Reaction of PdII(tmeda) (tmeda = N,N,N′,N′-tetramethylethylenediamine) with the RNA nucleoside cytidine (Cytd) yields a cyclic nucleoside complex containing three metal entities and three cytidine anions, with metal cross-links involving N(3) and N(4)H− sites. [{Pd(Cytd−-N3, N4)(tmeda)}3](ClO4...

Cytidine 5′-diphospho-1,2-diacyl-sn-glycerol (CDP-diacylglycerol; CDP-DG) is an important intermediate in the biosynthesis of the major glycerophosphate-based phospholipids of prokaryotes and eukaryotes. This compound is expensive to purchase and inefficient to prepare chemically. Radiolabeled ...

The relative incorporation into RNA and DNA of 3H-5-uridine, 3H-cytidine and 3H-6-thymidine was determined in brain, liver, kidney and spleen of the mouse as well as in neoplastic cell cultures. In systems with a high proliferative activity (spleen, cell cultures), the specificity of 3H-5-uridin...

Potentiometric and NMR spectroscopic studies of the nucleotide (NucP)/polyamine (PA) system (where NucP = CDP, CTP, PA = putrescine or spermidine) revealed the formation of molecular complexes (NucP)(Hx+y)(PA) (where Hx+y = number of protons; x - from NucP and y - from PA). Their thermodynamic p...

SummaryRNA splicing is a critical mechanism by which to modify transcriptome, and its dysregulation is the underlying cause of many human diseases. It remains challenging, however, to genetically modulate a splicing event in its native context. Here, we demonstrate that a CRISPR-guided cytidine ...

The synthesis and spectral properties of modified cytidine analogues are described here. To confer fluorescent properties, the cytidine fluorophore was extended by a cinnamyl moiety at the cytidine 5-position, (analogues 1a–c), or extended by a phenyl-imidazolo ring, (analogues 3a–d). Those co...

Novel photolabile sugar derivatives bearing a 4- or 6-O-(o-nitro)benzyl group have been prepared from the corresponding methyl 4,6-O-(o-nitro)benzylidene α-d-glycopyranosides. Regioselective cleavage with BF3·Et2O/Et3SiH led to the methyl 6-O-(o-nitro)benzyl gluco- and manno-α-d-glycopyranosi...

Cholesterol loading of diabetic rats is known to induce marked hyperlipoproteinaemia, and we have reported that enhancement of the activity of intestinal acyl-CoA:cholesterol acyltransferase (ACAT), one of the key enzymes involved in cholesterol absorption, might play an important role in the de...

Employing size-exclusion chromatography, an entropically-controlled separation technique, we have determined the solution conformational entropy (−ΔS) of (1→2)-, (1→3)-, (1→4)-, and (1→6)-linked gluco- and mannobioses with an α anomeric configuration, at quasi-physiological conditions of s...

Supplementation of prebiotic carbohydrates can act as a potent immunomodulator and have the efficacy to induce immune-related genes which are involved in host defense. Pure β-1,4-mannobiose (MNB) showed activation of prophenoloxidase system of shrimp hemocytes in vitro. The resistance of kuruma...

Condensation reaction of 6-formylvisnagin (1) with 1-amino-4,6-dimethyl-2-oxo-1,2-dihydropyridine-3-carbonitrile (2) afforded the novel 1-{[(4-methoxy-5-oxo-5H-furo[3,2-g]chromen-6-yl)methylidene]amino}-4,6-dimethyl-2-oxo-1,2-dihydro-pyridine-3-carbonitrile (MFCMP). The structure of this compoun...

In this work, we have synthesized new coumarin Schiff base molecule, viz., 6-(4-n-heptyloxybenzyoloxy)-2-hydroxybenzylidene)amino)-2H-chromen-2-one and characterized its structural, electronic and spectroscopic properties experimentally and theoretically. The theoretical analysis of UV–visible ...

Osteoporotic bone represents – particularly in case of fractures – difficult conditions for its regeneration. In the present study, the focus was put on a degradable bone substitute material of gelatin-modified calcium and strontium phosphates facing the special demands of osteoporotic bone. T...

A sensitive and specific HPLC method with tandem diode array-fluorescence detection (DAD-FL) has been developed and validated for the simultaneous determination of pheophytin a (phy a) and pyropheophytin a (pyrophy a) in olive oils. Pigments were extracted with reverse phase solid phase extracti...

The diffusion of [125I]-nonoxynol-9 into human semen was investigated in vitro before and after liquefaction. No significant difference was found in the distribution of [125I]-nonoxynol-9 within columns of preliquefied semen, liquefied semen, seminal plasma, or water after ≤120 minutes of diffu...

The absorption and distribution of iodinated derivatives of nonoxynol-9, after vaginal administration in rats, were compared with results reported for [14C] nonoxynol-9. Mono-iodinated nonoxynol-9 was synthesized in addition to the radiolabeled derivative incorporating iodide-125 ([125I]). Six h...

ObjectivesThe Caya® Diaphragm is a newly approved single-size, nonlatex diaphragm. Contragel® is a personal lubricant containing lactic acid approved in Europe and other countries for use with vaginal barrier devices. This study assessed the effectiveness in preventing sperm from penetrating mid...

ObjectivesWe aimed to evaluate the antifertility activity and vaginal irritation effects of tideglusib in vivo using rabbit models and to evaluate the cytotoxical effects of tideglusib to sperm, vaginal cells and vaginal bacteria (L. acidophilus) in vitro.

A new antifungal substance against rice blast fungus (Pyricularia oryzae), 5-(8′Z-heptadecenyl)resorcinol, was isolated from etiolated rice seedlings together with a mixture of its homologues with C13, C15, and C17 saturated alkyl chains. Its structure was determined by 1NMR, 13C-NMR, and MS sp...

Bioassay directed extraction and purification of mango peels revealed the 5-(11′Z-heptadecenyl)-resorcinol (1) and the known 5-(8′Z,11′Z-heptadecadienyl)-resorcinol (2) previously not described in Mangifera indica L. The structures of both compounds were determined by extensive 1D and 2D NMR ...