A new separation method based on a novel reversed-phase sequential injection chromatography (SIC) technique was used for simultaneous determination of ambroxol hydrochloride and doxycycline in pharmaceutical preparations in this contribution.The coupling of short monolith with SIA system results...

Potential usage of biodegradable and biocompatible polymeric nanofibers is the most attention grabbing topic for the drug delivery system. In order to fabricate ultrafine fibers, electrospinning, one of the well-known techniques, has been extensively studied in the literature. In the present stu...

In this work, g-C3N4 thin layer @ CeO2 core-shell composite photocatalyst was synthesized by simple hydrothermal and in situ synthesis method for the first time, and it was combined with hydrogen peroxide (H2O2) to degrade the antibiotic doxycycline hydrochloride (DOX) under visible light. A ser...

Clay minerals have been used as adsorbents for decades but research into their use within healthcare as drug-carriers and modified drug release materials is an increasingly common area of interest. In current clinical practice the management of acute bacterial skin and skin-structure infections ...

Niosomes have been considered as promising nanoscale carriers for ocular drug delivery, since they have been shown to increase the bioavailability of various drugs and to improve their efficacy. The main objective of this study was to prepare and characterize niosomes for ocular delivery of doxy...

Kefiran is a microbial biopolymer which is able to form nanofibers with specific characteristic features. The present study demonstrated the successful fabrication of drug loaded kefiran nanofibrous mats by electrospinnig method. Nanofibrous mats were characterized using Scanning Electron Micros...

Natural-occurring polymer intercalated inorganic clay composites have received increasing interests in water cleanup for the features of eco-friendliness, cost-effectiveness, and availability. Herein, a new lignin xanthate resin (LXR) intercalated bentonite clay composite (LXR-BT) for the adsorp...

In this study, the effect factors and mechanisms of doxycycline hydrochloride (DOX) adsorption on copper nitrate modified biochar (Cu-BC) was investigated. Cu-BC absorbent was synthesized through calcination of peanut shells biomass at 450 °C and then impregnation with copper nitrate. The Cu-BC...

In order to effectively control the bacterial pneumonia in pigs, doxycycline hydrochloride (DoxHcl) and florfenicol (FF) microparticle suspension together with inclusion complexes was prepared by using hydroxypropyl-β-cyclodextrin (HP-β-CD) as host molecules, polyvinylpyrroliddone (PVP) as pol...

We previously reported that a conjugate of hyaluronic acid (HA) and methotrexate (MTX) could be a prototype for future osteoarthritis drugs having the efficacy of the two clinically validated agents but with a reduced risk of the systemic side effects of MTX by using HA as the drug delivery carr...

In this study, the affinity profile of idaverine for the M1- (neuronal tissue), M2- (heart) and M3- (glandular tissue/nonvascular smooth muscle) muscarinic receptors was examined by means of radioligand binding and in vitro organ bath experiments in order to use the compound for the investigatio...

d-Amino acid oxidase (DAAO) is a potential target in the treatment of schizophrenia as its inhibition increases brain d-serine level and thus contributes to NMDA receptor activation. Inhibitors of DAAO were sought testing [6+5] type heterocycles and identified isatin derivatives as micromolar DA...

Hot-water soluble polysaccharides H-1-3 and H-2-1 were isolated from the thalli of the lichen Xanthoria parietina (L.) Th. Fr. and purified by ion exchange and gel permeation chromatography. Structure elucidation was mainly based on 2D-NMR and nano-ESI-Q-TOF MS/MS experiments.H-1-3 (13.7 kDa) wa...

β-Mannosidase (EC 3.2.1.25) is an exoglycosidase specific for the hydrolysis of terminal β-1,4-glycosidic linkage in mannan which can be applied in the food manufacture, animal feed, bioethanol making and coffee extraction industries. A novel β-mannosidase gene (Lrman5A) from Lichtheimia ramo...

The glycoside hydrolase family 5 (GH5) endo-β-1,4-mannanases ManA and ManC from Aspergillus nidulans FGSC A4 were produced in Pichia pastoris X33 and purified in high yields of 120 and 145 mg/L, respectively, from the culture supernatants. Both enzymes showed increasing catalytic efficiency (kc...

This work aims to produce glutathione directly from mannan-based bioresources using engineered Saccharomyces cerevisiae. Mannan proved to be a valuable carbon source for glutathione production by this organism. Mannan-hydrolyzing S. cerevisiae was developed by heterologous expression of mannanas...

A GH 134 β-1,4-mannanase SsGH134 from Streptomyces sp. NRRL B-24484 possesses a carbohydrate binding module (CBM) 10 and a glycoside hydrolase 134 domain at the N- and C-terminal regions, respectively. Recombinant SsGH134 expressed in Escherichia coli. SsGH134 was maximally active within a pH r...

Endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans and offers many applications in different biotechnology industries. Purification and kinetic properties of the endo-1,4-β-mannan...

A mannan-degrading enzyme was isolated from the digestive fluid of the common sea hare Aplysia kurodai by ammonium sulfate fractionation followed by conventional column chromatography. The purified enzyme, named AkMan in the present paper, showed a single band with an approximate molecular mass ...

ABSTRACTThe objective of the current study was to compare nursery pigs fed endo-1,4-β-d-mannanase enzyme (Hemicell HT 1.5x) and 1% added fat with those fed a control diet (no enzyme) and increasing added fat. Soybean meal contains β-mannans, which are polysaccharides in cell walls of legumes. ...