Plants must protect themselves from abiotic stresses such as drought, cold, and high salinity. The common thread of all three stresses is that they cause dehydration, which in turn promotes the formation of reactive oxygen species (ROS). Dehydrin proteins (dehydrins) are a large family of protei...

SummaryAn essential step in pre-mRNA splicing is the release of the mRNA product from the spliceosome. The DEAH box RNA helicase Prp22 catalyzes mRNA release by remodeling contacts within the spliceosome that involve the U5 snRNP. Spliceosome disassembly requires a segment of more than 13 ribonu...

Protein-protein interactions (PPIs) play an important role in cell life activities such as transcriptional regulation, signal transduction and drug signal transduction. The study of PPIs has become a research hotspot in bioinformatics. However, the identification of PPIs using experimental metho...

A long-standing goal in biology is the complete annotation of function and structure on all protein–protein interactions, a large fraction of which is mediated by intrinsically disordered protein regions (IDRs). However, knowledge derived from experimental structures of such protein complexes i...

BackgroundMineral iron(III) recognition by bacteria is considered a matter of debate. The peculiar surface chemistry of novel naked magnetic nanoparticles, called SAMNs (surface active maghemite nanoparticles) characterized by solvent exposed Fe3 + sites on their surface, was exploited for study...

SummaryThe major surface protein of the genus Pseudomonas, OprF, is a non-specific porin that plays an important role in maintenance of cell shape, in growth in a low osmolarity environment, and in adhesion to various supports. The objectives of our study were (i) to carry out a comparative anal...

Pseudomonas aeruginosa antimicrobial resistance is a major therapeutic challenge. DNA vaccination is an attractive approach for antigen-specific immunotherapy against P. aeruginosa. We explored the feasibility of employing Herpes simplex virus type 1 tegument protein, VP22, as a molecular tool t...

BackgroundPseudomonas aeruginosa is a Gram-negative opportunistic bacterium, which considered as a common cause of nosocomial infection and life-threatening complications in immunocompromized and cystic fibrosis patients. Here, we evaluate the protective effect of recombinant vaccines composed o...

Pseudomonas fluorescens 2P24 produces 2,4-diacetylphloroglucinol (2,4-DAPG) as the major antibiotic compound that protects plants from soil-borne diseases. Expression of the 2,4-DAPG biosynthesis enzymes, which are encoded by the phlACBD locus, is under the control of a delicate regulatory netwo...

ObjectiveWith the prolongation of expected lifespan, premature ovarian failure (POF) has gained increased significance as a crucial female health problem. Here we aimed to investigate the diagnostic value of the levels of the growth factor Granulin, which has been associated with epithelial ovar...

Jellyfish grow rapidly and have a strong regenerative ability, indicating that they may express high levels of growth factors. Therefore, the aim of this research was to isolate the growth-promoting components from the jellyfish Cyanea capillata (C. capillata) and to further explore the underlyi...

We have determined the nucleotide (nt) and deduced amino acid (aa) sequences of a unique 1115-kDa. Mycoplasma hyorhinis protein (P 115) with an N-terminal region containing a highly conserved consensus sequence characteristic of nt-binding domains of several ATPase and GTPase enzymes. However, P...

An intramolecular-intermolecular cascade cyclization reaction via multi metal-carbene intermediates has been developed. This reaction uses catalytic amount of Nd(OTf)3, which actives both alkyne and aldehyde moieties. This protocol provides a novel strategy for the synthesis of phenanthro[9,10-b...

An efficient synthesis of a series of 6-chloro-3-substituted-[1,2,4]triazolo[4,3-b]pyridazines is described via intramolecular oxidative cyclization of various 6-chloropyridazin-3-yl hydrazones with iodobenzene diacetate. The structures of the newly synthesized compounds were assigned on the bas...

The quantification of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (Mutagen X or MX) in drinking water is difficult due to the low concentration of MX in drinking water, its high sensitivity to pH change, and matrix effects that interfere with the derivatization and analysis. Typically, ...

We tested the hypothesis that lipopolysaccharide (LPS)-induced myocardial dysfunction is mediated by cyclooxygenase-derived metabolites of arachidonic acid or platelet activating factor (PAF). Ether-anesthetized rats were injected iv with normal saline (NS; 2.5 ml/kg), ibuprofen (cyclooxygenase ...

The production and evaluation of antibodies specific for 5-(2-bromo-E-ethenyl)-2'-deoxyuridine (BVDU) is described. Antibodies were raised against 5-(2-carboxy-E-ethenyl)-2'-deoxyuridine bovine albumin conjugates (CVDU20-BA and CVDU54-BA) and 5'-carboxy-propionyl-BVDU7-BA. The mol...

In rat spinal cord slices, endothelin-1 and endothelin-3 enhanced [3H]inositol phosphate production between 1 nM and 10 μM (endothelin-1 > endothelin-3) while sarafotoxin 6c and the endothelin ETB receptor agonist IRL-1620 c-[Glu9,Ala11,15]endothelin-1-(8–21)) were almost ineffective. BQ-123 (...

The pharmacological profile of N-(6-(2-(5-bromopyrimidine-2-yloxy)ethoxy)-5-(4-methylphenyl)pyrimidin-4-yl)-4-(2-hydroxy-1,1-dimethylethyl) benzensulfonamide sodium salt sesquihydrate (TA-0201), a new antagonist of endothelin receptors, was examined, using human recombinant and prostate endothel...

Endothelin-1-(1–31) is a new bioactive 31-amino-acid-length peptide generated from big endothelin-1 by chymase or other chymotrypsin-type proteases with various pathophysiologic functions. In this study, we have detected the specific and monophasic binding of [125I]endothelin-1-(1–31) in porci...