We have established a highly sensitive high-performance liquid chromatographic method for the determination of an anticancer drug, UCN-01, in human plasma or urine. Using a fluorescence detector set at an excitation wavelength of 310 nm and emission monitored at 410 nm, there was a good linearit...

We determined the cytotoxicity of AG490 as a single agent and in combination with 7-hydroxystaurosporine (UCN-01) in a panel of malignant human glioma cell lines. Because p53 has important roles in cell cycle checkpoints, it has been anticipated that modulation of checkpoint pathways should sens...

UCN-01 is a naturally derived anticancer agent isolated in the culture broth of actinomyces streptomyces. We have developed a sensitive high-performance liquid chromatographic method for the determination of UCN-01 in human plasma. UCN-01 was isolated from human plasma after intravenous administ...

Checkpoint kinase 1 (Chk1) is an evolutionarily conserved serine/threonine kinase that plays an important role in G2/M checkpoint signaling. Here, we evaluate the radiosensitizing effects of a novel selective Chk1 inhibitor MK-8776, comparing its efficacy with a first-generation Chk1 inhibitor U...

Phosphoinositide-dependent protein kinase 1 (PDK1) is a key enzyme, master regulator of cellular proliferation and metabolism; it is considered a key target for pharmacological intervention. Using membranes obtained from DDT1 MF-2 cells, phospho-PDK1 was identified by Western blotting, as two ma...

Purpose7-Hydroxystaurosporine (UCN-01), a Chk1-specific inhibitor, showed promising in vitro and in vivo chemo- or radiosensitizing activity. However, there have been concerns about its limited therapeutic efficacy and risk of side effects. A method of enhancing the treatment efficacy of UCN-01 ...

With the introduction of arsenic trioxide and all-trans retinoic acid, the prognosis of acute promyelocytic leukemia has greatly improved. However, all-trans retinoic acid resistance is still unresolved in acute promyelocytic leukemia relapsed patients. In this study, the clinical achievable con...

Superhydrophobic coating have presented enormous application value in mechanical, architectural and other engineering in decade. However, there are some problems that we have to envisage, such as weak mechanical strength, complex technologies and toxic modifiers, which greatly limits its applica...

We investigated correlation between structure and electron transport properties of composite films synthesized by the ion-beam sputtering of Cu + SiO2 target. Photoluminescence (PL) spectra testify to an oxygen deficiency in the silicon dioxide matrix in agreement with the Raman spectroscopy, wh...

Fragment ions produced from tetraethylorthosilicate (TEOS) in a Freeman-type ion source were investigated using a low-energy mass-selected ion beam system and then, their mass numbers were identified. Although the chemical formulae of these fragments were not completely identified yet, the possi...

This paper discusses the relationships between the deposition parameters and properties of thick (e.g., 5 μm) tetraethylorthosilicate (TEOS) silicon dioxide films deposited on silicon wafers using a dual-frequency plasma-enhanced chemical vapor deposition (PECVD) system. The deposition paramete...

In the process of shape transformation of metal nanoparticles (MNPs) embedded in dielectrics resulting from swift heavy-ion irradiation, key parameters such as the energy deposition threshold, the necessity of a molten track and the NP size have been identified for amorphous silicon dioxide. The...

This paper aims to highlight the effect of SiO2 substrate on the buckling behavior of Zinc Oxide (ZnO) nanotube. ZnO nanotube is modeled in various length and diameter to show the effect of size on buckling loads. Silicon Dioxide (SiO2) is the material on which Zinc Oxide (ZnO) is grown and used...

Graphene layers were synthesized on silica and 90, 300, 400 and 700 nm thick silicon dioxide films by chemical vapor deposition at semi-atmospheric pressure environment, using controlled mixtures of hydrogen, argon and methane gases. The effects of the methane flux and the total pressure on the ...

We investigated the effect of helium ion beam treatment on the etching rate of silicon dioxide in a water based solution of hydrofluoric acid. A 460-nm-thick silicon dioxide film on silicon was irradiated with helium ions having energies of 20 keV and 30 keV with ion fluences ranging from 1014 c...

This study aimed to improve the fluorescence performance of lac dye by preparing carbon quantum dots in an attempt to diversify the applicability of lac dye in fluorescence detection. The highly photoluminescent (PL) ld-CQDs were synthesized for the first time using lac dye as a precursor by a f...

The accumulated stock of coal fly ash becomes more and more huge nowadays, and it could result in severe environmental issues. Therefore, developing a facile and environment-friendly approach to dispose the coal fly ash is highly desirable. Herein, a novel process combining a traditional acid ro...

We report herein an original and rapid synthesis of 2-substituted-4,11-dimethoxy-1-(phenylsulfonyl)-2,3-dihydro-1H-naphtho[2,3-f]indole-5,10-diones by TDAE mediated synthesis of N-benzylsulfonamides followed by an intramolecular N-arylation using Cu-catalyzed system.

VP 4—8as a highly potent behavioral-active metabolite of arginine-vasopressin (VP) has been studied in detail at four levels, i.e. ligand level, membrane binding level, intracellu-lar level and nuclear level. The purpose of this chapter is to review and discuss the main results obtained from ou...

A chiral amine, 1-(2,5-dimethoxyphenyl)ethylamine, was found to be an effective chiral auxiliary for the diastereoselective alkylation of its aldimines with alkylmetals. The 1-(2,5-dimethoxyphenyl)ethyl group of the chiral auxiliary could be removed by the acetylation and then oxidation of the r...