Chymosin (Rennin) was effectively purified using an AH-Sepharose 4B column. Binding of Streptomyces pepsin inhibitor (acetyl-pepstatin) with chymosin was studied spectroscopically. The binding caused ultraviolet difference and CD spectral changes suggesting microenvironmental changes around tryp...

The present article reports a low molecular weight aspartic protease inhibitor from a Streptomyces sp. MBR04 exhibiting a two-step inhibition mechanism against pepsin. The kinetic interactions revealed a reversible, competitive, slow-tight binding inhibition with an IC50 and Ki values of 4.5 nM ...

Bongkrekic acid (BKA) is a strong inhibitor of adenine nucleotide translocase (ANT), inducing inhibition of adenosine triphosphate synthesis. We designed and synthesized simplified benzene-ring-containing BKA analogs. The key reaction is the one-pot double Sonogashira reaction, which forms the m...

ObjectiveIncreasing the amounts of functionally competent brown adipose tissue (BAT) in adult humans has the potential to restore dysfunctional metabolism and counteract obesity. In this study, we aimed to characterize the human perirenal fat depot, and we hypothesized that there would be region...

Volatile compounds, including γ-lactones, in brown and white rice fermented by Lactobacillus paracasei, were compared using gas chromatography-mass spectrometry (GC–MS) with stir-bar sorptive extraction (SBSE). The contents of most esters, alcohols, lactones and some aldehydes were higher in b...

The performance of calcium nanoparticles (CaNPs) blending in ascorbic acid (AA) to alleviate internal browning symptoms of ‘Hindi Be-Sennara’ mango fruit under cold storage otherwise the impacts of CaNPs-AA on browning enzyme activities such as polyphenol oxidase (PPO, EC: 1.14.18.1) and Pheny...

The role of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in browning and thermogenesis has not been fully elucidated. Thus, we meant to evaluate the effect of EPA and DHA, administered alone or combined, with the activation of browning markers in subcutaneous white adipose tissue (...

The pericarp browning is an important physiological index during the postharvest storage, which seriously shortens the shelf-life of litchi fruit. In this study, the browning index of four litchi cultivars were compared, and the shelf-life, from longer to shorter, was ‘Feizixiao (FXZ)’, ‘Jing...

The role of Reactive Carbonyl Species (RCS) derived from the Maillard reaction and ascorbic acid degradation on brown color formation was investigated in orange juice during storage. Eight RCS were monitored in aseptic juice over an 8-week period under refrigerated (4 °C) and accelerated condit...

Long-term spaceflight has been proven to induce metabolic dysfunction. Brown adipose tissue (BAT) plays an important role in whole-body energy metabolism. We speculated that BAT function would change under microgravity. Here, we employed a tail suspension (TS) rat model to simulate the effects o...

Relationship among pH, generation of free amino acids (FAAs), and Maillard browning was investigated in Japanese common squid during air drying at 40 °C to prevent its discoloration. In the surface color measurement, increase in the b* value which is an indicator for Maillard browning of the dr...

The electrical and dielectrical properties of C.I. Basic Violet 10 were investigated using both DC and AC measurements. The DC electrical conductivity of the dye exhibited similar behavior to that of organic semiconductors and the mechanism of the AC conductivity the dye can be attributed to cor...

The formation of the inclusion complexes of Basic Violet 2 (New Fuchsin) with hydroxypropyl-β-cyclodextrin (HP-β-CD) was studied by UV–Vis absorption and fluorescence spectroscopy. The binding constants of the inclusion complexes, KB, were obtained considering the maximum shift of the absorpt...

The pseudoternary phase diagrams of surfynol465/n-butanol/n-hexane/0.006 mol/L NaOH aqueous solution with km = 5/3 and surfynol465/n-butanol/n-hexane/0.006 mol/L NaOH-heavy water solution with km = 5/3 (where km is mass ratio of surfynol465 to n-butanol) have been constructed. In the correspondi...

A great deal of the earlier research regarding ultraviolet light was conducted in laboratory environments, rendering it difficult to interpret fluorescence at the scene of a fire. The purpose of this research project is to explore the ways in which ultraviolet light can be used as a tool in fire...

The 129-residue lysozyme has been shown to form amyloid fibrils in vitro. While methylene blue (MB), a compound in the phenothiazinium family, has been shown to dissemble tau fibril formation, its anti-fibrillogenic effect has not been thoroughly characterized in other proteins/peptides. This st...

The concept of blue growth is the newest of many recent calls for more holistic management of complex marine social-ecological systems. The complexity of ocean systems, combined with limitations on data and capacity, demands an approach to management that is pragmatic—meaning goal- and solution...

The usefulness of Toluidine blue dye in the detection of anogenital injuries in sixty females aged 16–40 years of age with a history of consensual sexual intercourse within 48 h of intercourse were included for the study from the Gynaecology outpatient department of the Institute of Medicine, M...

The recent years have witnessed a rise in interest in the ocean economy. To cover a more sustainable dimension, terms such as ‘blue economy’ and ‘blue growth’ have been coined, and are increasingly used in international contexts and academic literature. However, there are no generally accept...

The determination of the chemical characterization and the order of ink crossing by ballpoint pens have received increasing and important attention in forensic analysis due to forgery or alteration of documents strongly related to their use. The examination of these cases must be using non-destr...