Neonicotinoids are the most prominent group of insecticides in the world and are commercialized in over 120 countries for the control of agricultural pests mainly due to their broad-spectrum activity and versatility in application. Though non-target soil organisms are likely to be exposed during...

The detection and monitoring of acetamiprid has drawn extensive attentions, due to their potential threat to human health. Herein, a simple, sensitive and label-free fluorescent assay based on triplex-to-G-qadruplex (TTGQ) molecular switch, was developed for the assay of acetamiprid in aqueous s...

Humic substances (HS) in soil and sediments, and surface water influence the behavior of organic xenobiotics in the environment. However, our knowledge of the effects of specific HS fractions, i.e., humic acids (HAs), fulvic acids (FAs), and humin (HM), on the sorption of organic xenobiotics is ...

We previously reported that cytochrome P450 s play critical roles in neonicotinoid insecticide biodegradation by white-rot fungi. Here, we investigated the biodegradation of acetamiprid (ACET) by Phanerochaete chrysosporium to identify the cytochrome P450 involved in this degradation process. Du...

In present work, a versatile “signal-on” electrochemical aptasensor with ultra-sensitivity and high selectivity for detecting acetamiprid residues has been successfully constructed. Electrochemistry behaviors of as-synthesized copper-centered metal-organic frameworks (CuMOF) on various electro...

An upconversion fluorescence DNA probe which consists of aptamer-conjugated magnet nanoparticles (apt-MNPs) and complementary DNA-conjugated upconversion nanoparticles (cDNA-UCNPs) was developed to detect acetamiprid. Acetamiprid can specifically conjugate with the apt-MNPs to dissociate the cDN...

As a novel neonicotinoids insecticide, acetamiprid has been widely used worldwide. In this study, a laboratory test was conducted to expose earthworms (Eisenia fetida) to artificial soil spiked with various concentrations of acetamiprid (0, 0.05, 0.10, 0.25 and 0.50 mg/kg of soil) respectively a...

The Yb-doped PbO2 electrodes were fabricated by electrodeposition method. SEM and XRD experiments revealed that the adulteration of Yb can decrease the crystal grain size and make the electrodes more compact. Electrochemical measurements, accelerated service lifetime and bulk electrolysis experi...

A label-free and enzyme-free aptasensor for sensitive assay of acetamiprid has been established using AT-rich double-stranded (ds) DNA-templated copper nanoparticles (CuNPs) as fluorescent probe. In this work, two hairpin DNA, HP1 and HP2, were elaborately designed with AT-rich DNA sequences in ...

Farmland bird species are particularly exposed to pesticides through various pathways. Among pesticides, neonicotinoids insecticides are commonly used in agriculture, but their influence on bird reproductive capacities is poorly understood. In this study, we experimentally tested the effects of ...

Using human red blood cell ghost membranes, we have evaluated 5-nitro-2-[N-3-(4-azidophenyl)-propylamino]-benzoic acid and 5-nitro-2-[N-3-(4-azido-2,3,5,6-tetrafluorophenyl)-propylamino]-benzoic acid (FAzNPPB) as photoaffinity labeling agents based on the structure of the widely important Cl− ch...

A photoaffinity analog of the potent epithelial chloride channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoic acid has been synthesized and characterized. In the dark, this reagent, 5-nitro-2-[N-3-(4-azidophenyl)-propylamino]-benzoic acid, and the parent compound reversibly inhibited chloride...

Ten hemoglobins from the teleost Micropogon undulatus were separated in native polyacrylamide gel electrophoresis. In a large sample taken from a tributary of the Chesapeake Bay in Virginia, the distribution of these hemoglobins confirmed a polymorphic condition, which was previously known in No...

Asymmetrical hybrid hemoglobins formed from mixtures of oxyhemoglobins S and F and A and F were separated by high-performance liquid chromatography on a 4.6 × 250 mm wide-pore polyethyleneimine-silica gel column under anaerobic conditions. The resulting HPLC chromatogram showed three peaks, wit...

Ethylene oxide (EO), acrylamide (AA) and glycidamide (GA) exposures are associated with mammary tumors in animals. Currently available information about human exposure to these chemicals is limited creating the need for analytical methods to assess their exposure. We developed a sensitive ultra-...

To address complex thermodynamic systems with multiple interacting events, we have developed the concept of hierarchical thermodynamic interactions. In this study, this concept is extended to protein-ligand systems with similar but not identical protein subunits, and applied to the analysis of p...

This study examined the effect of submaximal and high-intensity (sprint) training on venous hemoglobin concentration ([Hb]) in Thoroughbred (Tb) racehorses before and after high-intensity exercise on the gallops. Measurements for n = 14 Tb yearlings took place during high-intensity exercise on a...

IntroductionThere are continuing needs for new antithrombotic agents and procedures. We hypothesized that the slowly cleared recombinant fusion proteins barbourin–albumin (BLAH6) and hirudin–albumin (HLAH6) would be effective in limiting fibrin(ogen) and/or platelet deposition in a rabbit mode...

The X-ray structure of the amino acid N-(tert-butoxycarbonyl)-l-alanine (B-l-ALA) has been determined and its IR spectrum interpreted. The molecules are held together by intermolecular OH···O hydrogen bonds between the carboxyl groups which are in a cyclic arrangement. Weaker hydrogen bonds a...

The tert-butoxycarbonyl protecting group for amines, alcohols, and thiols was removed efficiently (90–99% yields) by use of 0.20 equivalent of Ce(NH4)2(NO3)6 in acetonitrile.