Trifluorovinyllithium (CF2 = CFLi) has been successfully synthesised from 1,1,1,2-tetrafluoroethane using n-butyllithium at −78°C in ether. Other bases (LDA, DBU, MeLi, t-BuLi, t-BuOK) were tried with varied success. The stability of the system was dependent on temperature, concentration and so...

The effect of acute ammonium acetate intoxication upon orotic acid-6-14C urinary excretion, distribution between blood and liver, and its incorporation into acid-soluble liver nucleotides, was studied in rats.Increased urinary orotic acid excretion was demonstrated following ammonium acetate tre...

Orotic acid, an intermediate in the synthesis of pyrimidine nucleotides, has been shown in the present study to inhibit an early step of purine biosynthesis de novo in cultured human cells as manifested by the decreased synthesis of N-formylglycinamide ribonucleotide (FGAR). Concentrations of or...

Unknown concentrations of orotic acid can be measured by competition with a known amount of [carboxyl-14C] orotic acid for reaction with a limiting amount of phosphoribosylpyrophosphate in the presence of orotate phosphoribosyltransferase and orotidine monophosphate decarboxylase. The dilution o...

In the present study, it was demonstrated that orotic acid, a normal intermediate in pyrimidine synthesis, inhibits purine biosynthesis de novo in normal man. This was associated with depletion of erythrocyte 5-phosphoribosyl-1-pyrophosphate (PP-ribose-P) content. Orotic acid had no inhibitory e...

1.1. [3H]uridine and [3H]orotic acid were equally utilized for labelling of RNA in mouse liver. Incorporation of [3H]cytidine was 2–3 times as high as that of [3H]-labelled uridine or orotic acid.2.2. These results differ from findings in rat liver, where both cytidine and orotic acid are bette...

Bovine milk, the main source of orotic acid in the human diet, contains approximately 80 ng orotic acid per milliliter, .1% of the fat-free solids. Orotic acid is virtually the only component in the acid-soluble nucleotide fraction of bovine milk. Variation is considerable in the concentration o...

We report the effect of the ornithine transcarbamylase (OTC) transgene composed of 1.3 kb of the 5' flanking region of the rat OTC gene fused to rat OTC cDNA on urinary orotic acid excretion in OTC-deficient spf-ash (sparse-fur with abnormal skin and hair) mice during overnight-starvation a...

In rats fed orotic acid, the incorporation in liver subcellular fractions of sugars injected intraperitonealy is altered only for mannose, but not for fucose or galactose. Direct determinations of several glycosyltransferases are done in smooth and rough microsomes: fucosyl-, glactosyl-, N-acety...

Two lysosomal storage diseases, aspartylglucosaminuria and mannosidosis, are associated with highly elevated serum dolichol concentrations. To eludicate possible mechanisms leading to elevated serum dolichols, we studied the effects of Triton WR 1339 (known to increase serum cholesterol) and oro...

Administration of α-1,2,3,4,5,6-hexachlorocyclohexane (α-HCH) to rats decreased the utilization of [2-14C]orotic acid for the synthesis of liver cytidine nucleotides. The specific radioactivities of uridine components of the acid-soluble pool and rRNA increased during the first hours of treatm...

Individuals aboard jet aircraft may be exposed to potentially toxic triaryl organophosphate anti-wear lubricant additives (TAPs) that are converted by cytochromes P450 into toxic metabolites. Consequences of exposure could be reduced by using less toxic TAPs. Our goal was to determine whether an...

In the present study we determined the concentrations and compositions of a suite of isopropylated and tert-butylated triarylphosphate ester (ITP and TBPP) isomers in house dust from the city of Guangzhou located in South China and the city of Carbondale in Midwestern United States. These two gr...

Phosphatase-mediated hydrolysis of organic phosphate may be a viable means of stabilizing heavy metals via precipitation as a metal phosphate in bioremediation applications. We investigated the effect of triethyl phosphate (TEP) on soil microbial-phosphatase activity in a heavy-metal contaminate...

A UPLC-UV method is described for the detection of bDtBPP and related breakdown compounds of Irgafos 168 from single-use bioprocessing bag films using a CSH (charged surface hybrid) fluoro phenyl column and UV detection at 220 nm. The method limits of detection were between 16 μg L‐1 and 60 μ...

α-Amino-α-trifluoromethyl-phenylacetonitrile, PhC(CF3)(CN)NH2, 2, in which the amino group is located on a crowded, chiral, quaternary carbon center, has been studied as a potential reagent for the 19F NMR determination of enantiomeric purity of chiral acids by conversion to their correspondin...

Radicals and radical ions formed on γ-radiolysis of aromatic carboxylic acids containing a thioether group in the polycrystalline state at 77 K and on subsequent warming over the temperature range of 95–293 K were identified using the electron spin resonance technique. In all carboxylic acids ...

Phosphoryl-transfer reactions have long been of interest due to their importance in maintaining numerous cellular functions. A phosphoryl-transfer reaction results in two possible stereochemical outcomes: either retention or inversion of configuration at the transferred phosphorus atom. When the...

The coordination behavior of the heterofunctional phosphine ligand PPh2NH(2-Py), PPh2(o-C6H4)CH=N-(CH2)nPh (n = 0–3) and PPh2(o-C6H4)CH=N-(CH2)n-(2-Py) (n = 1 or 2) towards Rh(I) and Rh(III) are reported and examples of neutral and cationic complexes containing bi-dentate PˆN or tri-dentate PˆN...

The synthesis of rhodium(III) and iridium (III) half sandwich complexes [Cp*M(PEt3) (S-R-S)], M = Rh, Ir; S-R-S = naphthalene-1,8-dithiolate (NaphthS2, a), acenaphthene-5,6-dithiolate (AcenapS2, b) and biphenyl-2,2′-dithiolate (BiphenS2, c) is reported. We also describe the isolation of a new c...