A direct method for the quantitative determination in grapes of four known precursors of the varietal aromas of the thiol character of wine has been optimized and validated. A small volume of centrifuged and filtered must is directly injected in the ultrahigh liquid performance chromatography co...

The grassy characteristic aroma perceived in brewed sake made from low-glutelin rice (Oryza sativa L. Mizuhonoka) was examined by gas chromatography–olfactometry and gas chromatography–mass spectrometry. By comparing the odor properties and Kovats retention indices to those of standard compoun...

Crystal and molecular structures of two silatranes, 1-iodomethyl-3-methyl- and 1-iodomethyl-3,7,10-trimethyl-2,8,9-trioxa-5-aza-1-silabicyclo[3.3.3]undecanes, were determined by X-ray analysis. Comparison of their structural and geometrical characteristics with those of other silatranes shows th...

Crystals of N-(3,5-diXsalicylidene)-2-methoxyaniline (X = fluoro, chloro, bromo, and iodo) were prepared, and their structural and luminescence properties in the solid state were investigated. Only fluorescent orange crystals were obtained for N-(3,5-difluorosalicylidene)-2-methoxyaniline (1). B...

ABSTRACTThe use of point-of-care (POC) devices to measure blood metabolites, such as β-hydroxybutyrate (BHB), on farm have become an important diagnostic and screening tool in the modern dairy industry. The POC devices allow for immediate decision making and are often more economical than the u...

In view of the world's immense dependency on non-renewable fossil fuels, the present study explores the prospect of Anabaena variabilis and Microcystis aeruginosa, which represent two distinct groups of cyanobacteria: nitrogen fixers and non fixers, for bioethanol production by analyzing th...

We have previously reported in vivo biosynthesis of 2-hydroxyacid containing polyesters including polylactic acid (PLA), poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)], and poly(3-hydroxybutyrate-co-2-hydroxybutyrate-co-lactate) [P(3HB-co-2HB-co-LA)] employing metabolically engineered Escheri...

Poly (3-hydroxybutyrate) (P(3HB)), a type of bioplastic, has been attractive as an alternative source to replace petroleum-based plastics. Generally, P(3HB) is produced from Cupriavidus necator DSM 545 under some nutrient-limiting and oxygen-limiting conditions. In this work, the batch productio...

ABSTRACTThe objective of this study was to determine the effect of varying levels of sodium butyrate (SB) supplementation in feed on the growth, digestibility, and health of postweaned heifers. Forty Holstein dairy heifers with a mean age of 84 d and average body weight (BW) of 100.9 ± 11.2 kg ...

Poly[(R)-lactate-co-(R)-2-hydroxybutyrate], which is biosynthesized as a random co-polyester by microbial organisms, with various ratios of lactic acid (LA) to hydroxybutyrate monomers has been isothermally crystallized to investigate its thermal properties and crystal structure changes. Differe...

In our previous study, artificial polyhydroxyalkanoate (PHA) poly[(R)-2-hydroxybutyrate] [P(2HB)] was successfully biosynthesized from racemic 2HB in recombinant Escherichia coli using an engineered PHA synthase, PhaC1Ps(S325T/Q481K). Although P(2HB) has promising material properties, the low le...

Phenylacetic acid derivatives, methyl 2-acetyl-3,5-dihydroxyphenylacetate (1) and methyl 2-acetyl-5-hydroxy-3-methoxyphenylacetate (3); curvulin or ethyl 2-acetyl-3,5-dihydroxyphenylacetate (4), a known metabolite of Curvularia siddiqui, and 4-epiradicinol (5) have been isolated from the culture...

A total of 11 novel phosphoramidites, 3a–d, 4a–d and 14a–c were prepared from 2′-deoxyuridine functionalized at 5 and 6-position of the pyrimidine ring with hapten reporter groups, e.g. adamantane, carbazole, dansyl and dabsyl, suitable for use in immunodetection nucleic acid testing assays.

The effect of the simultaneous ingestion of soymilk and coffee on the urinary excretion of isoflavones (ISO) and chlorogenic acids (CGA) was investigated for the first time in humans. In a randomized crossover study, soy (79.7 µmol ISO), coffee (561.2 µmol CGA) and soy–coffee beverage (79.7 µ...

Rhodospirillum rubrum reaction centre (RC) complexes with or without antenna bacteriochlorophyll have been incorporated into liposomes; the latter were associated with a phospholipid-impregnated collodion film, and flash-induced electric responses were measured. It is shown that in the antenna-f...

The steady-state kinetics of the NADH dehydrogenase activity of the three-subunit flavo-iron-sulfur protein (FP, Type II NADH dehydrogenase) in the presence of the one-electron acceptor hexammineruthenium(III) (HAR) were studied. The maximal catalytic activities of FP with HAR as electron accept...

The steady-state kinetics of the NADH dehydrogenase activities of the mitochondrial NADH-ubiquinone oxidoreductase in the presence of one-electron acceptors, ferricyanide and hexammineruthenium(III), were studied. Similar to ferricyanide, hexammineruthenium was found to be an efficient electron ...

Claisen–Schmidt condensation of 3-formyl-9-methylcarbazole with various amides of 3-aminoacetophenone afforded N-{3-[3-(9-methyl-9H-carbazol-3-yl)-acryloyl]-phenyl}-benzamide/amide derivatives. All compounds were investigated for their in vitro xanthine oxidase (XO), tyrosinase and melanin prod...

Evidence is presented demonstrating the ability of Ralstonia eutropha A5 to degrade 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD) aerobically. Strain A5 was able to effect significant transformation of [14C]DDD: the hexane extractable radioactivity decreased to approximately 50% of the contro...

A 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degrading bacterium strain IITR03 producing trehalolipid was isolated and characterized from a pesticides contaminated soil. The strain IITR03 was identified as a member of the genus Rhodococcus based on polyphasic studies. Under aqueous cult...