Some new 1,3,4-triazolo-, 1,3,4-oxadiazolo-, 1,3,4-thiadiazol-, and pyrazolo-3,4-dimethylphenyl-1(2H)-oxo-phthalazine derivatives were synthesized and identified by IR, 1H NMR, MS and elemental analysis. Most of the newly synthesized products were tested for their anti-inflammatory activities. A...

Twenty-six compounds derived from 3′-aminocombretastatin A-4 (AmCA-4) containing a urea fragment mimicking the structure of Sorafenib, have been synthesized and evaluated as antiangiogenic compounds. Antiproliferative activity of all the synthetic ureas has been measured on tumor cell lines HT-...

The main objective of the present study was to characterize the inhibition by phenylalanine and phenylpyruvate of ATP diphosphohydrolase activity in synaptosomes from the brain cortex of rats. This enzyme participates together with a 5′-nucleotidase in adenosine formation from the neurotransmit...

Enzymatic cycling assays for the determination of l-phenylalanine and phenylpyruvate in deproteinized tissue extracts are described. Assay 1 couples glutamine transaminase K with l-phenylalanine dehydrogenase. Assay 2 combines phenylalanine dehydrogenase, l-amino acid oxidase, and catalase. In b...

Cells obtained from growth of Achromobacter eurydice, Pseudomonas aromatica and Pseudomonas putida on l-phenylalanine containing medium catalyzed the enzymatic acyloin condensation of phenylpyruvic acid 1 and acetaldehyde 2 by phenylpyruvate decarboxylase to produce 3-hydroxy-1-phenyl-2-butanone...

We discovered the phenyllactate (PLA)-producing fungal strain Wickerhamia fluorescens TK1 and purified phenylpyruvate reductase (PPR) from fungal cell-free extracts. The PPR used both NADPH and NADH as cofactors with more preference for the former. The enzyme reaction as well as the fungal cultu...

An NADH-dependent phenylpyruvate reductase (LaPPR) was identified through screening the shotgun library of Lactobacillus sp. CGMCC 9967. It belongs to d-3-phosphoglycerate dehydrogenase (PGDH) subfamily of 2-hydroxy acid dehydrogenase superfamily. LaPPR was stable at pH 6.5 and 30 °C, with a ha...

2-Hydroxy acid dehydrogenases (2-HADHs) have been implicated in the synthesis of 2-hydroxy acids from 2-oxo acids that are used in wide areas of industry. d-lactate dehydrogenases (d-LDHs), a subfamily of 2-HADH, have been utilized to this purpose, yet they exhibited relatively low catalytic act...

Eleven lanthanide compounds [Y(cinn)3] (1), [La(cinn)3] (2), [La(4-OMecinn)3]·2H2O (3), [La(4-Clcinn)3]·2H2O (4), [La(4-OMephac)3]·4H2O (5), [La(4-Clphac)3]·3H2O (6), [Ce(cinn)3] (7), [Nd(cinn)3] (8), [Sm(cinn)3]·H2O (9), [Yb(cinn)3] (10) and [Sm(4-OMephac)3]·H2O (11) containing carboxylat...

In this paper, the effectiveness of anionic p-phenylacetate film was evaluated in protecting physically small conical-tip carbon electrodes (∼2 μm radius and ∼4 μm axial length) from fouling during dopamine detection in vivo. After characterising p-phenylacetate film-modified carbon electrod...

Mixed trifluoroacetyl phenylacetyl anhydride and 3-halostyrenes (fluoro, chloro, and bromo) or vinylcycloalkanes (cyclohexyl and cyclooctyl), undergo cascade Friedel–Crafts cycli-acylalkylation, enolization, and O-acylation to give 4-substituted tetralen-2-ol phenylacetates, without additional ...

The analysis of the infrared carbonyl bands of some 2-ethylsulfinyl-(4′-substituted)-phenylacetates bearing the substituents NO2(1), Cl(2), Br(3), H(4), Me(5) and OMe(6), along with B3LYP/6-31G(d,p), SM5.42R solvation model calculations and natural bond orbital (NBO) analysis was carried out. T...

The authors evaluated the cell growth inhibition, reduction of tumourigenicity, and differentiation-inducing effects of sodium phenylacetate (NaPA) on a canine mammary tumour cell line. Treatment of the canine mammary tumour cell line (MCM-B2) with NaPA lead to the arrest of cell growth. Sodium ...

Sodium phenylacetate (NaPA) at concentrations ranging from 2 to 6 mM stimulated morphological differentiation of two human neuroblastoma cell lines IMR-32 and UKF-NB-3. These concentrations inhibited growth and DNA synthesis of the cells in a dose dependent manner without significant effect on c...

ABSTRACT: During 15 days of treatment of K562 cells with sodium phenylacetate, we observed an increase in the cellular hemoglobin concentration with a similar increase in the expression of γ-globin mRNA. Morphological studies demonstrated characteristic features of erythroid differentiation and...

The influence of lithium, sodium, potassium, rubidium and cesium cations on the electronic system of phenylacetic acid was studied. The FT-IR, FT-Raman and 1H and 13C NMR spectra were recorded for studied compounds. Characteristic shifts in IR and NMR spectra along alkali metal phenylacetates we...

Phenylacetate (PA) inhibits the growth of tumor cells in vitro and in vivo and shows promise as a relatively nontoxic agent for cancer treatment. A recent report shows that prolonged exposure of cells to low concentrations of PA can enhance the radiation response of brain tumor cells in vitro, o...

Background: Ammunol® (sodium phenylacetate/sodium benzoate) is an intravenously administered, investigational drug used for the treatment of acute hyperammonemia in infants, children, and adults with urea cycle enzyme deficiencies. A pharmacokinetic study of sodium phenylacetate/sodium benzoate ...

Molecular and tissue damage induced by reactive oxygen species is a serious consequence of the production of free radicals in biological systems. Biological markers produced by reactions with hydroxyl radicals are useful indices of free radical processesin vivo.In this respect, hydroxylation of ...

Taylor dispersion is used to measure the binary mutual diffusion coefficient (D) of aqueous solutions of sodium salicylate (NaSal), a non-steroidal anti-inflammatory drug (NSAID). The D° value estimated by extrapolation to zero NaSal concentration is used to calculate the limiting mobility of t...