The formation of reactive oxygen species (ROS) could cause cellular damage and eventually lead to apoptosis and necrosis. The ratio between oxidized glutathione and reduced glutathione (GSSG-to-GSH ratio) has been used as an important in vitro and in vivo biomarker of the redox balance in the ce...
BackgroundThe term GSSG/GSH redox potential is frequently used to explain redox regulation and other biological processes.
The continued expansion of the fields of photoelectrochemical (PEC) aptasensor has guided the development of photoactive materials that can generate electron-photo pairs to emerge photocurrent. However, only such an improvement has an insatiable appetite for the application of molecular level fo...
Glutaredoxin (Grx) is a major redox enzyme that reduces disulfide bonds using glutathione (GSH) as an electron donor. The anaerobic bacterium Clostridium oremlandii possesses a selenocysteine-containing Grx (cGrx1) and a cysteine-containing homolog (cGrx2). Here, the crystal structure of the GSS...
Variability in the levels of GSH and GSSG in plasma is suggested to derive from inadequate pre-processing methods. The aim of this study was to develop a protocol for comparable and reliable measurements of GSH/GSSG. Venous blood from 8 healthy individuals were collected and divided into 7 diffe...
Early detection of infectious bacteria is a necessity for combating infectious diseases. Due to low infectious dose of Shigella, rapid and sensitive detection is needed. Compared to the presented genes, Spa gene can be introduced as a novel sequence for all species of Shigella detection. Herein,...
Carbon dots (CDs) are a type of metal-free fluorescent quantum dots and can be synthesized facilely and cost-effectively. In this work, we develop a one-pot solvothermal method to prepare a novel type of quaternized CDs using two commercial reagents (glycerol and dimethyloctadecyl[3-(trimethoxys...
The strain, Burkholderia vietnamiensis C09V was immobilized on PVA–alginate–kaolin gel beads as a biomaterial to improve the degradation of crystal violet from aqueous solution. The results show that 98.6% (30 mg L−1) crystal violet was removed from aqueous solution using immobilized cells on ...
A series of highly functional methacrylated bio-based resins were synthesized and used in the formulation of UV curable coatings systems. Methacrylated epoxidized sucrose soyate (MESS) and dimethacrylated epoxidized sucrose soyate (DMESS) were synthesized from epoxidized sucrose soyate (ESS). Th...
Redox-sensitive fluorescent proteins (roFPs) are a powerful tool for imaging intracellular redox changes. The structure of these proteins contains a pair of cysteines capable of forming a disulfide upon oxidation that affects the protein conformation and spectral characteristics. To date, a pale...
Infrared thermography is a noninvasive imaging technique used to determine surface temperatures under controlled conditions. This technique has many applications in different fields, the assessment of sport equipment being one of them. This chapter aims to provide a general overview of the scien...
Multilayer ultrathin films of poly(3,4-ethylenedioxythiophene) (PEDOT)/stearic acid (SA) were fabricated using a modified Langmuir–Blodgett (LB) technique. The formation of LB ultrathin film was confirmed by UV–vis–NIR absorption spectrum and X-ray photoelectron spectroscopy (XPS) technique. ...
Turmeric (Curcuma longa L.) belongs to the family Zingiberaceae that is widely used as a spice in food preparations in addition to its biological activities. UV, FT-IR, 1H NMR in addition to HPLC were applied to construct a metabolic fingerprint for Turmeric in an attempt to assess its quality. ...
This chapter discusses the historical and technological development of two important techniques for fabricating ultrathin films: Langmuir–Blodgett (LB) and layer-by-layer (LbL) methods. For the LB method, the theoretical aspects of characterizing Langmuir monolayers by pressure and surface pote...
A method was developed for the confirmatory and quantitative analysis of one pyrethrin and 18 pyrethroid residues in animal fat. Fat was extracted was collected from adipose tissue melted in an oven at 65 °C for 2 h. Fat samples (1 g) were dispersed with deactivated Florisil® sorbent and extrac...
This paper reports comparative extraction efficiencies and enrichment methods for natural insecticidal compounds pyrethrins from the dried flowers of Chrysanthemum cinerariaefolium using different techniques and solvent systems. Four different extraction techniques viz. percolation, agitation wi...
The occurrence of horizontal transfer of the insect growth regulator (IGR) methoprene on confined populations of Tribolium castaneum (Herbst), either with or without hidden refugia, was determined through a series of experiments. Multiple applications were made with the IGR alone or combined wit...
Natural pyrethrins have been widely used for pest control in organic farming and for residential indoor pest managements. Although the specific mechanisms underlying their activity are incompletely understood, natural pesticides are considered the safest based on their target specificity and rap...
The extraction of pyrethrins from chrysanthemum flowers has been performed using supercritical CO2 (SC-CO2) extraction coupled to fractional separation, with the aim of producing complete and selective extraction of these insecticidal principles. 90 bar, 40 °C were selected as the operative con...
Pyrethrum is a perennial crop commercially grown for extraction of natural insecticidal compounds (pyrethrins), which accumulate in the achenes of the flower heads. Pyrethrum achenes are densely covered by glandular trichomes and these play a vital role in biosynthesis of pyrethrins. Pyrethrum i...
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