(−)-Cubebin is a lignan extracted from the seeds of the pepper Piper cubeba, a commonly eaten spice with beneficial properties, including trypanocidal, anti-inflammatory, analgesic, anti-proliferative and leishmanicidal activities. Because of its therapeutic potential, we investigated the effect...

Even though the Chagas’ disease, caused by the protozoan Trypanosoma cruzi, was described 100 years ago by Carlos Chagas, it still represents a major public health concern and is found in 18 developing countries in South and Central America. In Brazil, Benznidazole (Rochagan) is the only drug w...

(−)-Cubebin (CUB), isolated from seeds of Piper cubeba, was used as starting material to obtain the derivatives (−)-hinokinin (HK) and (−)-O-benzyl cubebin (OBZ). Using paw edema as the experimental model and different chemical mediators (prostaglandin and dextran), it was observed that both der...

(−)-Cubebin, isolated from the seeds of Piper cubeba, and its five different types of derivatives (a total of 17), with varying functionalities, were tested for their in vitro anticancer activity against six human cancer cell lines (A549, K562, SiHa, KB, HCT116 and HT29) using MTT assay. Cubebin...

A new method is presented for the determination of 2′-deoxythymidine 5′-triphosphate and 2′-deoxycytidine 5′-triphosphate concentrations within human cells based on a DNA polymerase reaction directed by a palindromic oligonucleotide precursor. Two 19-mer oligonucleotide precursors are employ...

We have previously reported that several β-l-thymidine analogues including β-l-3′-azido-3′-deoxythymidine (β-l-AZT), β-l-3′-fluoro-2′,3′-dideoxythymidine (β-l-FLT) and β-l-2′,3′-didehydro-2',3'-dideoxythymidine (β-l-D4T) did not inhibit HIV replication in human peripheral...

The rate of incorporation of labeled precursor into DNA of isolated nuclei decreased from gastrulation to the tailbud stage in developing frog embryos. The addition of saturating amounts of calf thymus DNA revealed that the activities of the enzyme(s) which incorporates deoxythymidine triphospha...

The enzyme reaction mechanism and kinetics for biosyntheses of deoxycytidine triphosphate (dCTP) and deoxythymidine triphosphate (dTTP) from the corresponding deoxycytidine diphosphate (dCDP) and deoxythymidine diphosphate (dTDP) catalyzed by pyruvate kinase were studied. The kinetic model for t...

Phospholipid conjugates of 3′-azido-3′-deoxythymidine (AZT) show activity against the human immunodeficiency virus (HIV) in vitro. In a previous report (K.Y. Hostetler, L.M. Stuhmiller, B.H.M. Lenting, H. van den Bosch and D.D. Richman (1991), J. Biol. Chem. 265, 6112–6117) the syntheses and ...

A comparison was made of the inhibition of deoxythymidine kinase derived froin Ehrlich ascites carcinoma cells by 5-iodo-2'-deoxyuridine 5'-triphosphate and deoxythymidine 5'-triphosphate. The inhibition produced by the halogenated triphosphate derivative was observed to be qualit...

In the present study, templates containing a specific segment of the Gγ-globin gene were constructed and incorporation of 3′-azido-3′-deoxythymidine 5′-triphosphate (AZT-TP) or 2′,3′-dideoxythymidine 5′-triphosphate (ddTTP) into these templates was compared to that observed in M13 bacteri...

3′-Azido-3′-deoxythymidine triphosphate (erythro) and its threo isomer were synthesized and investigated for their inhibition of HIV reverse transcriptase from virus isolate U 937/HTLV-III. The erythro isomer was a competitive inhibitor of the reaction directed by (rA)n(dT)12–18 and the Ki va...

Mitochondrial bioenergetics, mitochondrial reactive oxygen species (ROS) and cellular levels of nucleotides have been hypothesized as early indicators of Alzheimer's disease (AD). Utilizing relative decline of cognitive ability as a predictor of AD risk, we evaluated the correlation between...

The aim of this study was to standardize a method of DNA extraction from formalin-fixed and paraffin-embedded tissues (PETs) using a salt solution to precipitate protein and isopropanol to precipitate DNA. The samples were submitted to a DNA extraction method in which two different concentration...

Abiotic stresses reduce plant productivity. We focus on gene expression analysis following exposure of plants to high salinity, using salt-shock experiments to mimic stresses that affect hydration and ion homeostasis. The approach includes parallel molecular and genetic experimentation. Comparat...

Carbohydrate binding modules (CBMs) are non-catalytic domains within larger multidomain polypeptides. The CelH from Ruminoclostridium (Clostridium) thermocellum contains a family 11 CBM (RtCBM11) with high binding affinity for the linear polysaccharide β-glucan, and low affinity for the branche...

The hybrid complex of inorganic-organic [DAMS]2[CdI4] [[DAMS =4‐N, N‐dimethylamino‐N‐methyl‐stilbazolium iodide] was synthesized by metathesization of the 4-N,N-dimethylamino -N-methyl stilbazolium iodide [DAMS.I-] salt with cadmium acetate. The [DAMS]2[CdI4] single crystals were grown by s...

A convenient, diversity-oriented approach for the transformation of readily available amidoximes, o-hydroxyarylamines, acyl hydrazines, carboximidohydrazides and thiohydrazides into their respective N,N-dimethylamino-substituted azoles is described. The method is particularly suitable for array ...

The depigmenting effects of oxyresveratrol and trans-dihydromorin were investigated in b16F10 cell line and its synergetic melan-a cells, as well as in 3-dimensional skin models. But their inhibitory effects were different in two cell lines. In b16 cells, oxyresveratrol showed stronger depigment...

The synthesis of 4-amino-4-deoxy carbohydrates that occur in antibiotics and in bacterial glycans is described. The focus of the coverage is on literature published since 2000; however, earlier work is included in some cases for the purposes of historical context and to highlight methods develop...