We are reporting an innovative building-block for the development of biosensors based on the non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs) with avidin (MWCNTs-avidin). In this work, at variance with previous reports, avidin has the double role of simultaneously being t...
Human serum contains natural antibodies against avidin. Affinity purified natural anti-avidin human IgG exhibits affinity constants comparable to those of antibodies produced by active immunization of rabbits. Using a random hexapeptide library displayed on the filamentous M13 phage, and rabbit ...
Since the importance of the high affinity between avidin and biotin, Kd = 3 × 10−16 M, gained universal recognition, numerous chemical, biological and medical avidin-biotin based applications have been developed. However, in some cases the high affinity may be a disadvantage, as this interactio...
Biotinylated antibodies/antigens are currently used in many immunoassay formats in clinical settings for diversified analytes and biomarkers to offer high detection selectivity and sensitivity. Biotin cannot be synthesized by mammals and must be taken as an essential supplement. Normal intake of...
An interlaboratory study was performed on two high-performance liquid chromatographic methods to determine tylosin. The first method is a reversed-phase HPLC on a C18 column, while the second is a method using a polymeric stationary phase. The first method is described in several pharmacopoeia m...
Five studies were conducted to evaluate the ability of growth-promoting implants containing a pellet of tylosin tartrate [Component®(Ivy Animal Health, Overland Park, KS) with Tylan® (Elanco Animal Health, Indianapolis, IN)] to prevent implant site abscesses in feedlot cattle. Component® with Ty...
The antibacterial effects of tylosin tartrate, added to a cattle growth implant, were evaluated in terms of prevention of implant site abscess formation. An implant site abscess induction model was developed to create implant site abscesses reliably in test animals. This model was then used to t...
The solubility data of tylosin tartrate in methanol + (tetrahydrofuran or acetone) mixtures was determined at atmospheric pressure by a gravimetric method over temperature range of 293.15–323.15 K. The experimental results indicated that solubility of tylosin tartrate increases with increasing ...
Data on (solid + liquid) equilibrium of tylosin tartrate in {methanol + (ethanol, 1-propanol or 2-propanol)} solvents will provide essential support for industrial design and further theoretical studies. In this study, the solubility of tylosin tartrate in alcohol mixtures was measured over temp...
Extracellular DNA (eDNA), which is commonly detected in aquatic and terrestrial environments, may be involved in gene transfer, increases in genetic diversity, and evolution. However, it has been reported that some small organic molecules or heavy metal ions can influence the transformation of D...
A specific polyclonal antibody was prepared based on a new hapten with stable structure. Based on this, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established for determination of tylosin residues in food and environmental samples. The experimental conditions were op...
The purpose of this study was to determine the lethal and sublethal effects of the antimicrobial tylosin on natural estuarine phytoplankton communities. Bioassays were used in experimental treatments with final concentrations of 5 to 1000 μg tylosin l−1. Maximum percent inhibition ranged from 5...
ABSTRACTThe objectives of this study were to evaluate the effects of generic ractopamine (Actogain) with or without the combination of monensin and tylosin on growth performance, carcass characteristics, and tenderness of finishing steers. A total of 1,230 steers were finished in 6 blocks based ...
In a second attempt to design a simple gentamycin delivery system usable for local antibiotic therapy administered per-operatively, bioresorbable particles were made by grinding and sieving the solid masses resulting from the mixing of various amounts of gentamycin sulfate (GS) with two high mol...
ObjectiveTo investigate the ameliorative effect of dietary inclusion of garlic (Allium sativum) on gentamycin-induced hepatotoxicity in rats.
An enzyme-linked immunosorbent assay (ELISA) was developed using a polyclonal antibody with high affinity and specificity for detecting gentamycin in animal-derived foods. The half-maximum inhibition concentration (IC50) and limit of detection (LOD, calculated as IC15) of the ELISA for gentamyci...
Biodegradable films were prepared by casting acetone solutions of high and low molecular weight poly (dl-lactic acids), respectively PLA50P and PLA50p, on glass plates before drying. Under these conditions, PLA50p played the role of plasticizer for PLA50P, as shown from DSC and dynamic mechanica...
The current study investigated the renoprotective effects of stevia, angiotensin-II type 1 receptor (AT1) blocker and calcium (Ca2+) channel blocker in gentamycin-induced nephrotoxicity in rat models. Six groups of male Sprague-Dawley rats of eight weeks old were taken for the experiment: sham c...
In an attempt to make gentamycin delivery systems suitable for per-operative local antibiotic therapy, gentamycin base, GB, and gentamycin sulfate, GS, were blended with poly (dl-lactic acid) oligomers, PLA50p, dissolved in acetone. Waxy PLA50p oligomers were prepared by step-growth polymerizati...
We developed a sensitive, simple, inexpensive and rapid bead-based immunoassay platform, composed of liposomal nanovesicle amplification system, Gentamycin sulfate beads and 96-well filtration plates. In the beginning of the assay, Gentamycin sulfate beads, Gentamycin sulfate and Gentamycin spec...
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