Partially purified isochorismate synthase (EC 5.4.99.6) from Klebsiella pneumoniae 62-1 was used to produce bulk quantities (3.4–6.8 mg) of isochorismate from chorismate. A new, preparative, low-pressure liquid chromatographic method for the purification of isochorismate was used; a (1.0 × 13....

The in vivo measurement of highly reactive free radicals, such as the OH radical, is very difficult. New specific markers, which are based on the ability of OH to attack the benzene rings of aromatic molecules, are currently under investigation . The produced hydroxylated compounds can be measur...

A rapid and sensitive method for the determination of naproxen and salicylate in serum is presented. The employed strategy combines solid-phase extraction on a reverse-phase membrane with spectrofluorimetry. Solid-phase extraction under optimum pH conditions makes NX to be retained over the soli...

A simple and effective method for the rapid determination of five salicylate and benzophenone-type UV absorbing substances in marketed fish is described. The method involves the use of matrix solid-phase dispersion (MSPD) prior to their determination by on-line silylation gas chromatography tand...

The separation behavior of seven common inorganic anions on an octadecyl-bonded silica (ODS) column coated with cetyltrimethylammonium (CTA+) by phthalate and salicylate eluents was examined with indirect UV detection. The separation became better with increasing anion-exchange capacity for the ...

Thiourea-based bulk optodes were prepared for the determination of salicylate in biological and pharmaceutical samples; these optodes were prepared by embedding the thiourea ionophore in a plasticized poly (vinyl chloride) impregnated with the chromoionophore ETH7075 (Ob) or ETH5294 (Od). The ob...

Preparation of novel salicylate-selective optical sensors (bulk optodes) was performed and applied successfully for salicylate determination in pharmaceutical formulations, Aspirin® and Aspocid®. t-butyl calix[4]arene ionophore was incorporated in a plasticized poly (vinyl-chloride) membrane con...

Salicylate-selective polystyrene micro-optode is engineered using a mixed solvent method. The size of the particles (200–400 nm) and the distribution of the recognition components onto their surface were elucidated by transmission electron microscope and confocal fluorescence microscope. The po...

The lipase-catalyzed chemospecific O-acylation of 3-mercapto-1-propanol and 4-mercapto-1-butanol by several aliphatic carboxylic acid ethyl esters are described. Similar treatment on 1-mercapto-2-propanol and 3-mercapto-2-butanol did not yield the expected O-acyl derivatives.

Electron paramagnetic resonance (EPR) studies of X-irradiated lithium dithionate, Li2S2O6·2H2O, doped with Ni and Rh have shown that these impurities enhance the yield of radicals formed by X-irradiation at room temperature. The signal in the doped samples, measured peak-to-peak of the single E...

It has been established that trace amounts of S2O2-6 anions initiate the appearance of new faces, {111}, on sodium chlorate crystals grown from aqueous solution. The present study compares the growth mechanisms of the {111} habit faces and {111} type faces using laser interferometry techniques. ...

The decomposition of Li2S2O6·2 H2O, Na2S2O6·2 H2O, and MgS2O6·7 H2O has been studied using TG and DTG. Both sodium and lithium dithionates dehydrate in one step. Magnesium dithionate dehydrates in three separate steps. All three of the dithionates undergo further decomposition and lose sulfur...

A process of separation of sodium formate and sodium thiosulfate from sodium dithionate processing industry effluent by selective electrodialysis membrane process was investigated. A mono selective anion exchange membrane (Selemion ASV) and two cation exchange membranes 7000S (CEM) were used to ...

IntroductionEPR-dosimetry using l-α-alanine is an established method for measuring high doses of ionizing radiation. However, since a minimum dose of approximately 4 Gy is required to achieve sufficient low uncertainties (1–2%) for clinical application, alternative dosimeter materials are bein...

Phenol and its derivatives are highly hazardous chemicals profoundly dangerous even at low levels. Hence the management of phenol polluted wastewater represents major environmental challenges. This study investigates the growth and the phenol utilization behavior of microalga DEE 01 isolated fro...

N-[(2-hydroxyl)-propyl-3-trimethyl ammonium] chitosan chloride (HTCC) was synthesized using 1-allyl-3-methylimidazole chloride (AmimCl) as a homogeneous green reaction media. The effects of polymer concentration, temperature, cation valence, anion type and concentration of inorganic salt, and de...

The ionic liquid 1-(triethylene-tetramine)ethyl-3-methyl chloride ([N4MIM]Cl) was first prepared to replace the organic ligand coordinating with CuBr to catalyze atom-transfer radical polymerization (ATRP) in ionic liquid system. [N4MIM]Cl was synthesized via the intermediate IL 1-chloroethyl-3-...

Cobalt(II) and copper(II) complexes with 1-allyl-2-methylimidazole (L), of general formula [ML2(NO3)2], have been prepared in the solid state. The compounds were characterised by structural, spectroscopic and magnetic measurements. The metal ions in both six coordinate complexes are surrounded b...

A simple, green and environmentally benign procedure was developed for the one pot synthesis of 3-methyl-1,4-diphenyl-1,4,5,7-tetrahydro-pyrazolo[3,4-d]pyrimidine-6-one/thiones using catalytic amount of phthalimide-N-sulfonic acid (PISA) in 1-butyl-3-methylimidazolium bromide ([Bmim][Br]) ionic ...

The identification of the cations formed from acenaphthylene is important in the elucidation of the mechanism of its polymerisation. By improved experimental techniques and by MO calculations, some previous assignments of u.v.-vis absorptions to different ions have been corrected and new correla...