Acetoacetyl-CoA reductase (PhaB), involved in poly(3-hydroxybutyrate) [P(3HB)] biosynthesis, requires the coenzyme NADPH as a reducing agent. In this study, the effect of NADPH supply on P(3HB) production was investigated in vitro and in vivo using a phosphite dehydrogenase double mutant (PtxDEA...

A diastereoselective (de 80–92%) synthesis of α-amino phosphonates was accomplished by reaction of diethyl phosphite sodium salt with 3-R-1-phenyl-2,3-dihydro-1H-naphth[1,2-e]-[1,3]oxazines being the products of aminoacetalization of aldehydes with 1-(α-aminobenzyl)-2-naphthol (Betti base).

Phosphorus (P) is a key biological element and a limiting nutrient in aquatic and terrestrial environments. The vast majority of organisms can only uptake phosphorus in its most oxidized form, as phosphate (PO43−), whereas a few prokaryotic species can metabolize phosphorus in a more reduced sta...

RésuméIntroductionLe méthotrexate à haute dose (supérieure ou égale à 1 g/m2) est utilisé pour traiter certaines hémopathies malignes et ostéosarcomes. La survenue d’une insuffisance rénale aiguë est une complication classique qui favorise le retard d’élimination du méthotrexate e...

BackgroundThere is no mainstay protocol for management of Methotrexate-induced oral ulcers; commonly used protocols are cessation of Methotrexate, folic acid treatment, corticosteroids or combination. A new era of oral ulcers management is represented by platelet concentrates. The current study ...

The design and construction of tumor-targeted drug delivery systems provide us a facile method to treat cancers selectively. In this work, a biotin-targeting inclusion complex based on β-cyclodextrin and methotrexate was designed and prepared successfully. Compared with other delivery systems, ...

Montmorillonite Clay (MMT) is aimed to develop as an orally administrable drug delivery vehicle with enhanced efficacy. Aiming to enhance the therapeutic index of methotrexate, curcumin is concomitantly used with methotrexate in the present study. Being folate antagonist in nature, methotrexate ...

Methotrexate, a chemotherapeutic agent, was found to undergo self-sensitized photodegradation in aqueous environments. As the initial concentration increased, methotrexate was able to enhance its own direct photolysis reaction not only in DI but also in natural waters. The methotrexate degradati...

Diphenylphosphide ion undergoes a photostimulated reaction with 7,7-dibromobicyclo[4.1.0]heptane and 1,1-dibromo-2,2,3,3-tetramethylcyclopropane involving both ionic and radical steps to afford cyclopropyldiphenylphosphines resulting from substitution and reduction.

Reactions of KPPh2 with the dimeric cyclopalladated complex {Pd(N∩C)(μ-Cl)}2 1 derived from N,N-dimethylbenzylamine were studied using different reagent ratios, temperature, reaction time, concentrations and solvents. Complex 3, the μ-Cl-μ-PPh2 analog of 1, was obtained in 76% yield in the o...

Reaction of [U(Cp*)2(Me)2] with HPPh2 was the best route to the uranium(IV) diphenylphosphide compounds [U(Cp*)2(PPh2)2] 1 and [U(Cp*)2(PPh2)(Me)] 2. Thermolysis of 2 afforded the ortho-metallated complex [U(Cp*)2(PPh{o-C6H4})] 3. Reduction of 1 with KH gave the first U(III) phosphide K[U(Cp*)2(...

We have measured anion photoelectron spectra of the diphenylphosphide anion, PPh2-, and its oxide, POPh2-, the former being a ligand which is often utilized in inorganic chemistry. From these spectra, we have determined the vertical detachment energies of these anions and estimates of the electr...

Protein oxidation is a major pathway for degradation of biologic drug products. Past literature reports have suggested that 2,2-azobis (2-amidinopropane) dihydrochloride (AAPH), a free radical generator that produces alkoxyl and alkyl peroxyl radicals, is a useful model reagent stress for assess...

In this study, the solubility of L-histidine in three different binary solvents, including (water + acetone), (water + ethanol) and (water + 2-propanol), was measured over temperatures from 283.15 K to 318.15 K by employing a gravimetric method at atmospheric pressure. It was found that the solu...

Oxidation can be induced by multiple processes in biological samples, with proteins being important targets due to their high abundance and reactivity. Oxidant reactions with proteins are not comprehensively understood, but it is known that structural and functional changes may be a cause, or a ...

Drugs interacting with the histaminergic system are currently used for vertigo treatment and it was shown in animal models that structural analogues of histamine like betahistine improved the recovery process after vestibular lesion. This study was aimed at determining the possible dose and dura...

SummaryCTP synthase (CTPS) forms compartmentalized filaments in response to substrate availability and environmental nutrient status. However, the physiological role of filaments and mechanisms for filament assembly are not well understood. Here, we provide evidence that CTPS forms filaments in ...

The Taylor dispersion technique has been used for measuring mutual diffusion coefficients of l-histidine methyl ester as its dihydrochloride at T = 298.15 K and finite concentrations from (0.001 to 0.100) mol · dm−3. On the basis of experimental mutual diffusion coefficients, the hydrodynamic r...

Nonlinear optical single crystal of α-L-Histidine dihydrochloride (α-LHDC) and β-L-Histidine dihydrochloride (β- LHDC) was synthesized by saturated solution was prepared at room temperature and it was kept at room temperature for the slow evaporation. The grown crystals were characterized us...

Amination of acetonitrile by the amines MeNH2, PrnNH2, PriNH2, ButNH2, and Et2NH is efficiently promoted by the lanthanide iodides LnI2 (Ln = Nd, Dy, Tm), LnI3 (Ln = Pr, Nd, Dy) and LnI3(THF)3 (Ln = Pr, Nd, Dy). The formed mono- and N,N′-disubstituted amidines MeC(NH)NHR (R = Pri, But), MeC(NH)...