One of recombinant hirudin variants, rHV2, a polypeptide used as an anticoagulant agent in clinic, was administered to anesthetized rats via intratracheal, buccal, nasal and rectal routes. Prolongation in clotting time and thrombin time was measured to calculate pharmacological bioavailability. ...

Osmotic stress, together with weakened antioxidant defense mechanisms, is attributed to the changes observed in human diabetic cataract. The use of hirudin, an antithrombic agent, in the pathogenesis of human cataracts has not been studied so far. Since the epithelium is the metabolic unit of th...

The possibility for oral administration of peptide recombinant hirudin variant (rHV2-K47) as an anticoagulant agent was evaluated in several aspects. The proteolytic properties of rHV2-K47 and its stability during storage were examined by in vitro experiments. Radiolabeled rHV2-K47 was infused i...

Oxidative processes in the lenses are the most commonly found damaging factor for the development of cataracts. Hirudin, a most potent inhibitor of thrombin as an antithrombic drug, also have potential use in cataracts. In order to investigate the mechanisms of hirudin against galactose-induced ...

A synthetic medium, TK-25, for high cell density cultivation (HCDC) of Escherichia coli K-12 was modified to support HCDC of strain JM109. By optimizing the culture conditions, the cell concentration of 65 g/l in 14 h was obtained in the optimized medium, namely TK-10, with glucose-fed batch cul...

In the present study, HPLC–ESI–IT (ion-trap) MS was used for carboxyterminal (C-terminal) amino acid sequence confirmation of intact recombinant Hirudin Variant 3 (HV3) and alkylated HV3. The C-terminal amino acid sequence of HV3 was determined by the use of carboxypeptidase P (CPP), and by th...

Recombinant fusion proteins consisting of hirudin variant 3 (HV3) fused at its C-terminus to either of two forms of mature rabbit antithrombin (AT) were generated in COS-1 cells. HV3 fused to wild-type AT was designated HAT, while a similar chimeric protein in which the P12 residue of AT was mut...

Thalassemia is an inherited blood disorder which is divided into two groups: alpha and beta. HBA1 and HBA2 are the two genes associated with alpha thalassemia. The aim of this study is to investigate abnormal hemoglobin variants of alpha globin gene in healthy abnormal hemoglobin carrying indivi...

RésuméQuatre variants de l'hémoglobine humaine affectant la position α126 (H9), normalement occupée par un aspartate, ont été rapportés dans la littérature. Il s'agit des Hbs Montefiore (→ Tyr), Tarrant (→ Asn), Fukutomi (→ Val) et Sassari (→ His). Dans cette étude, nous ...

IntroductionOptimising haemoglobin (Hb) levels less than 13 g/dl in the preoperative period can reduce the transfusion rate. With this aim, we developed a multidisciplinary protocol in our hospital for the treatment of patients proposed for colorectal cancer surgery.

The effluents from sewage treatment plants strongly influence the water quality and flow of Mediterranean streams. These effluents play a crucial role in maintaining the aquatic communities of these ecosystems, particularly in the absence of natural flow resulting from climate constraints or int...

ObjectiveTo assess the pattern of glycosylated hemoglobin (HbA1c) requests by clinicians from eight health care departments by calculating indicators of demand appropriateness.

The aim of this cross-sectional study was to establish the prevalence of renal involvement and to identify associations with its most important possible risk factors in a group of patients with Type II diabetes mellitus, representative of the population living in Catalonia. One thousand two hund...

In this paper, a novel optical probing strategy for the detection of amantadine has been successfully developed based on the competitive host–guest interaction of amantadine and rhodamine6G (R6G) with mono-[6-(2-aminoethylamino)-6-deoxy]-β-cyclodextin (EDA-CD). We synthesized the EDA-CD and gr...

Three new copper (II) complexes, [Cu(L)(H2O)]ClO4 (1), [Cu(L1)(ClO4)]+ (2) and [Cu(L1)]2+ (3), where HL = 2-(E)-(2-(2-aminoethylamino)methyl)-4-bromophenol, L1 =(19E, 27E)-N1,N2-bis(phenyl(pyridine-2-yl)-methylene)-ethane-1, 2-diamine, have been synthesized and characterized by using various phy...

SU-88 [2′-carboxymethoxy-4,4′-bis(3-methyl-2-butenyloxy)chalcone] inhibited the activity of the prostaglandin (PG)-metabolizing enzyme, 15-hydroxy-PG-dehydrogenase (15-OH-PG-DH), in a cytoplasmic fraction of gastric mucosa. This compound had no effect on the PG synthetase of bovine seminal ves...

An antiulcer agent, 2′-carboxymethoxy-4,4′-bis(3-methyl-2-butenyloxy) chalcone (CBC), formed inclusion complexes with α-, β- and γ-cyclodextrins (α-, β- and γ-CyDs) in molar ratios (guest:host) of 1:1 and 1:2 in water. The low aqueous solubility of CBC (1.1 × 10−5 M) was significantly i...

The inclusion complexation of 4-t-butyl-2′-car☐ymethoxy-4′-(3-methyl-2-butenyloxy)chalcone (SU-740), a newly developed antiulcer agent, with β-cyclodextrin (β-CyD) in water and in solid state was investigated for the purpose of improving the low aqueous solubility and oral bioavailability. S...

Fluorescence quantum yields and time-correlated lifetimes for the exciplex-forming molecule 6-(2',3'-butenyloxy)methyl-1-cyanonaphthalene (1) have been obtained in ethyl acetate in the temperature range 0–40°C. Molecular mechanics and extended Hückel calculations have been used to p...

Salinity is one of the most important abiotic stresses causing a significant reduction of crop plants yield. To gain a better understanding of salinity tolerance mechanisms in barley (Hordeum vulgare), we investigated the changes in root proteome of salt-sensitive (DH14) and tolerant (DH187) lin...