various techniques including differential scanning calorimetry, UV and fluorescenc...

2-Hydroxy-6-methylacetophenone, produced in the body of the Australian ponerine ant, Rhytidoponera aciculata (Smith), has been shown by radiolabelling and degradative studies to arise solely from the acetate/malonate pathway.

Using a quality by design approach, a capillary electrophoresis method for the simultaneous determination of dextromepromazine and the oxidation product levomepromazine sulfoxide in levomepromazine was developed. The analytical target profile was defined that the method should be able to quantif...

Derivative spectrophotometry and bivariate calibration algorithm were used for study of run of photooxidation of levomepromazine hydrochloride (LV). The actual concentrations of LV and its main degradation product levomepromazine sulphoxide (LV-SO) were calculated using data provided by applied ...

A high-performance liquid chromatography method for the determination of dextromepromazine, levomepromazine sulfoxide and 2-methoxyphenothiazine in levomepromazine samples was developed. The separation of the analytes was achieved within 10 min on a stationary phase containing cellulose tris(4-m...

ABSTRACTYogurt, a functional dairy food product, is an effective medium for delivering beneficial functional ingredients. One ingredient, γ-aminobutyric acid (GABA), has growing appeal in the development of functional foods for its potential in reducing the risk of diabetes, hypertension, and s...